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SN/T 2615-2010 PDF English

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SN/T 2615-2010: Detection and identification of Phialophora malorum (Kidd and Beaumont) McColloch
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SN/T 2615-2010English239 Add to Cart 3 days [Need to translate] Detection and identification of Phialophora malorum (Kidd and Beaumont) McColloch

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Basic data

Standard ID SN/T 2615-2010 (SN/T2615-2010)
Description (Translated English) Detection and identification of Phialophora malorum (Kidd and Beaumont) McColloch
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B16
Classification of International Standard 07.080
Word Count Estimation 9,974
Date of Issue 2010-05-27
Date of Implementation 2010-12-01
Regulation (derived from) National Quality Inspection (2010) 290; industry standard filing Notice 2010 No. 10 (No. 130 overall)
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the Apple side rot phytosanitary quarantine and identification of bacteria. This standard applies to apples and pears into the exit side of apple fruit rot quarantine and identification.

SN/T 2615-2010: Detection and identification of Phialophora malorum (Kidd and Beaumont) McColloch




---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Phialophora malorum (Kidd People's Republic of China Entry-Exit Inspection and Quarantine Standards Apple side Rot quarantine and identification methods DetectionandidentificationofPhialophoramalorum (Kidd McColoch Issued on. 2010-05-27 2010-12-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. China Inspection and Quarantine Science Research Institute, Xinjiang People's Republic of China Exit Inspection and Quarantine, the People's Republic of China Zhejiang Exit Inspection and Quarantine and the State. Drafters of this standard. Wu Shan goods, Du Hongzhong, Wang Wen-cheng, Wu Zhiyi, Yan Jin. Apple side Rot quarantine and identification methods

1 Scope

This standard specifies the identification of phytosanitary quarantine in Apple's side Rot. This standard applies to entry and exit quarantine and identification of apples and pears apples rot bacteria edge. Principle 2 Apple side Rot symptoms in the host, morphology and molecular biological characteristics of the main basis for the development of this standard.

3 apparatus and appliances

3.1 Instrument Microscope (with measurements), stereo microscope, clean benches, light biological incubator, scales, autoclaves, high-speed desktop Centrifuges, pH meter, water bath, PCR amplification device, electrophoresis, gel imager. 3.2 Appliances Beakers, flasks, graduated cylinder, test tubes, Petri dishes (diameter 9cm), slides, cover slips, alcohol lamp, plastic pestle, pipettes, pipette Pipette tips, centrifuge tubes, liquid nitrogen tank.

4 Reagents and culture medium

4.1 Reagents Agar, potato, glucose, lactate, agarose, sterile double distilled water, liquid nitrogen, Tris-HCl, magnesium oxide, hydrochloric acid, EDTA, hydroxide Sodium acetate, sodium chloride, potassium chloride, Tris, CTAB, TAE, ethanol, phenol, ethidium bromide (EB), chloroform, isopropanol, isoamyl alcohol, protein Enzyme K, TaqDNA polymerase, dNTP, DNA molecular weight Marker, PCR primers PMA-1 and PMA-2, real-time fluorescence PCR primers PMA-P1, PMA-P2 and probe PMA-Probe-457. 4.2 Medium Potato dextrose medium (PDA). potato 200g, dextrose 20g, Agar 20g, Distilled water 1000mL, sterilization. Acidic potato dextrose medium (APDA). Preparation 1000mLPDA, after sterilization was cooled to 50 ℃, added 1.5mL85% Lactic acid.

5 identification methods

5.1 Symptom Check Disease symptoms in Appendix A. ...

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