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| SN/T 3731.4-2017 | English | 169 |
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Identification of poultry ingredient in food and feed - Part 4: Detection of turkey ingredient - Real-time PCR method
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| SN/T 3731.4-2013 | English | 319 |
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Identification of poultry ingredient in food and feed. Part 4: Detection of turkey ingredient. Real-time PCR method
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Basic data
| Standard ID | SN/T 3731.4-2017 (SN/T3731.4-2017) |
| Description (Translated English) | Identification of poultry ingredient in food and feed - Part 4: Detection of turkey ingredient - Real-time PCR method |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Classification of Chinese Standard | X18 |
| Classification of International Standard | 67.120 |
| Word Count Estimation | 7,757 |
| Date of Issue | 2017-07-21 |
| Date of Implementation | 2018-03-01 |
| Older Standard (superseded by this standard) | SN/T 3731.4-2013 |
| Quoted Standard | GB 6682; GB/T 27403 |
| Regulation (derived from) | State-Quality-Inspection-Accreditation (2017) 337 |
| Issuing agency(ies) | General Administration of Customs |
| Summary | This standard specifies the real-time fluorescent PLR detection method for turkey components in food and feed. This standard applies to the qualitative detection of turkey ingredients in food and feed. |
SN/T 3731.4-2017: Identification of poultry ingredient in food and feed - Part 4: Detection of turkey ingredient - Real-time PCR method
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Identification of poultry ingredient in food and feed - Part 4. Detection of turkey ingredient - Real-time PCR method
People's Republic of China entry and exit inspection and quarantine industry standards
Replace SN/T 3731.4-2013
Method for identification of common poultry species in food and feed
Part 4. Detection of turkey ingredients
Real-time fluorescent PCR
Part 4. Detectionofturkeyingredient-Real-timePCRmethod
Released on.2017-07-21
2018-03-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
SN/T 3731 "Methods for Identification of Common Poultry Varieties in Food and Feed" is divided into the following six parts.
--- Part 1. 鹌鹑 component detection PCR method;
--- Part 2. Goose component detection PCR method;
--- Part 3. Real-time fluorescent PCR method for pigeon component detection;
--- Part 4. Real-time fluorescent PCR method for turkey component detection;
--- Part 5. Duck component detection PCR method;
--- Part 6. Real-time fluorescent PCR method for the detection of sputum components.
This part is the fourth part of SN/T 3731.
This part is drafted in accordance with the rules given in GB/T 1.1-2009.
Compared with SN/T 3731.4-2013, the main technical changes in this section are as follows.
--- Detection primers and probe sequences.
This part is proposed and managed by the National Certification and Accreditation Administration.
This section drafted by. China Academy of Inspection and Quarantine, Shanghai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this section. Zhang Shuya, Lu Rong, Qi Hongchao, Han Jianxun, Liu Yueming, Yan Qinghua, Chen Ying, Wu Yajun.
The previous versions of the standards replaced by this section are.
---SN/T 3731.4-2013.
Method for identification of common poultry species in food and feed
Part 4. Detection of turkey ingredients
Real-time fluorescent PCR
1 Scope
This part of SN/T 3731 specifies real-time fluorescent PCR detection methods for turkey components in food and feed.
This section applies to the qualitative detection of turkey ingredients in food and feed.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB 6682 Analytical laboratory water specifications and test methods
GB/T 27403 Laboratory Quality Control Specification for Food Molecular Biology Testing
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Turkey turkey
It belongs to the genus of the genus Pheadianidae (Meleagris) and its scientific name is Meleagrisgallopavo.
3.2
Real-time fluorescent PCR realtimePCR
A fluorescent group is added to the PCR reaction system, and the entire PCR amplification process is monitored in real time using the accumulation of fluorescent signals.
3.3
Ct value cyclethreshold
The number of cycles experienced by the fluorescent signal in each reaction tube reaching the set domain value.
4 Abbreviations
The following abbreviations apply to this document.
PCR. Polymerase Chain Reaction (PolymeraseChainReaction)
DNA. Deoxyribonuleic Acid
dNTP. Deoxyribonucleoside Triphosphate
CTAB. Cetyltrithylammonium Bromide
Tris. Tris (Hydroxymethyl) Aminomethane
EDTA. EthyleneDiaminetetraacetic Acid
Taq. Thermusaquaticus
...
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