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Identification of poultry ingredient in food and feed. Part 6: Detection of alectoris ingredient-Real-tiem PCR method
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Basic data
| Standard ID | SN/T 3731.6-2013 (SN/T3731.6-2013) |
| Description (Translated English) | Identification of poultry ingredient in food and feed. Part 6: Detection of alectoris ingredient-Real-tiem PCR method |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Classification of Chinese Standard | X18 |
| Classification of International Standard | 67.120 |
| Word Count Estimation | 8,897 |
| Quoted Standard | GB/T 6682; GB/T 27403 |
| Regulation (derived from) | National quality recognition (2013) 569 |
| Issuing agency(ies) | General Administration of Customs |
| Summary | This standard specifies the food and feed Partridge (Chukar) components of real-time PCR detection methods. This standard applies to food and feed partridge qualitative detection (partridge) components. |
SN/T 3731.6-2013: Identification of poultry ingredient in food and feed. Part 6: Detection of alectoris ingredient-Real-tiem PCR method
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Identification of poultry ingredient in food and feed Part 6. Detection of alectoris ingredient-Real-tiem PCR method
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Identification of common food and feed birds species
Part 6. Components of partridge
Real-time PCR method
Part 6. Detectionofalectorisingredient-Real-tiemPCRmethod
Issued on. 2013-11-06
2014-06-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 3731 "Identification of Food and Feed common bird species" is divided into the following six sections.
--- Part 1. quail component detecting PCR method;
--- Part 2. Geese component detecting PCR method;
--- Part 3. Pigeon component detecting real-time PCR method;
--- Part 4. Components of turkey real-time PCR method;
--- Part 5. duck component detecting PCR method;
--- Part 6. partridge component detecting real-time PCR method.
This section SN/T Section 63731 of.
This section drafted in accordance with GB/T 1.1-2009 given rules.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This section drafted by. Chinese Academy of Inspection and Quarantine, Shenzhen People's Republic of China Exit Inspection and Quarantine, Shenzhen test
Quarantine Science Research Institute.
The main drafters of this section. Caochen Fu Zong Hui, Yangjun Xing, Lu Kang body, flower Qun Yi, Qin Zhifeng, Lv Jianjiang Ye Yi gifted, Zhang Caihong, TAO,
Hanjian Xun.
Identification of common food and feed birds species
Part 6. Components of partridge
Real-time PCR method
1 Scope
SN/T 3731 provisions of this part of the food and feed Partridge (partridge) component detected by real-time PCR method.
This section applies to food and feed in the qualitative detection Partridge (partridge) component.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 27403 laboratory quality control of food molecular biology standardized testing
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Real-time fluorescence PCR realtimePCR
Join fluorophore in the PCR reaction system, the use of real-time monitoring of the accumulation of the fluorescent signal of the PCR amplification process.
3.2
Ct values cyclethreshold
The number of cycles when fluorescence signal of each reaction tube reaches a set threshold experienced.
4 Abbreviations
The following abbreviations apply to this document.
DNA. deoxyribonucleic acid (deoxyribonuleicacid)
dNTP. deoxyribonucleoside triphosphate (deoxy-ribonucleosidetriphosphate)
PCR. polymerase chain reaction (polymerasechainreaction)
ROX. 6- carboxy -X- rhodamine (6-carboxyl-X-Rhodamine)
CTAB. cetyl trimethyl ammonium bromide (cetyltrithylammoniumbromide)
Tris. Tris (hydroxymethyl) aminomethane (tris (hydroxymethyl) aminomethane)
EDTA. ethylenediaminetetraacetic acid (ethylenediaminetetraaceticacid)
5 Method summary
The sample was milled after mixing, extracting DNA, DNA as template, partridge mitochondrial CoI gene-specific primers and probes to detect
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