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SN/T 4276-2015: Detection of Yersinia pestis in rodents by real-timePCR method at frontier port
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SN/T 4276-2015: Detection of Yersinia pestis in rodents by real-timePCR method at frontier port

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Detection of Yersinia pestis in rodents by real-timePCR method at frontier port People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Yersinia pestis fluorescence at border port PCR detection method Released on.2015-05-26 2016-01-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued Entry and exit inspection and quarantine Industry Standard Yersinia pestis fluorescence at border port PCR detection method China Standard Press Publishing First edition in January.2016

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Beijing Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Beijing Haidian District Center for Disease Control and Prevention, China Fujian Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Tian Jie, Lu Lin, Guo Huilin, He Bin, Liu Yanhua, Ren Yi, Zhao Jing, Huang Enzhen. Yersinia pestis fluorescence at border port PCR detection method

1 Scope

This standard specifies the detection object of the fluorescent PCR detection method of Yersinia pestis in the border port, the collection, processing and detection of the specimen. program. This standard applies to the fluorescent PCR detection of Yersinia pestis in border ports.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB 19489 General requirements for laboratory biosafety GB/T 28940 vector test for pathogens of vector infections GB/T 28942 Sampling protocol for pathogens of vector infections蚤 SN/T 1240 rodent monitoring procedures at border ports SN/T 1292 Monitoring procedures for scorpion at border ports WS279 Plague Diagnostic Criteria List of pathogenic microorganisms transmitted from humans (Ministry of Health)

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Yersiniapestis Commonly known as plague bacillus, is a Gram-negative crude bacillus, the ends of the bacteria are blunt and densely stained, and are also easily stained with aniline dye, the size is (0.5 μm to 1.0 μm) × (1.0 μm to 2.0 μm). Generally dispersed, occasionally double or short chain, no flagella, no spore formation. Yersinia pestis is the pathogen of plague. The plague is a natural epidemic infectious disease that is common to humans and animals. The human plague is mostly the voles of the plague. Infected by bites, it is a legal type A infectious disease in China. The plague is highly contagious and has a high mortality rate, which is easy to cause a pandemic. Yersinia pestis To affect the skin and lymph nodes, followed by sepsis, pneumonia, meningitis.

4 Abbreviations

The following abbreviations apply to this document. PCR. polymerase chain reaction (polymerase chain reaction) Ct value. cycle threshold, the number of cycles experienced by each reaction tube's fluorescence signal reaching a set threshold (cyclethreshold) DNA. deoxyribonucleic acid PBS buffer ...