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Preparation, preservation and management specification for nucleic acid of quarantine pest voucher specimens
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Basic data
| Standard ID | SN/T 4629-2016 (SN/T4629-2016) |
| Description (Translated English) | Preparation, preservation and management specification for nucleic acid of quarantine pest voucher specimens |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Classification of Chinese Standard | C62 |
| Word Count Estimation | 10,126 |
| Date of Issue | 2016-08-23 |
| Date of Implementation | 2017-03-01 |
| Regulation (derived from) | State-Quality-Inspection-Certification (2016)438 |
| Issuing agency(ies) | General Administration of Customs |
SN/T 4629-2016: Preparation, preservation and management specification for nucleic acid of quarantine pest voucher specimens
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Preparation, preservation and management specification for nucleic acid of quarantine pest voucher specimens
China's entry-exit inspection and quarantine industry standards
Preparation of nucleic acid for quarantine pest document specimens,
Conservation and Management Specifications
Published on.2016-08-23
2017-03-01 Implementation
China
The State Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Certification and Accreditation Regulatory Commission.
This standard is drafted by. China Academy of Inspection and Quarantine.
The main drafters of this standard. Song Yun, Chen Yan, Lu Jizhou, Zhao Wenjun, Wu Shaoqiang, Zhu Shuifang.
Preparation of nucleic acid for quarantine pest document specimens,
Conservation and Management Specifications
1 Scope
This standard specifies the requirements for the preservation and management of nucleic acids in quarantine pest document specimens.
This standard applies to the preservation and management of nucleic acid in specimens of quarantine pests.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article
Pieces. For undated references, the latest version (including all amendments) applies to this document.
SN/T 1193 Genetic Testing Laboratory Technical Requirements
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Voucherspecimens
Complete collection and identification information (collectors, dates, locations, habitats, appraisers, species names, etc.) for species review, citation, and traceability
And permanently preserved specimens.
3.2
Nucleic acid nucleic acid
Nucleic acid molecules fall into two categories. deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), which carry genetic information in cells.
4 Nucleic Acid Treatment
4.1 Common Equipment
For different sources of materials and samples using different treatment methods, commonly used equipment see Appendix A.
4.2 Nucleic Acid Sources
For insects, fungi, nematodes, bacteria, viruses, weeds, nucleic acids come from individuals or pure cultures of pests, viruses and phytoplasmas
Bacteria come from infected plant material.
4.3 Nucleic Acid Extraction
4.3.1 Nucleic Acid Release
4.3.1.1 Mechanical method
Including hypotonic cracking, ultrasonic cracking, microwave cracking, freeze-thaw cracking, liquid nitrogen grinding and particle crushing physical cracking methods. These methods use
Mechanical forces break the cells.
...