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Calibration Specification for Micro-spectrophotometers
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Basic data
| Standard ID | JJF 1836-2020 (JJF1836-2020) |
| Description (Translated English) | Calibration Specification for Micro-spectrophotometers |
| Sector / Industry | Metrology & Measurement Industry Standard |
| Classification of Chinese Standard | A60 |
| Word Count Estimation | 12,172 |
| Date of Issue | 2020-07-02 |
| Date of Implementation | 2021-01-02 |
| Regulation (derived from) | Announcement No. 29 (2020) of the State Administration for Market Regulation |
| Issuing agency(ies) | State Administration for Market Regulation |
JJF 1836-2020: Calibration Specification for Micro-spectrophotometers
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Calibration Specification for Micro-spectrophotometers
National Metrology Technical Specifications of the People's Republic of China
Calibration specification for micro spectrophotometer
2020-07-02 released
2021-01-02 implementation
Issued by the State Administration for Market Regulation
Calibration specification for micro spectrophotometer
Focal point. National Biometrics Technical Committee
Main drafting unit. China Institute of Metrology
Shanghai Institute of Metrology and Testing Technology
Boao Biological Group Co., Ltd.
This specification entrusts the National Biometrics Technical Committee to be responsible for the interpretation
The main drafters of this specification.
Gao Yunhua (China Institute of Metrology)
Xu Li (Shanghai Institute of Metrology and Testing Technology)
Wu Liqing (China Institute of Metrology)
Wang Dong (Boao Biological Group Co., Ltd.)
Liang Wen (Shanghai Institute of Metrology and Testing Technology)
Li Baolian (Boao Biological Group Co., Ltd.)
table of Contents
Introduction (Ⅱ)
1 scope (1)
2 Reference documents (1)
3 Overview (1)
4 Measurement characteristics (1)
5 Calibration conditions (2)
5.1 Environmental conditions (2)
5.2 Standard substances and reagents for calibration (2)
6 Calibration items and calibration methods (2)
6.1 Indication error (2)
6.2 Repeatability (2)
6.3 Linearity and linear range(3)
7 Calibration result expression (3)
8 Recalibration interval (3)
Appendix A Uncertainty Evaluation Example of Indication Error (4)
Appendix B Reference Format of Calibration Original Record (6)
Appendix C Reference Format of Calibration Certificate (Inner Page) (7)
Introduction
This specification is compiled in accordance with the requirements of JJF 1071-2010 "Rules for the Compilation of National Metrology and Calibration Specifications".
JJG178-2007 "Verification Regulations for Ultraviolet, Visible and Near Infrared Spectrophotometers", GB/T 26810-2011
"Visible Spectrophotometer".
This specification is released for the first time.
Calibration specification for micro spectrophotometer
1 Scope
This specification applies to the calibration of micro-spectrophotometers. For other types of spectrophotometers, refer to this specification.
2 Reference documents
This specification refers to the following documents.
JJG178-2007 Verification Regulations for Ultraviolet, Visible and Near Infrared Spectrophotometer
GB/T 26810-2011 Visible spectrophotometer
For dated reference documents, only the dated version applies to this specification; for undated references
The latest version (including all amendments) is applicable to this specification.
3 overview
The volume of the micro spectrophotometer is small and the sample amount is small [(1~2)μL]. It is composed of light source, monochromator, absorption cell,
The detector and signal indicating system consist of five parts. The principle is based on the selective absorption of light of different wavelengths and
Lambert-Beer (Lambert-Beer) law for quantitative analysis and qualitative identification of substances, commonly used in nucleic acids and proteins
Qualitative and quantitative analysis and quantification of bacterial concentration.
The mathematical expression of Lambert-Beer law is.
When testing, use a pipette to drop (1~2) μL of the sample onto the testing platform, and the instrument will automatically complete the test and display
Shows the absorbance value and gives the qualitative and quantitative results of the sample (nucleic acid, protein and fluorescent dye).
4 Measurement characteristics
4.1 Indication error
4.2 Repeatability
4.3 Linearity and linear range
...
