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Detection and identification of Leptosphaeria lindquistii Frezzi
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Basic data
| Standard ID | SN/T 3174-2012 (SN/T3174-2012) |
| Description (Translated English) | Detection and identification of Leptosphaeria lindquistii Frezzi |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Classification of Chinese Standard | B16 |
| Classification of International Standard | 07.080 |
| Word Count Estimation | 16,190 |
| Regulation (derived from) | National Quality Inspection (2012) 237; industry standard filing Notice 2012 No. 9 (Total No. 153); |
| Issuing agency(ies) | General Administration of Customs |
| Summary | This standard specifies the black sunflower stem pathogen (Leptosphaeria lindquistii Frezzi) (see Appendix A) were isolated and cultured, pathogenicity test, sequence alignment of the experimental procedures and identification methods. This standard appli |
SN/T 3174-2012: Detection and identification of Leptosphaeria lindquistii Frezzi
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Detection and identification of Leptosphaeria lindquistii Frezzi
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Sunflower black stem quarantine and identification of bacteria
DetectionandidentificationofLeptosphaerialindquisti Frezzi
Issued on. 2012-05-07
2012-11-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of the content of this document may involve patents. Distribution of this document
Institutions do not assume the responsibility to identify these patents.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Tianjin People's Republic of China Entry-Exit Inspection and Quarantine Bureau, Chinese Academy of Inspection and Quarantine.
The main drafters of this standard. Roca phoenix, Liuyue Ting, Wu Shan goods, Liu Fang, Liu Peng, Zhang Yujun.
Sunflower black stem quarantine and identification of bacteria
1 Scope
This standard specifies the sunflower black stem germs (LeptosphaerialindquistiiFrezzi) (see Appendix A) Isolation, pathogenicity
Determination of the experimental step sequence alignment and identification methods.
This standard applies to sunflower seeds Sunflower black stem quarantine and identification of bacteria and germs sunflower in black stem Sunflower Field
Testing.
2 Sunflower black stem germs basic information
Name. LeptosphaerialindquistiiFrezzi
Asexual generation scientific name. PhomamacdonaldiiBoerma.
English name. SunflowerPhomablackstem
Taxonomic position. Sunflower black stem bacteria fungi industry (Fungi), Ascomycota (Ascomycota), chamber Proteobacteria
(Leculoascomycetes), Alternaria fungus cavity mesh (Pleosporales), Alternaria fungus chamber Branch (Pleosporaceae), the ball chamber genus (Lepto-
sphaeria); anamorph belongs mitosis fungal spores (Sphaeropsidales), Coelomycetes (Coelomycetes), Sphaeropsidales (Spha-
eropsidales), Phoma (Phoma).
3 PRINCIPLE OF THE METHOD
Pathogen isolation and culture traits, bacteria morphology, host of the pathogenicity of symptoms (see Appendix B) and bacteria ITS1 /
ITS4 and ActF1/R1 sequence alignment as a result of quarantine and identification based on sunflower black stem bacteria.
4 Equipment and Reagents
4.1 Equipment
Sterile Petri dish (diameter 90mm ~ 100mm), flask, forceps, surgical scissors, scalpels, inoculation needles, disposable syringes
(1mL), cotton yarn, cotton, alcohol lamp, plastic pestle, adjustable micro pipette.
Stereo microscope, biological microscope, clean benches, incubators (24 ℃ ± 1 ℃), electronic balance (inductance 0.01g), autoclaving
Pot, conventional refrigerator (-20 ℃), PCR amplification device, electrophoresis, gel imager, high-speed refrigerated centrifuge, heated water bath.
4.2 Reagents and culture medium
Ethylene diamine tetra-acetic acid (EDTA), sodium dodecyl sulfate (SDS), Tris-HCl, iso-amyl alcohol, chloroform, ethanol, potassium chloride,
Magnesium, proteinase K, primers ITS1/ITS4 and ActF1/R1, Taq polymerase and other molecular biology reagents. Or plant tissue and fungal groups
Extraction Kit genomic DNA. Potato, dextrose, agar, penicillin, streptomycin, lactic acid, 1.0% sodium hypochlorite (NaClO).
Acidic medium potato APDA (pH4.5 ~ 5.0), APDA selective medium (see Appendix C).
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