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Calibration Specification for McFarland Bacterial Turbidity Analyzers
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Basic data
| Standard ID | JJF 1825-2020 (JJF1825-2020) |
| Description (Translated English) | Calibration Specification for McFarland Bacterial Turbidity Analyzers |
| Sector / Industry | Metrology & Measurement Industry Standard |
| Classification of Chinese Standard | A60 |
| Classification of International Standard | 17.180 |
| Word Count Estimation | 13,129 |
| Date of Issue | 2020 |
| Date of Implementation | 2020-04-17 |
| Issuing agency(ies) | State Administration for Market Regulation |
JJF 1825-2020: Calibration Specification for McFarland Bacterial Turbidity Analyzers
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Calibration Specification for McFarland Bacterial Turbidity Analyzers
National Measurement Technical Specification of the People's Republic of China
Calibration Specification of McBee Turbidity Analyzer
2020-01-17 release
2020-04-17 Implementation
Issued by the State Administration of Market Supervision and Administration
Calibration Specification of McBee Turbidity Analyzer
Focal point. National Biometrics Technical Committee
Main drafting unit. Shanghai Institute of Metrology and Testing Technology
Participated in the drafting unit. China Academy of Metrology
This specification entrusts the National Biometrics Technical Committee to interpret
The main drafters of this specification.
Wen Yanli (Shanghai Institute of Measurement and Testing Technology)
Xu Li (Shanghai Institute of Metrology and Testing Technology)
Gang Liu (Shanghai Institute of Metrology and Testing Technology)
Participating drafters.
Sui Zhiwei (Chinese Academy of Metrology)
table of Contents
Introduction (Ⅱ)
1 Scope (1)
2 Terms and units of measurement (1)
2.1 McNeil Turbidimetry (1)
2.2 Meyer's unit (1)
3 Overview (1)
4 Measurement characteristics (1)
4.1 Indication stability (1)
4.2 Repeatability (1)
4.3 Indication error (1)
5 Calibration conditions (1)
5.1 Environmental conditions (1)
5.2 Reference Standards for Calibration (1)
6 Calibration items and calibration methods (1)
6.1 Indication stability (1)
6.2 Repeatability (2)
6.3 Indication error (2)
7 Expression of calibration results (2)
8 Recalibration time interval (3)
Appendix A Examples of Uncertainty Evaluation of Indication Error Measurement Results (4)
Appendix B. Reference Format for Original Calibration Records (6)
Appendix C Calibration Certificate (Inner Page) Reference Format (8)
Introduction
In drafting this specification, reference was made to JJG880-2006 "Turbidity Meter", JJF 1059.1-2012 "Measurement Uncertainty
"Assessment and Representation", prepared in accordance with the requirements of JJF 1071-2010 "Rules for Compiling National Measurement and Calibration Specifications".
This specification is the first release.
Calibration Specification of McBee Turbidity Analyzer
1 Scope
This specification applies to the measurement performance of Bacterial Turbidity Analyzer (BacterialTurbidityAnalyzer)
calibration.
2 Terminology and units of measurement
2.1 McFarlandmethod
The method of estimating the number of bacteria by measuring the light transmission (turbidity value) of the bacterial suspension, the concentration of the bacterial suspension is
Within a certain range, it is inversely proportional to the light transmittance and directly proportional to the optical density.
2.2 McFarlandunit
The unit of bacterial turbidity measured according to the McNeil turbidimetry is MCF.
3 Overview
The McNair bacterial turbidity analyzer (hereinafter referred to as the instrument) is a method for detecting the scattered light of microorganisms in suspension
An instrument that reflects the number of microorganisms. The principle is that the greater the content of microorganisms in the suspension, the less light is transmitted through the suspension,
The more light is scattered. It is mainly used for bacterial turbidity in the fields of drug sensitivity evaluation, microbial fermentation and microbial detection
Detection.
4 Metering characteristics
4.1 Indication stability
4.2 Repeatability
4.3 Indication error
5 Calibration conditions
5.1 Environmental conditions
The operating temperature does not exceed 30 ° C, and the relative humidity ranges from 40% to 80%. During the measurement, the ring should be measured and recorded.
Environment temperature and humidity.
5.2 Standard materials for calibration
Bacterial turbidity certified reference materials should be used during calibration.
0.5MCF, 1.0MCF, 2.0MCF, 3.0MCF, 4.0MCF), relative expansion uncertainty ≤10%
(k = 2).
6 Calibration items and calibration methods
6.1 Indication stability
According to the requirements of the instrument manual, if zero adjustment is required, use a bacterial turbidity standard material with a nominal value of 0.0MCF
...
