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Real-time RT-PCR method for Mayaro virus detection at frontier ports
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Basic data
| Standard ID | SN/T 4614-2016 (SN/T4614-2016) |
| Description (Translated English) | Real-time RT-PCR method for Mayaro virus detection at frontier ports |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Classification of Chinese Standard | C62 |
| Word Count Estimation | 6,661 |
| Date of Issue | 2016-08-23 |
| Date of Implementation | 2017-03-01 |
| Regulation (derived from) | State-Quality-Inspection-Accredidation (2016) No.438 |
| Issuing agency(ies) | General Administration of Customs |
SN/T 4614-2016: Real-time RT-PCR method for Mayaro virus detection at frontier ports
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Detection of Maya virus by RT - PCR at border crossings)
People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard
Frontier port Maya virus fluorescence
RT-PCR detection method
Published on.2016-08-23
2017-03-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Entry and exit inspection and quarantine
Industry Standard
Frontier port Maya virus fluorescence
RT-PCR detection method
China Standard Press Publishing
First edition in December.2017
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. China Academy of Inspection and Quarantine.
The main drafters of this standard. Sun Xiaohong, Ma Xuezheng, Liu Jian, Xu Baoliang, Bai Hongyan, Zhang Liping.
Frontier port Maya virus fluorescence
RT-PCR detection method
1 Scope
This standard specifies the biosafety requirements for Maya virus detection, collection, transportation and preservation of specimens, specimen processing and fluorescence RT-
PCR detection.
This standard applies to the fluorescent RT-PCR detection of Maya virus.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB 19489 General requirements for laboratory biosafety
List of pathogenic microorganisms transmitted from humans (Wuwei Education and Development [2006] No. 15 of the Ministry of Health of the People's Republic of China)
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Maya virus Mayarovirus; MAYV
Maya virus is a mosquito-borne arbovirus, belonging to the genus of the virus of the genus togavirus. The blood-sucking mosquito is its main vector and is mainly popular.
In Australia. The clinical manifestations of human infection are similar to dengue fever, fever, chills, headache, eye pain, rash and arthritis, in the absence of experimentation
Room diagnosis is easily confused with dengue fever.
4 Abbreviations
The following abbreviations apply to this document.
Ct value The number of cycles experienced when the fluorescent signal in each reaction tube reaches a set threshold;
FAM FAM fluorescent dye, a fluorescent reporter group;
RNA ribonucleic acid;
RT-PCR reverse transcription-polymerase chain reaction.
5 Biosafety requirements
The "List of Pathogenic Microorganisms Infected by Humans" issued by the Health and Family Planning Commission of the People's Republic of China stipulates that the Maya virus is not cultivated.
The handling of infectious and inactivated materials should be carried out at different levels of biosafety laboratories. details as follows.
--- Operation of Maya virus-associated infectious materials should be performed in Bio-Annual (BSL-2 or ABSL-2) laboratories;
--- Maya virus-related inactivation materials are carried out in the Biosafety Level 1 (BSL-1) laboratory;
--- Maya virus infectious material transport packaging is classified as Class B, UN number is UN3373.
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