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Molecular detection and identification of cotton leaf crumple virus
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Basic data
| Standard ID | SN/T 3287-2012 (SN/T3287-2012) |
| Description (Translated English) | Molecular detection and identification of cotton leaf crumple virus |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Classification of Chinese Standard | B16 |
| Classification of International Standard | 07.080 |
| Word Count Estimation | 13,131 |
| Regulation (derived from) | National Quality Inspection (2012) 643; industry standard filing Notice 2013 No. 1 (No. 157 overall) |
| Issuing agency(ies) | General Administration of Customs |
| Summary | This standard specifies the wrinkled cotton leaf virus (see Appendix A) nucleic acid extraction, PCR and real-time PCR detection method. This standard applies to the entry and exit phytosanitary wrinkled cotton leaf virus in quarantine and identification. |
SN/T 3287-2012: Molecular detection and identification of cotton leaf crumple virus
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Molecular detection and identification of cotton leaf crumple virus
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Wrinkled cotton leaf virus quarantine and identification of molecular
Issued on. 2012-10-23
2013-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. China Inspection and Quarantine Science Research Institute, People's Republic of China Zhejiang Exit Inspection and Quarantine, the People's Republic of China
State and Guangdong CIQ.
The main drafters. Zhang Yongjiang, Zhang Mingzhe, Liming Fu, Feng Lixia, MATURAL.
Wrinkled cotton leaf virus quarantine and identification of molecular
1 Scope
This standard specifies the method for extracting wrinkled cotton leaf virus (see Appendix A) nucleic acids, PCR and real-time PCR detection.
This standard applies to the entry and exit inspection and identification of plant quarantine in wrinkled cotton leaf virus.
2 principle of the method
Wrinkled cotton leaf virus Scientific name. Cottonleafcrumplevirus, Abbreviation. CLCrV. Molecular characterization of the virus is present standard
The main basis for making.
3 equipment, appliances, and reagents
3.1 Equipment
PCR, electrophoresis system, high-speed refrigerated centrifuge, gel imaging system, oscillators, 4 ℃ refrigerator, electronic analytical balance (0.001g), super
Net table, water bath, -80 ℃ ultra-low temperature refrigerators, autoclaves, ice maker, microwave.
3.2 Appliances
Adjustable pipette (2.5μL, 10μL, 20μL, 100μL, 1000μL) and sterile pipette tips, centrifuge tubes, PCR tubes and mortar and the like.
3.3 Reagents
Unless otherwise stated, all test reagents were analytical grade or biochemical reagents.
Real-time PCR reagents, chloroform, isoamyl alcohol, ethanol, TaqDNA polymerase, DNA molecular markers, ethidium bromide, agar
Sugar.
Reagent preparation see Appendix B.
4 identification of quarantine
4.1 conventional PCR detection
Extracted sample DNA, positive controls, negative control and blank control, conventional PCR amplification. Specific steps Mitsuke
Record C.
4.2 real-time PCR detection
Extracted sample DNA, positive controls, negative control and blank control, real-time fluorescence PCR amplification. See specific steps
Appendix D.
Analyzing the results of 5
Samples were PCR and real-time PCR assay was negative, it is determined the sample does not carry CLCrV.
...
