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Detection and identification of rice gall dwarf virus
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Basic data
| Standard ID | SN/T 2635-2010 (SN/T2635-2010) |
| Description (Translated English) | Detection and identification of rice gall dwarf virus |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Classification of Chinese Standard | B16 |
| Classification of International Standard | 07.080 |
| Word Count Estimation | 8,816 |
| Date of Issue | 2010-05-27 |
| Date of Implementation | 2010-12-01 |
| Regulation (derived from) | National Quality Inspection (2010) 290; industry standard filing Notice 2010 No. 10 (No. 130 overall) |
| Issuing agency(ies) | General Administration of Customs |
| Summary | This standard specifies the identification of rice gall dwarf virus quarantine procedures and methods. This standard applies to Rice Rice gall dwarf virus is detected. |
SN/T 2635-2010: Detection and identification of rice gall dwarf virus
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of rice gall dwarf virus
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Quarantine and identification of rice gall dwarf virus
Issued on. 2010-05-27
2010-12-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Fujian Entry-Exit Inspection and Quarantine Xiamen People's Republic of China
Bureau, Fujian Agriculture and Forestry University, Chinese Academy of Inspection and Quarantine.
The main drafters of this standard. Shen Jianguo, Liao Furong, Gao Fang Luan, water Zhu Fang, GUO Qiong-xia, Wangnian Wu, Yu Yun, Huang Hui, Zu Jian.
Quarantine and identification of rice gall dwarf virus
1 Scope
This standard specifies the rice gall dwarf virus quarantine procedures and identification methods.
This standard applies to the detection of Rice gall dwarf virus on rice.
Principle 2
Rice gall dwarf virus Scientific name. ricegaldwarfvirus, Abbreviation. RGDV. Rice gall dwarf virus molecular characterization of this standard
The main basis for the development of; the taxonomic status of the virus, the host range, disease symptoms, geographical distribution, transmission, particle morphology, the viral genome
Etc. See Appendix A.
3 equipment, appliances, and reagents
3.1 Equipment
High-speed refrigerated centrifuge, microbalance (1/1000g), PCR, quantitative fluorescent PCR, electrophoresis, horizontal electrophoresis tank, gel imaging
Analysis system, pH meter, a microwave, a magnetic stirrer, constant temperature water bath, autoclaves, clean benches and the like.
3.2 Appliances
Various measurement ranges adjustable pipette (1000μL, 200μL, 100μL, 20μL, 10μL, 2μL), PCR reaction tube, RNase-free from
Heart tube (1.5mL), mortar and the like.
3.3 Reagents
3.3.1 TrizoL lysate.
3.3.2 5 × TBE buffer
54.0g Tris base
Boric acid 27.5g
0.5mol/LEDTA (pH8.0) 20mL
Add distilled water to 1L.
3.3.3 6 × loading buffer
0.25% bromophenol blue
40% (mass concentration) aqueous solution of sucrose.
3.3.4 chloroform.
3.3.5 isopropanol.
3.3.6 75% ethanol.
3.3.7 ethanol.
3.3.8 RT buffer.
3.3.9 dNTP. 10mmol/L.
...
