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SN/T 4611-2016: Nested PCR detecting method for spotted fever group rickettsiae at frontier port
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SN/T 4611-2016	English	139	Add to Cart	3 days [Need to translate]	Nested PCR detecting method for spotted fever group rickettsiae at frontier port

SN/T 4611-2016: Nested PCR detecting method for spotted fever group rickettsiae at frontier port

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Nested PCR detecting method for spotted fever group rickettsiae at frontier port China's entry-exit inspection and quarantine industry standards Spotted thermal group Rickettsia Nested PCR detection method Published on.2016-08-23 2017-03-01 Implementation China The State Administration of Quality Supervision, Inspection and Quarantine issued Inspection and Quarantine of the People's Republic of China Industry Standard Spotted thermal group Rickettsia Nested PCR detection method China Standard Press Publishing The first edition of November.2017

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. The issuing agency of this document does not assume responsibility for identifying these patents. This standard is proposed and managed by the National Certification and Accreditation Regulatory Commission. This standard was drafted by. Heilongjiang Entry-Exit Inspection and Quarantine Bureau, People's Republic of China Bureau, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences. The main drafters of this standard. Yu Wendong, Wang Yanmei, Ding Shuli, Cheng Cheng, Yan Yihuan, Xu Ning, Fu Weiming, Yu Cong, Sun Yi, Bao Li. Spotted thermal group Rickettsia Nested PCR detection method

1 Scope

This standard specifies the inspection objects, methods, biosafety requirements, and results determination of spotted heat populations of rickettsia at the border ports. This standard is applicable to nests of people at border crossings, medical vector organisms (蜱, 螨, etc.) and spotted fever group rickettsia in host animals. The PCR method was used to detect four Rickettsia species except R.helvetica, R.australis, R.bellii and R.canadensis.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article Pieces. For undated references, the latest version (including all amendments) applies to this document. GB/T 6682 Analysis Laboratory Water Specifications and Test Methods GB 19489 General Requirements for Laboratory Biosafety SN/T 1193 Genetic Testing Laboratory Technical Requirements Application of polymerase chain reaction (PCR) in clinical diagnosis of WS/T 230

3 Terms and Definitions

The following terms and definitions apply to this document. 3.1 Spotted fever group Rickettsia spottedfevergrouprickettsiae;SFGR A group of pathogens causing spotted fever can cause spotty heat including Rocky Mountain, button fever, North Asian fever, Queensland spot fever, and Rickettsia vaccination Many diseases, including Japan's erythema fever, are natural parasites that are parasitized in lice and cockroaches and transmitted to humans or animals through the bite of cockroaches and cockroaches. Cellular parasites. Except for individual rickettsial worms, the rest are pupa transmission, with obvious natural plague characteristics. The main media The mediation includes. prairie leather clams, edge leather clams, forest leather clams, Chinese leather clams, gregarious blood clams, Japanese blood clams, long-horned blood clams, and Asian glazed clams.

4 Test object

4.1 Blood samples from suspected suspects infected with spotted fever rickettsia. 4.2 Free enthalpy of rickettsia from suspected spotted fever group collected from entry and exit vehicles, containers and port areas, etc. Parasites collected on the surface of host animals in the site. 4.3 Infested raccoa host animals suspected of being spotted by inbound and outbound vehicles, containers and port areas. 5 Biosafety and PCR anti-pollution requirements 5.1 The personal protection in the inspection work is performed according to GB 19489. 5.2 Laboratory for specimen testing should meet the requirements of GB 19489 Biosafety Level 2 Laboratory. 5.3 The used experimental articles shall be treated as harmless according to the provisions of GB 19489. ...