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Identification of Fusarium oxysporum f.sp. cubense
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Basic data
| Standard ID | SN/T 2665-2010 (SN/T2665-2010) |
| Description (Translated English) | Identification of Fusarium oxysporum f.sp. cubense |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Classification of Chinese Standard | B16 |
| Classification of International Standard | 07.080 |
| Word Count Estimation | 10,122 |
| Date of Issue | 2010-11-01 |
| Date of Implementation | 2011-05-01 |
| Quoted Standard | SN/T 1538.1 |
| Regulation (derived from) | National Quality Inspection (2010) 582 |
| Issuing agency(ies) | General Administration of Customs |
| Summary | This standard specifies requirements for entry and exit of banana seedlings, tissue culture and other banana germplasm resources, commodities, etc. with banana banana fusarium wilt (Fusarium oxysporum f. sp. cubense) quarantine and identification methods. This standard applies to all entry and exit of banana seedlings, tissue culture and other banana germplasm, bananas and other goods used in Fusarium quarantine. |
SN/T 2665-2010: Identification of Fusarium oxysporum f.sp. cubense
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Identification of Fusarium oxysporum f.sp.cubense
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Quarantine and identification of Fusarium oxysporum f
Issued on. 2010-11-01
2011-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Guangdong CIQ.
The main drafters of this standard. Chending Hu, Yang Lei Liang, Luo Lijuan, Wang Zhang roots, Qiu Deyi, Yun-Hua Wang, pipe dimensions.
Quarantine and identification of Fusarium oxysporum f
1 Scope
This standard provides for the entry and exit of banana seedlings, tissue culture banana germplasm and other commodity like banana Fusarium oxysporum
(Fusariumoxysporumf.sp.cubense) quarantine and identification methods.
This standard applies to all entry and exit banana seedlings, tissue culture and other banana germplasm, bananas and other goods with the Fusarium oxysporum
Quarantine.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
SN/T 1538.1 media preparation Guide - Part 1. General laboratory quality assurance prepared medium
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
PDA medium PDAmedium
Potato dextrose agar medium, for separating phytopathogenic fungi.
3.2
YPD broth YPDliquidmedium
Yeast peptone glucose culture medium for culturing Fusarium oxysporum f produce spores.
3.3
Chlamydospore chlamydospore
Individual cells are formed by the swollen hyphae, with a thick and concentrated protoplasm, resistant to heat, cold, drought, poor nutrition shortages ring
Environmental conditions a dormant spore.
Principle 4
Banana banana wilt disease, also known as Panama disease, yellow leaf disease, destruction of vascular plants led to the death of a devastating disease, its pathogen tip
Fusarium Cuba biotypes, the English called BananaFusarium Wilt, Bananabanamadisease, scientific name Fusarium
oxysporumf.sp.Cubense. Belonging to imperfect fungi Amon (Deuteromycotina), Trichosporon Gang (Hyphomycetes), tumor seat spore head
(Tuberculariales).
Hazard symptoms of the pathogen, morphological characteristics of colonies, pathogenicity and host range of the measurement results is based on the identification of the bacteria.
5 equipment and reagents
5.1 Equipment
Electronic balance (inductance 1/10000g), clean table, autoclaves, cryogenic refrigerators, water, adjustable micro pipette (2μL ~
...