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Determination of hydroxymethyl furfural content in royal jelly and royal jelly lyophilized powder - High-performance liquid chromatography method
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Basic data
| Standard ID | GB/T 45625-2025 (GB/T45625-2025) |
| Description (Translated English) | Determination of hydroxymethyl furfural content in royal jelly and royal jelly lyophilized powder - High-performance liquid chromatography method |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B47 |
| Classification of International Standard | 65.140 |
| Word Count Estimation | 10,171 |
| Date of Issue | 2025-04-25 |
| Date of Implementation | 2025-08-01 |
| Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration |
GB/T 45625-2025: Determination of hydroxymethyl furfural content in royal jelly and royal jelly lyophilized powder - High-performance liquid chromatography method
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 65.140
CCSB47
National Standard of the People's Republic of China
Hydroxymethylfurfural in royal jelly and royal jelly freeze-dried powder
Determination of content by HPLC
Released on 2025-04-25
2025-08-01 Implementation
State Administration for Market Regulation
The National Standardization Administration issued
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting.
This document was proposed by the China Federation of Supply and Marketing Cooperatives.
This document is under the jurisdiction of the National Technical Committee on Standardization of Bee Products (SAC/TC601).
This document was drafted by. Hangzhou Biyutian Health Products Co., Ltd., Zhejiang University, China Bee Products Association, Nanjing Tianjia Testing Services Co., Ltd.
Company, Tonglu County Inspection and Testing Center, Zhejiang Provincial Food and Drug Inspection Institute, Hangzhou Tianchu Miyuan Health Products Co., Ltd., Hangzhou Hefengyuan Health Products Co., Ltd.
Jianpin Co., Ltd., Anhui Mizhiyuan Food Group Co., Ltd., Shandong Fengcai Health Industry Co., Ltd., Zhejiang Jiangshan Hengliang Bee Products Co., Ltd.
Co., Ltd., Zhejiang Jiangshan Healthy Bee Industry Co., Ltd., Shanghai Guanshengyuan Bee Products Co., Ltd., Yichun University, Huapu Science and Technology (Beijing) Co., Ltd.
Co., Ltd., Shaanxi Guofeng Health Industry Co., Ltd.
The main drafters of this document are. Zhou Ping, Yin Xin, Hu Fuliang, Li Shanshan, Xu Jinzhong, Tan Lirui, Liang Shijun, Li Yinghong, Wang Lei, Zhou Xinglian,
Wang Yan, Li Jiafen, Zhang Ping, Zheng Haoliang, Xu Hanbo, Wang Qian, Jia Yingjie, Guo Mengping, Chen Jing, and Chen Yun.
Introduction
The issuing agency of this document draws attention to the fact that when declaring compliance with this document, Chapters 4 to 10 may be involved in relation to royal jelly and its dry powder.
Use of patents related to methods for detecting hydroxymethylfurfural content.
The issuing organization of this document takes no position on the authenticity, validity and scope of this patent.
The patent holder has promised to the issuing agency of this document that he is willing to cooperate with any applicant under reasonable and non-discriminatory terms and conditions.
The patent holder's statement has been filed with the issuing authority of this document.
Get in touch with.
Name of patent holder. Hangzhou Biyutian Health Products Co., Ltd.
Address. Hangzhou Biyutian Health Products Co., Ltd., No. 18, Sangyuan Road, Tonglu County Economic Development Zone, Hangzhou City, Zhejiang Province.
Please note that in addition to the above patents, some of the contents of this document may still involve patents. The issuing agency of this document does not assume the responsibility for identifying patents.
responsibility.
Hydroxymethylfurfural in royal jelly and royal jelly freeze-dried powder
Determination of content by HPLC
1 Scope
This document describes the HPLC method for the determination of hydroxymethylfurfural in royal jelly and royal jelly freeze-dried powder.
This document is applicable to the determination of hydroxymethylfurfural content in royal jelly and royal jelly freeze-dried powder.
2 Normative references
The contents of the following documents constitute essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to
This document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
3 Terms and definitions
There are no terms or definitions that require definition in this document.
4 Principle
The sample was dissolved in ammonia solution, hydroxymethylfurfural was extracted with ethyl acetate, centrifuged, the supernatant was evaporated to dryness, ultrasonically dissolved, and filtered through a membrane.
Detection was by high performance liquid chromatography and quantification was by external standard method.
5 Reagents and Materials
Unless otherwise specified, only reagents of analytical grade and first-grade water conforming to GB/T 6682 were used in the analysis.
5.1 Reagents
5.1.1 Ammonia water (NH3·H2O). containing 25%~28% ammonia (NH3).
5.1.2 Ethyl acetate (CH3COOC2H5).
5.1.3 Phosphoric acid (H3PO4). chromatographically pure, with a content of not less than 85%.
5.1.4 Methanol (CH3OH). chromatographically pure.
5.1.5 Anhydrous sodium sulfate.
5.2 Reagent preparation
5.2.1 Phosphoric acid solution (0.4%, volume fraction). Take 4 mL of phosphoric acid (5.1.3) and dilute to 1000 mL with water.
5.2.2 Ammonia solution (1.5%, volume fraction). Take 1.5 mL of ammonia solution (5.1.1) and dilute to 100 mL with water.
5.2.3 Methanol solution (6%, volume fraction). Take 6 mL of methanol (5.1.4) and dilute to 100 mL with water.
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