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Real-time RT-PCR method for Kunjin virus detection at frontier ports
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Basic data
| Standard ID | SN/T 4613-2016 (SN/T4613-2016) |
| Description (Translated English) | Real-time RT-PCR method for Kunjin virus detection at frontier ports |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Classification of Chinese Standard | C62 |
| Word Count Estimation | 6,657 |
| Date of Issue | 2016-08-23 |
| Date of Implementation | 2017-03-01 |
| Regulation (derived from) | State-Quality-Inspection-Certification (2016)438 |
| Issuing agency(ies) | General Administration of Customs |
SN/T 4613-2016: Real-time RT-PCR method for Kunjin virus detection at frontier ports
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Real-time RT-PCR method for Kunjin virus detection at frontier ports
China's entry-exit inspection and quarantine industry standards
Quantitative RT-PCR detection method for Kunjin virus at border ports
Published on.2016-08-23
2017-03-01 Implementation
China
The State Administration of Quality Supervision, Inspection and Quarantine issued
Inspection and Quarantine of the People's Republic of China
Industry Standard
Quantitative RT-PCR detection method for Kunjin virus at border ports
China Standard Press Publishing
First edition of January.2018
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Certification and Accreditation Regulatory Commission.
This standard was drafted by. China Academy of Inspection and Quarantine, Hebei International Travel Health Care Center.
The main drafters of this standard. Sun Xiaohong, Liu Jian, Yan Yihuan, Xu Baoliang, Ma Xuezheng, Zhang Liping, Bai Hongyan.
Quantitative RT-PCR detection method for Kunjin virus at border ports
1 Scope
This standard specifies the biosafety requirements for the detection of Kunming virus, the collection, transportation, and storage of specimens, the processing of specimens, and fluorescence RT-
PCR detection.
This standard applies to the fluorescence RT-PCR detection of Kunjin virus.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article
Pieces. For undated references, the latest version (including all amendments) applies to this document.
GB 19489 General Requirements for Laboratory Biosafety
List of Pathogens of Human Infection (Ministry of Health, Health Science, Education and Education [2006] No. 15)
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Kunjin virus Kunjinvirus; KUNV
It can also be translated as “Kutchin Virus”, a member of the Flaviviridae Flavivirus genus, and in Culexan in Australia in 1960.
First isolated in nulirostris). In addition to Culex pipiens pallens, Culex seudovishnui, Culex quinquefasciatus (Culex)
Quinquefasciatus), Culex australis and Culex suamosus can also spread the disease
poison. The main hosts of the virus are birds, poultry, pigs, cattle, and horses. Waterfowls can spread the virus to distant places, while pigs, cows and horses can enlarge
The role of the host. Kunjin virus disease is a zoonotic disease, mainly in Australia. After human infection, it mainly manifests as fever, headache,
Muscle pain, severe cases can develop encephalitis.
4 Abbreviations
The following abbreviations apply to this document.
Ct value The number of cycles experienced when the fluorescence signal within each reaction tube reaches the set threshold;
FAM FAM fluorescent dye, a fluorescent reporter group;
RNA RNA
RT-PCR reverse transcription-polymerase chain reaction.
5 Biosafety requirements
The List of Pathogens Infected by Humans stipulates that the operation of uninfected infectious substances and inactivated materials of Kunjin virus should be different.
Level biosafety laboratories, as follows.
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