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JJG 537-2006: Verification Regulation of Fluorescence Spectrophotometer
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Std ID	Version	USD	Buy	Deliver [PDF] in	Title (Description)
JJG 537-2006	English	519	Add to Cart	4 days [Need to translate]	Verification Regulation of Fluorescence Spectrophotometer
JJG 537-1988	English	559	Add to Cart	4 days [Need to translate]	Verification Regulation of Fluorescence Spectro Photometer

JJG 537-2006: Verification Regulation of Fluorescence Spectrophotometer

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Verification Regulation of Fluorescence Spectrophotometer National Metrology Verification Regulations of the People's Republic Fluorescence spectrophotometer Released on.2006-05-23 Implementation of.2006-11-23 The General Administration of Quality Supervision, Inspection and Quarantine issued Fluorescence spectrophotometer verification procedure Replace JJG 537-1988 JJG 538-1988 This regulation was approved by the General Administration of Quality Supervision, Inspection and Quarantine on May 23,.2006, and Implemented on November 23,.2006. Focal Point. National Physical and Chemical Metrology Technical Committee Main drafting unit. China Institute of Metrology Participated in drafting unit. Shanghai Sanke Instrument Co., Ltd. This procedure entrusts the National Physical and Chemical Metrology Technical Committee to explain The main drafters of this procedure. Cao Wenzhao (China Institute of Metrology) He Yajuan (China Institute of Metrology) Participate in the drafters. Huang Zhengyi (Shanghai Sanke Instrument Co., Ltd.) He Wei (Shanghai Sanke Instrument Co., Ltd.)

table of Contents

1 Scope (1) 2 Overview (1) 3 Metrological performance requirements (1) 3.1 Wavelength indication error and repeatability (1) 3.2 Detection limit (2) 3.3 Measurement linearity (2) 3.4 Fluorescence spectrum peak intensity repeatability (2) 3.5 Stability (2) 4 General technical requirements (2) 4.1 Appearance and preliminary inspection (2) 4.2 Insulation resistance (2) 5 measuring instrument control (2) 5.1 Verification conditions (2) 5.2 Verification project (3) 5.3 Verification method (3) 5.4 Processing of verification results (7) 5.5 Verification cycle (7) Appendix A Preparation of working standard solution for verification (8) Appendix B Measurement method of mercury lamp line (10) Appendix C Measurement of Fluorescent Pool Completeness (11) Appendix D Least Squares Calculation Formula (12) Appendix E Fluorescence Spectrophotometer Verification Record Format (13) Appendix F Verification Certificate Back Format (15) Fluorescence spectrophotometer verification procedure

1 Scope

This procedure applies to the first verification, subsequent verification and in-use inspection of the fluorescence spectrophotometer.

2 Overview

When some substances are excited by light, they emit light that is slightly longer than the wavelength of the light. This light is called fluorescing. Light. The fluorescence intensity has the following relationship with the concentration of the substance. F=k'ΦI0(1-e-εLc) Where. F---fluorescence intensity; K'---instrument constant; I0---excitation light intensity; ε---the molar absorption coefficient of the fluorescent substance; L---the thickness of the liquid layer of the fluorescent substance; c---concentration of the fluorescent substance; Φ---fluorescence quantum efficiency. For a given substance, when the wavelength and intensity of the excitation light are fixed, the thickness of the liquid layer is fixed, and the concentration of the solution is higher. When low, the fluorescence intensity has a simple relationship with the concentration c of the fluorescent substance. F=kc Fluorescence spectrophotometer (hereinafter referred to as the instrument) is based on the above principle, to determine the substance that can emit fluorescence Analytical instrument for qualitative and quantitative measurement. The instrument is mainly composed of a light source system, an excitation monochromator system, a sample chamber, a launch monochromator system, a detector system, and The data processing system is composed. The monochromators of the instrument can be divided into two categories. Class A is a dispersive monochromator; Class B is a filter monochromator.

3 Metrological performance requirements

3.1 Wavelength indication error and repeatability 3.1.1 Class A monochromator wavelength indication error limits and wavelength repeatability shall meet the following requirements. Wavelength indication error limit. better than ±2.0nm; Wavelength repeatability. ≤ 1.0 nm. 3.1.2 Class B Monochromator Filter Light Transmission Characteristics The nominal value of the glass filter and interference filter of the class B monochromator configuration shall meet the following requirements. Glass filter. nominal value ± 10 nm; ...