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Detection and identification of Greeneria uvicola
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Basic data
| Standard ID | GB/T 45586-2025 (GB/T45586-2025) |
| Description (Translated English) | Detection and identification of Greeneria uvicola |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B16 |
| Classification of International Standard | 65.020.20 |
| Word Count Estimation | 14,132 |
| Date of Issue | 2025-04-25 |
| Date of Implementation | 2025-11-01 |
| Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration |
GB/T 45586-2025: Detection and identification of Greeneria uvicola (Berk. and M. A. Curtis) Punith.
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 65.020.20
CCSB16
National Standard of the People's Republic of China
Quarantine and Identification Methods for Grape Bitter Rot Pathogen
Released on 2025-04-25
2025-11-01 Implementation
State Administration for Market Regulation
The National Standardization Administration issued
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting.
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document is proposed and coordinated by the National Technical Committee on Plant Quarantine Standardization (SAC/TC271).
This document was drafted by. Ningbo Institute of Inspection and Quarantine Science and Technology (Ningbo National Inspection Trade Facilitation Service Center), China Inspection and Quarantine
Institute of Microbiology, Chinese Academy of Sciences, Institute of Plant Protection, Chinese Academy of Agricultural Sciences.
The main drafters of this document are Duan Weijun, Zhang Huili, Wu Pinshan, Cai Lei, Li Xuelian, Lei Rong, Zhao Peng, Gao Li and Chen Xianfeng.
Quarantine and Identification Methods for Grape Bitter Rot Pathogen
1 Scope
This document describes the bitter rot pathogen Greeneriauvicola (Berk.
Biological detection methods.
This document is applicable to the quarantine detection and identification of grape bitter rot pathogens in tissues such as leaves, stems and fruits of host plants.
2 Normative references
This document has no normative references.
3 Terms and definitions
There are no terms or definitions that require definition in this document.
4 Basic Information
Chinese name. Grape bitter rot.
Scientific name. Greeneriauvicola (Berk.
Classification. Fungi, Ascomycota, Pezizomycotina, Sordar-
iomycetes), Diaporthales, Melanconielaceae, Greeneria.
For distribution, host, and other information, see Appendix A.
5 Identification Principles
The symptoms of grape bitter rot pathogens in harming the host, the morphological characteristics of the pathogen and the molecular biological characteristics are the basis for identifying the pathogen.
6 Instruments and equipment
6.1 Biological microscope. with measurement function.
6.2 Dissecting microscope. minimum 10×.
6.3 Constant temperature and light incubator. 5℃~60℃, temperature accuracy ±0.1℃.
6.4 Electronic balance. sensitivity 0.001g.
6.5 Autoclave. Maximum pressure is 0.235MPa, and the adjustable temperature range is 105℃~135℃.
6.6 Desktop centrifuge. speed range is between 10000r/min and 30000r/min.
6.7 pH meter. range. pH 0.00~14.00, accuracy. ±0.01pH.
6.8 Constant temperature water bath. temperature range 4℃~100℃, temperature control accuracy ±0.3℃.
6.9 PCR instrument. temperature range 4℃~100℃, temperature uniformity ±0.4℃.
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