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Identification of poultry ingredient in food and feed. Part 5: Detection of duck ingredient. PCR method
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Basic data
| Standard ID | SN/T 3731.5-2013 (SN/T3731.5-2013) |
| Description (Translated English) | Identification of poultry ingredient in food and feed. Part 5: Detection of duck ingredient. PCR method |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Classification of Chinese Standard | X18 |
| Classification of International Standard | 67.120 |
| Word Count Estimation | 8,852 |
| Quoted Standard | GB/T 6682; GB/T 27403 |
| Regulation (derived from) | National quality recognition (2013) 569 |
| Issuing agency(ies) | General Administration of Customs |
| Summary | This standard specifies the method for food and feed ducks PCR measurements of polar components. This standard applies to the qualitative detection of duck food and feed ingredients. |
SN/T 3731.5-2013: Identification of poultry ingredient in food and feed. Part 5: Detection of duck ingredient. PCR method
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Identification of poultry ingredient in food and feed Part 5. Detection of duck ingredient PCR method.
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Identification of common food and feed birds species
Part 5. duck component detection by PCR
Part 5. Detectionofduckingredient-PCRmethod
Issued on. 2013-11-06
2014-06-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 3731 "Identification of Food and Feed common bird species" is divided into the following six sections.
--- Part 1. quail component detecting PCR method;
--- Part 2. Geese component detecting PCR method;
--- Part 3. Pigeon component detecting real-time PCR method;
--- Part 4. Components of turkey real-time PCR method;
--- Part 5. duck component detecting PCR method;
--- Part 6. partridge component detecting real-time PCR method.
This section SN/T Section 53731 of.
This section drafted in accordance with GB/T 1.1-2009 given rules.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This section drafted by. Chinese Academy of Inspection and Quarantine, Shenzhen People's Republic of China Exit Inspection and Quarantine, Shenzhen test
Quarantine Science Research Institute.
The main drafters of this section. Zong Hui, Wu Yajun, TAO, meaning flower group, Caochen Fu, Huang Wensheng, Juan, Liu Jianli, Lv Jianqiang, Lu Kang body.
Identification of common food and feed birds species
Part 5. duck component detection by PCR
1 Scope
This section SN/T 3731 provisions of the PCR method of food and feed ingredients duck very measured.
This section applies to the qualitative detection of food and feed ingredients duck.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 27403 laboratory quality control of food molecular biology standardized testing
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Polymerase chain reaction polymerasechainreaction; PCR
Method for amplifying DNA in vitro. PCR was performed using a heat-resistant polymerase, and two single-stranded primer containing about 20 bases
Thereof. After separating the high temperature denaturation of template DNA into two strands, so that low-temperature annealing of primers and a combination of single-stranded template and then extended temperature
Extension, the reaction was followed by the free nucleotide primers from the 5 'to 3' synthesis of a new complementary strand. The newly synthesized DNA and can continue
Continued above-described cycle, so that the number of DNA continue to multiply.
3.2
TaqDNA enzyme ThermusaquaticusDNApolymerase
Thermusaquaticus bacteria extracted from the thermostable DNA polymerase.
4 Abbreviations
The following abbreviations apply to this document.
dNTP. deoxyribonucleoside triphosphate (deoxy-ribonucleosidetriphosphate)
CTAB. cetyl trimethyl ammonium bromide (cetyltrithylammoniumbromide)
Tris. Tris (hydroxymethyl) aminomethane [tris (hydroxymethyl) aminomethane]
EDTA. ethylenediaminetetraacetic acid (ethylenediaminetetraaceticacid)
DNAMarker. nucleic acid molecular weight standards
5 Method summary
The samples were mixed grinding, extracting DNA, DNA as a template, using duck mitochondrial cytochrome C oxidase Ⅲ gene-specific detection
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