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Methods for examination of import microbial blends in the environmental protection--Part 14: Aspergillus ustus
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Basic data
| Standard ID | SN/T 4624.14-2016 (SN/T4624.14-2016) |
| Description (Translated English) | Methods for examination of import microbial blends in the environmental protection--Part 14: Aspergillus ustus |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Classification of Chinese Standard | C62 |
| Word Count Estimation | 10,145 |
| Date of Issue | 2016-08-23 |
| Date of Implementation | 2017-03-01 |
| Regulation (derived from) | State-Quality-Inspection-Certification (2016)438 |
| Issuing agency(ies) | General Administration of Customs |
SN/T 4624.14-2016: Methods for examination of import microbial blends in the environmental protection--Part 14: Aspergillus ustus
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Test method for microbial inoculants for entry into the environment - Part 14. Aspergillus)
People's Republic of China Exit Inspection and Quarantine standards
Inbound environmental testing methods for microbial agents
Part 14. Fusarium graminearum
environmentalprotection-Part 14.Aspergillusustus
2016-08-23 released
2017-03-01 Implementation
People's Republic of China
General Administration of Quality Supervision, Inspection and Quarantine released
Exit Inspection and Quarantine of People's Republic of China
Industry Standard
Inbound environmental testing methods for microbial agents
Part 14. Fusarium graminearum
China Standard Press
First edition of November.2017
Foreword
SN/T 4624 "inbound environmental testing methods using microbial agents" is divided into 17 parts.
--- Part 1. Bacillus licheniformis;
--- Part 2. Bacillus pumilus;
--- Part 3. Bacillus megaterium;
--- Part 4. Acidithiobacillus ferrooxidans;
--- Part 5. β-hemolytic streptococcus;
--- Part 6. Staphylococcus aureus;
--- Part 7. Salmonella;
--- Part 8. Shigella licheniformis;
--- Part 9. Diarrhea caused by Escherichia coli;
--- Part 10. Paecilomyces lilacinus;
--- Part 11. Elegant small ginkgo mildew;
--- Part 12. Trichoderma harzianum;
--- Part 13. Phanerochaete chrysosporium;
--- Part 14. Curvularia;
--- Part 15. Bacillus amyloliquefaciens;
--- Part 16. Alkaloid-like-producing Pseudomonas;
--- Part 17. Pseudomonas putida.
This section is part 14 of SN/T 4624.
This section drafted in accordance with GB/T 1.1-2009 given rules.
This part of the national certification and accreditation supervision and management committee proposed and centralized.
This part of the drafting unit. Liaoning Entry-Exit Inspection and Quarantine of People's Republic of China, Exit Inspection and Quarantine of Hebei.
The main drafters of this section. Zhang Ying, Zhou Jiannan, Li Yun, Liu Xin, Li Yao, Fu Yang, Wang Fang.
Inbound environmental testing methods for microbial agents
Part 14. Fusarium graminearum
1 Scope
SN/T 4624 provisions of this part of the entry of environmental Microbiological Microbiological Compliance test Aspergillus morphology identification, real-time fluorescence
PCR detection method.
This section applies to the entry of environmentally friendly microbial inoculum test for the detection and identification of Aspergillus.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 analytical laboratory water specifications and test methods
3 main reagents and medium
3.1 Experimental water should be consistent with a water GB/T 6682 specifications.
3.2 Charles's agar See Appendix A, A.1.
3.3 Char's broth. See A.2.
3.4 CTAB buffer. See A.3.
3.5 Protease K.
3.6 Tris saturated phenol.
3.7 trichloromethane.
3.8 Isoamyl alcohol.
3.9 isopropanol.
3.10 TE buffer (pH 8.0). See A.4.
3.11 70% ethanol.
3.12 ethidium bromide.
3.13 DNA molecular weight marker .1000bp DNAladder.
3.14 agarose.
3.15 50 × TAE buffer (pH 8.5). See A.5.
3.16 RealMasterMix (2.5 ×, ROX containing reference dye).
3.17 20 × ProbeEnhancerSolution.
3.18 liquid nitrogen.
4 major equipment and materials
4.1 electronic balance (sense of volume 0.01g).
...
