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GB/T 46506-2025: Code of practice for spawn production of Wolfiporia hoelen
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GB/T 46506-2025: Code of practice for spawn production of Wolfiporia hoelen

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ICS 65.020.20 CCSB39 National Standards of the People's Republic of China Technical Specifications for the Production of Poria Cocos Mycelium Published on 2025-10-05 Implemented on February 1, 2026 State Administration for Market Regulation The State Administration for Standardization issued a statement.

Table of Contents

Preface III 1.Scope 1 2 Normative References 1 3.Terms and Definitions 1 4.Production Process 2 5.Mother seed production 2 5.1 Site Selection 2 5.2 Facility and Equipment Configuration 2 5.3 Selection of microbial strains 3 5.4 Culture medium preparation 3 5.5 repackaged in 3 portions 5.6 Sterilization 3 5.7 Cooling 3 5.8 Vaccination 3 5.9 Cultivation 3 5.10 Inspection 3 5.11 Sample 4 5.12 Labelling and Packaging 4 5.13 Warehousing and Storage 4 6.Original seed production 4 6.1 Site Selection 4 6.2 Facility and Equipment Configuration 4 6.3 Selection of microbial strains 4 6.4 Culture medium preparation 4 6.5g repackaged in 4g portions 6.6 Sterilization 4 6.7 Cooling 5 6.8 Vaccination 5 6.9 Cultivate 5 6.10 Inspection 5 6.11 Sample 5 6.12 Labelling and Packaging 5 6.13 Warehousing and Storage 6 7.Cultivated seed production 6. 7.1 Site Selection 6 7.2 Facility and Equipment Configuration 6 7.3 Selection of microbial strains 6 7.4 Culture medium preparation 6 7.5 repackaged in 6 portions 7.6 Sterilization 6 7.7 Cooling 6 7.8 Vaccination 6 7.9 Cultivation 6 7.10 Inspection 7 7.11 Sample Retention 7 7.12 Labelling and Packaging 7 7.13 Warehousing and Storage 7 8 Waste disposal 7 9.Production records and file management 7. Appendix A (Normative) Facility and Equipment Configuration Requirements 8 A.1 Mother Seed Production 8 A.2 Original Seed Production 8 A.3 Cultivated Seed Production 8 Appendix B (Informative) Commonly Used Culture Media Raw Materials and Formulations 9 B.1 Mother Seed 9 B.2 Original Species 9 B.3 Cultivated Variety 9

Foreword

This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1.Structure and Drafting Rules of Standardization Documents". Drafting. Please note that some content in this document may involve patents. The issuing organization of this document assumes no responsibility for identifying patents. This document was proposed and is under the jurisdiction of the All China Federation of Supply and Marketing Cooperatives. This document was drafted by. Jingzhou Miao and Dong Autonomous County Poria Cocos Professional Association, Hunan Provincial Institute of Quality and Standardization, and Hunan Provincial Edible Fungi Association. Association, China Edible Fungi Association, Huaihua Municipal Market Supervision Administration, Jingzhou Zhimei Health Technology Co., Ltd., Hunan Provincial Edible Fungi Research Institute Institute, China National Institute of Standardization, Hunan Provincial Excellent Standards and Coding Technology Service Center, Jingzhou Huayi Poria Cocos Technology Co., Ltd., Jingzhou County Longxuan Spawn producers, Jingzhou Kangyuan Lingye Technology Co., Ltd., Yunnan Baiyao Group Seed Industry Technology Co., Ltd., Shaanxi Chunsen Fungi Industry Co., Ltd. Anhui University of Traditional Chinese Medicine, Pu'er Yulin Agricultural Technology Development Co., Ltd., Yunnan Miaorong Biotechnology Co., Ltd., Hunan Provincial Forestry Science Institute, Jingzhou Miao and Dong Autonomous County Quality and Metrology Inspection and Testing Center, Shaanxi Qingruifeng Edible and Medicinal Fungi Technology Co., Ltd., Jingzhou County Medicinal and Edible Fungi Research institute, Zhejiang Tianquan Biotechnology Co., Ltd., Fujian Lingshanyu Health Technology Co., Ltd., and the Agriculture and Rural Affairs Bureau of Jingzhou Miao and Dong Autonomous County. The main drafters of this document are. Wang Xianyou, Gong He, Peng Yunxiang, Peng Xiahua, Huang Xiaohui, Yin Xiaoqin, Hu Ruxiao, Chen Weidong, and Xu Zequn. Xi Xingjun, Yang Huan, Shi Lizhen, Yang Chengjin, Zhang Yue, Wang Lei, Wang Yanyan, Han Hongyi, Tan Zhuoming, Sun Yeguang, Mao Fang, Sun Hua, Gao Xuan, Yang Guofeng Zhen Dagui, Zhang Shengming, Song Yunhua, Chu Zhifan, Zheng Zhong, Yang Binghe, Yang Xinqing, Huang Lizhi, Zhao Peisong, Liang Jingwu, Huang Liping, Ni Xiaolin Dao Yongxiu. Technical Specifications for the Production of Poria Cocos Mycelium 1.Scope This document establishes the production procedures for Poria cocos strains, specifying the technical requirements and waste management for mother strain production, primary strain production, and cultivar production. The processing requirements describe the content of production record and file management. This document applies to the production of Poria cocos strains.

2 Normative references

The contents of the following documents, through normative references within the text, constitute essential provisions of this document. Dated citations are not included. For references to documents, only the version corresponding to that date applies to this document; for undated references, the latest version (including all amendments) applies. This document. GB 5749 Standard for Drinking Water Quality GB/T 12728 Terminology for Edible Fungi NY/T 528-2010 Technical Specifications for Edible Fungi Spawn Production 3.Terms and Definitions The terms and definitions defined in GB/T 12728 and the following terms and definitions apply to this document. 3.1 Hypha The structural unit of filamentous fungi. Note. Composed of tubular cells, multinucleated, septate, and forming the building blocks of mycelium. [Source. GB/T 12728-2006, 2.2.1, with modifications] 3.2 mycelium An aggregate of hyphae. [Source. GB/T 12728-2006, 2.2.2] 3.3 rhizomorph Rope-like structures formed by the aggregation of hyphae in certain fungi. [Source. GB/T 12728-2006, 2.2.12, with modifications] 3.4 Antagonism The phenomenon of colonies with different genetic makeup inhibiting each other, resulting in non-growing zones or forming different forms of linear edges. [Source. GB/T 12728-2006, 2.3.21] 3.5 Angular sectoring This phenomenon, caused by localized mutations in the mycelium or viral infection, results in thinner mycelia, slower growth, and angular abnormalities on the surface of the mycelium. [Source. GB/T 12728-2006, 2.5.17] ...