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SN/T 2600-2010: Methods for rapid identification of Metasequoia glptostroboides genetic resources for export
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SN/T 2600-2010: Methods for rapid identification of Metasequoia glptostroboides genetic resources for export

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Methods for rapid identification of Metasequoia glptostroboides genetic resources for export People's Republic of China Entry-Exit Inspection and Quarantine Standards Outbound Metasequoia germplasm resources for rapid identification method Issued on. 2010-05-27 2010-12-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard is drafted by. People's Republic of China Hubei Exit Inspection and Quarantine. Participated in the drafting of this standard. Hubei Academy of Forestry. The main drafters of this standard. Li Jinfu, three mountain Cai, Wang Zhenhua, Chen Jing Yuan, Qiu Guoqiang, Jin Yong.

Introduction

Rare species of plant genetic nature left us a valuable asset, but also a scarce resource for the world of competition. Entry-Exit Inspection and Phytophthora species as a biological resource agency inbound and outbound inspection of law enforcement agencies, the urgent need for accurate and rapid species identification techniques to improve port Check the efficiency of the work to ensure the protection of biological species resources in China. This standard molecular biology techniques, through the collection and laboratory testing to verify the information, the establishment of a national plant protection Was Metasequoia (Metasequoiaglyptostroboides) accurate and rapid identification methods, to solve the key techniques for the implementation of port inspection of Metasequoia Problems, improve the efficiency of the inspection, effectively prevent the loss of Metasequoia germplasm resources. Outbound Metasequoia germplasm resources for rapid identification method

1 Scope

This standard specifies the identification methods Metasequoia (Metasequoiaglyptostroboides) germplasm resources. This standard applies to when to exit Metasequoia germplasm resources inspection, their identification. 2 terms, definitions and abbreviations 2.1 Terms and Definitions The following terms and definitions apply to this document. 2.1.1 Internal transcribed spacer internaltranscribedspacer, ITS Plant cell nucleus, the gene encoding rRNA is a multigene family consisting of a number of highly repetitive sequences, wherein the encoding ribosomal small subunit The 18S rRNA gene group and 5.8S, 26S together constitute a gene transcription unit (see FIG. 1). Wherein the base 5.8S and 18S, 26S between Inter were due to area internal transcribed spacer (ITS) 1 and 2. That is, ITS 5.8S region is divided into two regions ITS1 and ITS2. ITS1 And ITS2 transcript was cut off during the processing of rRNA, but the two parts has an important role in the ripening process of nuclear rRNA.

1 ITS area chart

2.1.2 Polymerase chain reaction polymerasechainreaction, PCR Template DNA sequence variability at high temperature into a single strand, DNA polymerase and under appropriate reaction conditions, according to the template DNA The sequences of two primers designed with respective complementary sequences annealing takes place on a section of the template DNA combined with each other and both strands, followed by The role of DNA polymerase in four DNA (dNTP) as substrates, primer can be extended, and then repeated denaturation, annealing And extension of the cycle, so that the amplified gene fragment want to geometrically amplify. 2.2 Acronyms The following abbreviations apply to this document. 2.2.1 DNA deoxyribonucleicacid DNA. 2.2.2 dNTP deoxyribonucleosidetriphosphate Deoxynucleotide triphosphates. 2.2.3 dATP deoxyadenosinetriphosphate Triphosphate. ...