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GB/T 35799-2018 PDF English

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GB/T 35799-2018: Determination of Sodium PCA in Cosmetics - High Performance Liquid Chromatography
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GB/T 35799-2018150 Add to Cart Auto, 9 seconds. Determination of Sodium PCA in Cosmetics - High Performance Liquid Chromatography Valid

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GB/T 35799-2018: Determination of Sodium PCA in Cosmetics - High Performance Liquid Chromatography


---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT35799-2018
GB NATIONAL STANDARD OF THE PEOPLE’S REPUBLIC OF CHINA ICS 71.100.70 Y 42 Determination of Sodium PCA in Cosmetics - High Performance Liquid Chromatography Issued on: FEBRUARY 6, 2018 Implemented on: SEPTEMBER 1, 2018 Issued by. General Administration of Quality Supervision, Inspection and Quarantine; Standardization Administration of the People’s Republic of China.

Table of Contents

Foreword ... 3 1 Scope ... 4 2 Normative References ... 4 3 Principle ... 4 4 Reagents and Materials ... 4 5 Instruments and Equipment ... 5 6 Steps of Determination ... 5 7 Result Calculation ... 7 8 Recovery Rate and Precision ... 7 9 Allowable Difference ... 7 Appendix A (Informative) Chinese Name, English Name, INCI Name, CAS No., Molecular Formula, Relative Molecular Mass and Structural Formula of Pyrrolidone Carboxylic Acid ... 8 Appendix B (Informative) HPLC Chromatogram and DAD Spectrogram of Pyrrolidone Carboxylic Acid Standards ... 9 Determination of Sodium PCA in Cosmetics - High Performance Liquid Chromatography

1 Scope

This Standard specifies the method of high performance liquid chromatography of determining sodium PCA in cosmetics (counted by pyrrolidone carboxylic acid). This Standard is applicable to the determination of sodium PCA (counted by pyrrolidone carboxylic acid) in creamy, milky and liquid cosmetics. In terms of sodium PCA (counted by pyrrolidone carboxylic acid), this Standard’s detection limit is 0.003%; its limit of quantitation is 0.01%.

2 Normative References

The following documents are indispensable to the application of this Standard. In terms of references with a specified date, only versions with a specified date are applicable to this Standard. The latest version (including all the modifications) of references without a specified date is applicable to this Standard. GB/T 6682 Water for Analytical Laboratory Use - Specification and Test Methods

3 Principle

Sample shall receive demulsification with saturated sodium chloride; mobile phase ultrasonic extraction and centrifugation; filtration with 0.45 μm filter. Inject solution into high performance liquid chromatograph; quantify with external standard method.

4 Reagents and Materials

Unless it is otherwise stipulated, all reagents shall be analytical purity. Water shall be Grade-1 water that is stipulated in GB/T 6682. 4.1 Phosphate. excellent level of purity. 4.2 Sodium chloride. 4.3 Methanol. chromatographic purity. 4.4 Potassium dihydrogen phosphate. 4.5 0.02 mol/L potassium dihydrogen phosphate (pH=3.0). weigh-take 2.72 g of potassium dihydrogen phosphate (4.4); add water to dissolve it; use phosphate (4.1) to adjust pH to 3.0; add water to dilute to the constant volume of 1,000 mL. Use 0.45 μm filter to filter it. 4.6 Saturated sodium chloride. weigh-take 35.2 g of sodium chloride (4.2); add 97.8 g of water; mix it, dissolve it for later usage. 4.7 Pyrrolidone carboxylic acid standards. purity shall be not less than 99.0%. Please refer to Table A.1 in Appendix A for its Chinese name, English name, INCI name, CAS No., molecular formula, relative molecular mass and structural formula. 4.8 Pyrrolidone carboxylic acid standard stock solution. accurately weigh-take 0.1 g of pyrrolidone carboxylic acid standards, accurate to 0.0001 g; place it in a 100 mL volumetric flask, then, use water to dissolve it to the constant volume. Thus, pyrrolidone carboxylic acid standard stock solution (concentration. 1,000 mg/L) can be obtained. Store it in a refrigerator at 4 °C; it shall remain valid within 1 week. 4.9 Standard working solutions. use 0.02 mol/L potassium dihydrogen phosphate (4.5) to separately prepare the above-mentioned standard stock solution (4.8) into a series of standard working solutions at the concentration of 0 mg/L, 2.0 mg/L, 10 mg/L, 50 mg/L, 100 mg/L and 200 mg/L; prepare it right before usage.

5 Instruments and Equipment

5.1 Liquid chromatograph, equipped with UV detector or diode array detector. 5.2 Analytical balance, division value. 0.0001 g. 5.3 Ultrasonic oscillator. 5.4 Centrifuge. rotating speed ≥ 10,000 r/min. 5.5 Filter membrane. aperture 0.45 μm. 5.6 Centrifuge tube. 10 mL. 5.7 Vortex oscillator.

6 Steps of Determination

6.1 Sample Processing Weigh-take 0.2 g (accurate to 0.001 g) of sample, place it into a 10 mL centrifuge tube. Add 1.0 mL of saturated sodium chloride (4.6), then, start vortex shaking for 1 min. Add 5.0 mL of 0.02 mol/L potassium dihydrogen phosphate (4.5). Start ultrasonic extraction ......
Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
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