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Diagnostic techniques for sheep pox and goat pox
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Basic data
| Standard ID | GB/T 45105-2024 (GB/T45105-2024) |
| Description (Translated English) | Diagnostic techniques for sheep pox and goat pox |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B41 |
| Classification of International Standard | 11.220 |
| Word Count Estimation | 30,353 |
| Date of Issue | 2024-12-31 |
| Date of Implementation | 2025-07-01 |
| Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration |
GB/T 45105-2024: Diagnostic techniques for sheep pox and goat pox
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
National Standard of the People's Republic of China
ICS 11.220CCS B 41
Sheeppox and goatpox diagnostic techniques
2024-12-31 Release
2025-07-01 Implementation
State Administration for Market Regulation
The National Standardization Administration issued
Table of Contents
Preface Ⅲ
Introduction Ⅳ
1 Scope...1
2 Normative references ...1
3 Terms and Definitions 1
4 Abbreviations 1
5 Clinical diagnosis 1
6 Sample collection, storage and transportation ...2
7 Virus isolation and identification 3
8 Histological examination...4
9 PCR test...4
10 Real-time fluorescence PCR detection...6
11 LAMP assay...7
12 Virus Neutralization Test 8
13 ELISA...9
14 Comprehensive judgment...10
Appendix A (Normative) Preparation of Commonly Used Reagent Solutions 11
Appendix B (Normative) Preparation of PCR Solution 12
Appendix C (Informative) Comparison of CaPV PCR Test Results 15
Appendix D (Informative) Comparison of CaPV Real-time Fluorescence PCR Detection Results ...16
Appendix E (Informative) CaPV LAMP test results refer to Figure 17
Appendix F (Informative) Calculation of neutralizing antibody titer by Reed-Muench method 18
Appendix G (Informative) Preparation of CaPV recombinant P32 protein antigen 19
Appendix H (Informative) Preparation of Negative and Positive Controls 20
Appendix I (Normative) Preparation of ELISA reagents 21
References ...22
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting is required.
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China.
This document is under the jurisdiction of the National Technical Committee on Animal Health Standardization (SAC/TC 181).
This document was drafted by. China Animal Disease Prevention and Control Center, Qinghai Animal Disease Prevention and Control Center, Dalian Minzu University, Qinghai
Hai Province Yak Breeding and Extension Service Center, China Inspection and Quarantine Scientific Research Institute, Hebei Agricultural University, Xinjiang Uygur Autonomous Region Animal Disease Prevention and Control Center
Animal Disease Prevention and Control Center, Xinjiang Production and Construction Corps Animal Disease Prevention and Control Center, Tibet Autonomous Region Animal Disease Prevention and Control Center, Sichuan Provincial Animal Disease Prevention and Control Center
Animal Disease Prevention and Control Center, Heilongjiang Provincial Animal Disease Prevention and Control Center, Jiangxi Provincial Animal Disease Prevention and Control Center, Hainan Provincial Animal Disease Prevention and Control Center
Disease Prevention and Control Center, Beijing Yisenbao Biotechnology Co., Ltd., Shandong Binzhou Animal Husbandry and Veterinary Research Institute, Xining Animal Disease Prevention
Control Center, Chongqing Animal Disease Prevention and Control Center, Shandong Animal Disease Prevention and Control Center, Zibo Boshan District Animal Husbandry and Fishery Service Center
Beijing Animal Disease Prevention and Control Center, Shaanxi Provincial Animal Disease Prevention and Control Center, Hami Animal Disease Prevention and Control Center
Center, Urumqi Animal Disease Control and Diagnosis Center, Yili Prefecture Animal Disease Control and Diagnosis Center, Guangxi Zhuang Autonomous Region Animal Disease Control and Diagnosis Center
Prevention and Control Center, Chengdu Customs Technical Center, Shanxi Provincial Animal Disease Prevention and Control Center, and Hebei Provincial Animal Disease Prevention and Control Center.
The main drafters of this document are. Sun Yu, Song Xiaohui, Cao Jijuan, Li Qi, Zhai Xinyan, Xiao Ying, Gu Xiaoxue, Wang Chuanbin, Yang Lin, Cai Jinshan,
Jing Jianwu, Kan Wei, Yuan Wanzhe, Yang Aiguo, Li Shengqiong, Gao Lu, Su Guicheng, Hu Guangwei, Zheng Sisi, Meng Ru, Wang Shuqin, Gan Ping, Feng Chunyan,
Zhao Jianbo, Zhong Yingmei, Zhang Xu, Yang Tao, Deji Yuzhen, Gesang Yangzong, Zhong Xinting, Jumabek Xialabai, Ma Xiaoyan, Yang Yiping,
Li Qingxia, Chi Qingan, Chen Ronggui, Wang Tao, Sun Hang, Liu Yingwei, Xu Qi, Bi Yiming, Wang Xinjie, Sun Xiaoming, Gao Shanshan, Bai Xuedong, Xu Peng,
Wang Jinliang, Shen Zhiqiang, Zeng Zheng, Luo Lu, Lan Zou Ran, Zhang Yue, Zhao Haojun, Li Dongliang, Zhao Guangming, Shi Kaichuang, Mo Shenglan, Lin Hua, Lei Yuping,
Xu Yujing.
Introduction
Sheep pox and goat pox (hereafter referred to as sheep pox) are caused by sheep pox virus (SPV) and goat pox virus (SPV), respectively.
Goatpox virus (GPV) is the cause of the disease. Both sheeppox virus and goatpox virus belong to the Poxviridae family, vertebrate poxviruses
Subfamily (Chordopoxvirdae), Capripoxvirus (CaPV). my country classifies it as a Category II animal epidemic disease.
It is also a disease listed by the World Organization for Animal Health (WOAH). Infected animals show fever, widespread papules on the skin, mucous membranes, and organ surfaces.
Rash or nodules, skin edema, swollen lymph nodes, weight loss, a significant decrease in milk production, and in severe cases, death. Goat pox was first reported in BC
Sheeppox was first discovered in England in the 13th century, with an incidence of 50% to 80% and a mortality rate of 20% to 80%.
The mortality rate of sheep can reach 100%. There are reports of sheep pox outbreaks and epidemics in countries around the world. Currently, it is common in northern Africa, the Middle East, and parts of Asia.
Sheep pox is a major obstacle to the introduction of valuable sheep and goat breeds and large-scale sheep farming, and it poses a serious threat to
The clinical symptoms of sheep pox are easily confused with sheep pseudotuberculosis and sheep infectious pustule, and laboratory differential diagnosis is required.
This document refers to the relevant contents of the Handbook of Terrestrial Animal Diagnostic Tests and Vaccines (WOAH), and the technical methods are consistent with those recommended by WOAH.
The standard method is consistent.
Sheeppox and goatpox diagnostic techniques
1 Scope
This document describes the clinical diagnosis, virus isolation and identification, PCR detection, real-time PCR detection,
Diagnostic methods such as LAMP assay, virus neutralization test, ELISA, etc.
This document applies to the clinical diagnosis, laboratory testing, epidemiological investigation and inspection and quarantine of sheeppox.
2 Normative references
The contents of the following documents constitute the essential terms of this document through the regulatory references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to
This document.
GB 19489 General requirements for laboratory biosafety
3 Terms and definitions
There are no terms or definitions that require definition in this document.
4 Abbreviations
The following abbreviations apply to this document.
CaPV. capripoxvirus
CPE. cytopathic effect
Ct value. cycle threshold
DNA. deoxyribonucleic acid
EDTA. ethylene diamine tetraacetic acid
ELISA. enzyme linked immunosorbent assay
LAMP. loop-mediated isothermal amplification
MEM. minimum essential medium
OD. optical density
PBS. phosphate buffer saline
PCR. polymerase chain reaction
Taq. Thermus aquaticus
TCID50.50% tissue culture infective dose
5 Clinical diagnosis
5.1 Epidemiology
5.1.1 Susceptible animals
Sheep and goats of different breeds, genders and ages are susceptible, lambs are more susceptible than adult sheep, and other livestock are rarely infected.
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