Path: Home > SN/T > Page73 > SN/T 3277-2012

Price & Delivery

US$319.00 · In stock · Download in 9 seconds
SN/T 3277-2012: Detection and identification of avocado sunblotch viroid
Delivery: 9 seconds. True-PDF full-copy in English & invoice will be downloaded + auto-delivered via email. See step-by-step procedure
Status: Valid

Std ID	Version	USD	Buy	Deliver [PDF] in	Title (Description)
SN/T 3277-2012	English	319	Add to Cart	3 days [Need to translate]	Detection and identification of avocado sunblotch viroid

SN/T 3277-2012: Detection and identification of avocado sunblotch viroid

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of avocado sunblotch viroid People's Republic of China Entry-Exit Inspection and Quarantine Standards Avocado day quarantine and identification of plaque-like virus Issued on. 2012-10-23 2013-05-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China, Shenzhen CIQ, Shenzhen Inspection and Quarantine Science Research Institute, China Inspection Quarantine Science Research Institute. The main drafters of this standard. Zheng Yun, Chen Chi Nan, Zhang Yongjiang, rain Lu, Feng Jianjun, Wang Ying, Li Yinong, Liu Jiao. Avocado day quarantine and identification of plaque-like virus

1 Scope

This standard specifies the entry plant quarantine for avocado sunspot-like virus RT-PCR, and from the polyacrylamide gel electrophoresis and biology Three quarantinable identification method for the determination. This standard applies to the entry and exit inspection and identification of avocado avocado seed in sunspot-like virus.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. SN/T 2122 entry and exit quarantine of plants and plant products sampling rules Principle 3 Avocado sunspot-like virus (avocadosunblotchviroid, ASBVd) belongs to the virus family avocado date of grouper (Avsunviroidae) avocado Sunspot-like virus genus (Avsunviroid). Biology and molecular biological properties such virus (see Appendix A) is a major quarantine and identification in accordance with. 4 equipment and facilities, equipment and reagents 4.1 Equipment and Facilities Balance (a sense of volume, 1/10000g), pH meter, PCR instrument, electrophoresis, electrophoresis tank, gel imaging system, quarantine greenhouses, water bath, Cryogenic refrigerators. Micropipette (2μL, 10μL, 20μL, 100μL, 200μL, 1000μL), mortar tubes, flower pots, sterilize soil. 4.2 RT-PCR detection reagent See Appendix B. And from 4.3 polyacrylamide gel electrophoresis detection reagents See Appendix C.

5 Sample Preparation

Sampling carried out in accordance SN/T 2122's. 5.1 seeds Random sample of 100 seeds, divided into five groups, at (20 ℃ ~ 28 ℃) conditions for germination in a suitable germination temperature until the first grow Pair of true leaves. 20 seedlings were randomly selected leaves per plant take 1g blade to prepare a sample. ...