Path:
Home >
SN/T >
Page73 > SN/T 3296-2012
| Std ID | Version | USD | Buy | Deliver [PDF] in | Title (Description) |
| SN/T 3296-2012 | English | 519 |
Add to Cart
|
3 days [Need to translate]
|
Criterion on detection of plant pathogenic bacteria by molecular biology
|
Click to Preview a similar PDF
Basic data
| Standard ID | SN/T 3296-2012 (SN/T3296-2012) |
| Description (Translated English) | Criterion on detection of plant pathogenic bacteria by molecular biology |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Classification of Chinese Standard | B16 |
| Classification of International Standard | 07.080 |
| Word Count Estimation | 13,159 |
| Quoted Standard | GB/T 27403; SN/T 1193; SN/T 2601 |
| Regulation (derived from) | National Quality Inspection (2012) 643; industry standard filing Notice 2013 No. 1 (No. 157 overall) |
| Issuing agency(ies) | General Administration of Customs |
| Summary | This standard specifies the molecular biology of plant pathogenic bacteria norms. This standard applies to bacterial diseases of plants and their products, purification of molecules quarantine and identification of plant pathogenic bacteria. |
SN/T 3296-2012: Criterion on detection of plant pathogenic bacteria by molecular biology
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Criterion on detection of plant pathogenic bacteria by molecular biology
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Plant pathogenic bacteria molecular biology Specification
Issued on. 2012-10-23
2013-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Liaoning Entry Inspection and Quarantine Bureau, People's Republic of China Shandong Entry-Exit Inspection and
Quarantine Bureau.
The main drafters of this standard. Shao Xiuling, Wangyou Fu, Nepal Xiumei, Li Yan, Ganqin Hua, Li Xin, Liu Hui Qiu, Wang Xiaoming.
Plant pathogenic bacteria molecular biology Specification
1 Scope
This standard specifies the molecular biology of plant pathogenic bacteria testing standards.
This standard applies to products of plant and bacterial diseases, separation and purification of molecules quarantine and identification of plant pathogenic bacteria.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB/T 27403 laboratory quality control of food molecular biology standardized testing
SN/T 1193 genetic testing laboratory technical requirements
SN/T 2601 plant pathogenic bacteria routine standardized testing
Principle 3
Depending on the plant pathogenic bacterium genome specific sequences, using molecular biology methods for pathogen identification.
According to the degree of pathogenic bacteria infection incidence and detection of target pathogenic bacteria and biological characteristics of plants with different degree of difficulty to distinguish between closely related species not
With, choose a different molecular methods for identification.
4 Reagents and Consumables
4.1 Reagents
Plant genome extraction kit, bacterial DNA extraction kit, ddH2O, Taq enzyme, of dNTP, commonly used fluorescent antibody, urea, demineralized
Son formamide, acrylamide, methylene bis-acrylamide, agarose, physiological saline; medium common bacterial isolates cultured see sidebar recipe
Record A. Unless otherwise specified, all reagents were of analytical grade or biochemical reagents.
4.2 Supplies
Petri dishes, test tubes, flasks, mortar tubes (1.5mL, 1.0mL), PCR tubes (0.2μL), graduated cylinders, beakers scissors, disinfection
Cylinder and so on.
5 Equipment
5.1 conventional equipment
Plant incubator, incubator, temperature oscillation incubator, autoclave, clean benches, biological microscope (with camera system), high
Speed refrigerated centrifuge (12000r/min), a small centrifuge, water meter, water bath pot, deep freezer, electric mixer, etc.
5.2 PCR method for major equipment
PCR, quantitative PCR instrument, electrophoresis, denaturing gradient gel electrophoresis, electrophoresis tank, capillary electrophoresis, gel image analysis
...
