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Detection and identification of Diaporthe caulivora
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Basic data
| Standard ID | GB/T 45585-2025 (GB/T45585-2025) |
| Description (Translated English) | Detection and identification of Diaporthe caulivora |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B16 |
| Classification of International Standard | 65.020.20 |
| Word Count Estimation | 22,280 |
| Date of Issue | 2025-04-25 |
| Date of Implementation | 2025-11-01 |
| Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration |
GB/T 45585-2025: Detection and identification of Diaporthe caulivora (Athow and Caldwell) Santos, Vrandecic and Phillips and Diaporthe aspalathi E. Janse, Castl. and Crous
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 65.020.20
CCSB16
National Standard of the People's Republic of China
Quarantine and Identification Methods for Soybean Stem Canker Pathogen
Released on 2025-04-25
2025-11-01 Implementation
State Administration for Market Regulation
The National Standardization Administration issued
Foreword
This document was drafted in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents".
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document is proposed and coordinated by the National Technical Committee on Plant Quarantine Standardization (SAC/TC271).
This document was drafted by. China Institute of Inspection and Quarantine, Hangzhou Customs Technical Center, Huangpu Customs Technical Center, Nanjing Customs Animal and Plant and Food Inspection Center,
Simao Customs Comprehensive Technical Service Center, Ningbo Inspection and Quarantine Science and Technology Research Institute, and Wuhan Customs Technical Center.
The main drafters of this document are. Wu Pinshan, Lei Rong, Chen Wujian, Zhang Haipeng, Li Bin, Zhou Jian, Sun Xiwen, Yang Li, Duan Weijun, Huali, and Wang Zhenhua.
Quarantine and Identification Methods for Soybean Stem Canker Pathogen
1 Scope
This document describes the isolation, culture, morphological identification and molecular biological detection methods of soybean northern stem canker pathogen and soybean southern stem canker pathogen.
This document applies to the quarantine identification and monitoring of soybean stem canker pathogens in soybean seeds and soybean plants.
2 Normative references
The contents of the following documents constitute the essential clauses of this document through normative references in this text. Among them, the referenced documents with dates are.
Only the version corresponding to that date is applicable to this document; for undated referenced documents, the latest version (including all amendments) is applicable to this document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Recombinase polymerase amplification
A nucleic acid amplification technology in which multiple enzymes such as recombinase, single-stranded DNA binding protein (SSB) and strand-displacing DNA polymerase work together at a constant temperature between 37℃ and 42℃ to amplify DNA at a constant temperature.
4 Basic information about pathogens
Bacteria), Diaportheaspalathi E.Jansen, Castl.
English name. soybean stem canker.
Classification. Fungi, Ascomycota, Sordariomycetes, Di-
aporthales), Diaporthaceae, Diaporthe Nits., asexual stage is Phomopsis
(Phomopsis spp.).
5 Principles of the method
Based on the disease symptoms, morphological characteristics and molecular biological characteristics of the isolated bacteria, conventional PCR, real-time fluorescence PCR and
The principle of recombinase polymerase amplification (RPA) of specific fragments is used to conduct comprehensive quarantine identification of pathogens.
...
