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SN/T 4274-2015: Detection of Salmonella, Shigella and Escherichia coli 0157: H7 by triplex real-time PCR at frontier ports
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SN/T 4274-2015: Detection of Salmonella, Shigella and Escherichia coli 0157: H7 by triplex real-time PCR at frontier ports

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Detection of Salmonella, Shigella and Escherichia coli 0157. H7 by triplex real-time PCR at frontier ports People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Borderline Salmonella, Shigella, Escherichia coli Triple fluorescent PCR detection method for O157.H7 DetectionofSalmonella, ShigellaandEscherichiacoli Released on.2015-05-26 2016-01-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Sichuan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shandong Entry-Exit Inspection and Quarantine Bureau, Shanghai Zhijiang Biotechnology Co., Ltd. The main drafters of this standard. Fan Xuejun, Tian Lubo, Wang Ying, Chen Xiaoyu, Shi Ying, Yang Yu, Ni Weiqin, Zhu Xuping, Wang Ning. Borderline Salmonella, Shigella, Escherichia coli Triple fluorescent PCR detection method for O157.H7

1 Scope

This standard specifies the subjects of Salmonella, Shigella, Escherichia coli O157.H7 triple fluorescent PCR detection at the border ports, specimens Collection, transportation and storage, testing procedures and results reporting. This standard is applicable to the screening and testing of Salmonella, Shigella and E. coli O157.H7 at border ports.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 4789.17 Food hygiene microbiological examination meat and meat products inspection GB 4789.18 National Food Safety Standards Food Microbiological Testing Milk and Dairy Products Inspection GB/T 4789.19 Food hygiene microbiological examination egg and egg product inspection GB/T 4789.20 Food hygiene microbiological examination aquatic food inspection GB/T 4789.21 Microbiological examination of food hygiene, frozen drinks, beverage inspection GB/T 4789.22 Food hygiene microbiological examination condiment inspection GB/T 4789.23 Food hygiene microbiological examination cold food, bean products inspection GB/T 4789.24 Microbiological examination of food hygiene, inspection of confectionery, cakes and preserves GB 19489 General requirements for laboratory biosafety

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Salmonella Salmonella Is Enterobacteriaceae, Gram-negative bacilli. Cell size (0.7μm~1.5μm)×(2.0μm~5.0μm), no spores, generally no The capsule, except for Salmonella pullorum and Salmonella typhimurium, mostly has flagella. Including those that cause food poisoning, leading to gastroenteritis, Typhoid and paratyphoid bacteria. It can even lead to severe dehydration and acute kidney failure. 3.2 Shigella Shigella Is Enterobacteriaceae, Gram-negative bacilli. Size is (0.5μm~0.7μm) × (2μm~3μm), no spore, no capsule, no whip hair. Most have pili. It is the most common pathogen of human bacterial dysentery. The disease caused by Shigella is more serious, with systemic poisoning Symptoms and lower colonic suppurative inflammation are the main features, and severe cases can occur with septic shock and/or toxic encephalopathy. 3.3 E. coli O157.H7 EscherichiacoliO157.H7 It is an Enterobacteriaceae belonging to the genus Escherichia, and is a Gram-negative bacterium. Size (0.4μm~0.7μm) × (1μm~3μm), No spores, common pili and sexual pili. Can cause diarrhea, hemorrhagic colitis, 5% to 10% of cases complicated by hemolytic uremic syndrome ...