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JJF 1819-2020: Calibration Specification for Medical Micro-image Analyzers
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JJF 1819-2020: Calibration Specification for Medical Micro-image Analyzers

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(Calibration specification for medical microscopic image measurement analyzer) National Metrology Technical Specifications of the People's Republic of China Calibration specification for medical microscopic image measurement analyzer 2020-01-17 released 2020-04-17 implementation Issued by the State Administration for Market Regulation Medical microscopic image measurement analyzer Calibration specification Focal point. National Medical Metrology Technical Committee Drafting unit. China Institute of Metrology Peking University Medical and Health Analysis Center This specification entrusts the National Medical Metrology Technical Committee to interpret Drafters of this specification. Liu Zilong (China Institute of Metrology) Yang Jianru (Peking University Medical and Health Analysis Center) Li Fei (China Institute of Metrology) Yuan Lan (Peking University Medical and Health Analysis Center) Zhang Pu (China Institute of Metrology) Jiang Yiqin (China Institute of Metrology) Li Yuxiao (China Institute of Metrology)

table of Contents

Introduction (Ⅱ) 1 scope (1) 2 Reference documents (1) 3 Term (1) 3.1 Optical density(1) 3.2 Grayscale repeatability (1) 3.3 Gray uniformity (1) 3.4 The dual-system magnification difference of the binocular microscope system (2) 3.5 Center deviation of dual field of view of binocular microscope system (2) 4 Overview (2) 5 Metering characteristics (3) 5.1 Optical density (3) 5.2 Grayscale repeatability (3) 5.3 Gray uniformity (3) 5.4 Magnification error of microscope objective lens (3) 5.5 The magnification difference of the left and right systems of the binocular microscope system (3) 5.6 Center deviation of left and right field of view of binocular microscope system (3) 6 Calibration conditions (3) 6.1 Environmental conditions (3) 6.2 Calibration equipment (3) 7 Calibration items and calibration methods (4) 7.1 Appearance (4) 7.2 Preparation before calibration (4) 7.3 Optical density(4) 7.4 Grayscale repeatability of the instrument (4) 7.5 Gray uniformity of the instrument (4) 7.6 Magnification deviation of microscope objective lens (4) 7.7 The magnification difference of the left and right systems of the binocular microscope system (5) 7.8 Center deviation of left and right field of view of binocular microscope system (6) 7.9 Matters needing attention (6) 8 Calibration result expression (6) 8.1 Calibration record (6) 8.2 Processing of calibration results (6) 9 Re-calibration interval (7) Appendix A Original Record (Recommended) Format of Calibration of Medical Microscopic Image Measurement Analyzer (8) Appendix B. Internal page of calibration certificate (recommended) format (9) Appendix C Example of Uncertainty Evaluation of Optical Density Calibration (11)

Introduction

JJF 1071-2010 "Rules for the Compilation of National Metrology and Calibration Specifications", JJF 1001-2011 "General Metrology Terms and Definitions", JJF 1032-2005 "Terms and Definitions of Optical Radiation Measurement" and JJF 1059.1-2012 "Inaccurate Measurement "Fixed Evaluation and Representation" together constitute a basic series of specifications supporting the formulation of this specification. This specification is released for the first time. Calibration specification for medical microscopic image measurement analyzer

1 Scope

This specification specifies the metrological characteristics, calibration conditions, and calibration conditions of medical microscopic image measurement analyzers applicable to tissue sections. The calibration method is suitable for the measurement performance calibration of microscopic image measurement analyzers newly manufactured, in use and after repair. its The microscopic image measurement analyzer used in other industries can refer to this specification.

2 Reference documents

This specification refers to the following documents. GB/T 9247-2008 microscope condenser GB/T 22056-2018 (ISO 8578.2012) Marking of microscope objectives and eyepieces JJG920-2017 Verification Regulations for Diffuse Transmission Visual Densitometer JJF 1402-2013 Calibration Specification for Biological Microscope For dated reference documents, only the dated version applies to this specification; for undated references The latest version (including all amendments) is applicable to this specification.

3 Term

3.1 Optical density, opticaldensity, D The reciprocal of the ratio of the signal intensity Iτ received by the detector (including the microscope lens) to the light source intensity Is is taken as the logarithm, the symbol For D. The definition is shown in formula (1). D=log10 Is Iτ (1) Optical density is the physical basis of the gray-scale characteristics of microscopic image analyzers. The use of optical density can achieve The measurement system including the source, microscope and its components, and detectors are calibrated. 3.2 Grayrepeatability, ΔG The change in the gray value of the entire image with gray level K. The K value is determined according to formula (2). K=Round [(75%~85%)×Km] (2) Where. Km-the maximum gray level. Calculate the gray scale repeatability ΔG of the instrument according to formula (3). ΔG = max(Gi- i=1 Gi ) (3) Where. Gi---Gray value of gray level K of a certain measurement. 3.3 Gray uniformity, U In the blank image output by the instrument with gray level K, select the measured value Gi of M×N sampling areas, and press ...