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Diagnostic techniques for enzootic abortion of ewes
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Basic data
| Standard ID | GB/T 45100-2024 (GB/T45100-2024) |
| Description (Translated English) | Diagnostic techniques for enzootic abortion of ewes |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B41 |
| Classification of International Standard | 11.220 |
| Word Count Estimation | 22,286 |
| Date of Issue | 2024-12-31 |
| Date of Implementation | 2025-07-01 |
| Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration |
GB/T 45100-2024: Diagnostic techniques for enzootic abortion of ewes
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 11.220
CCSB41
National Standard of the People's Republic of China
Diagnostic Techniques for Endemic Abortion in Sheep
Released on 2024-12-31
2025-07-01 Implementation
State Administration for Market Regulation
The National Standardization Administration issued
Table of Contents
Preface III
Introduction IV
1 Scope 1
2 Normative references 1
3 Terms and Definitions 1
4 Abbreviations 1
5 Biosafety measures 1
6 Clinical Diagnosis 1
7 Sample collection and storage 2
8 Bacterial isolation and culture 3
9 PCR 4
10 Fluorescence PCR 5
11 ELISA 7
12 Comprehensive judgment 8
Appendix A (Normative) Sample collection and storage and preparation of bacterial isolation and culture reagents 9
Appendix B (Normative) Preparation of PCR Reagents 10
Appendix C (Informative) Preparation of Chlamydia abortus Pomp90 Recombinant Protein 11
Appendix D (Normative) Preparation method of enzyme-linked immunosorbent assay reagents 12
Appendix E (Informative) Preparation of Negative and Positive Control Serum 13
Reference 14
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting.
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China.
This document is under the jurisdiction of the National Technical Committee on Animal Health Standardization (SAC/TC181).
This document was drafted by. China Animal Health and Epidemiology Center, Inner Mongolia Autonomous Region Animal Disease Prevention and Control Center, Hulunbuir
Municipal Animal Disease Prevention and Control Center, Guangxi Zhuang Autonomous Region Animal Disease Prevention and Control Center, Shandong Binzhou Animal Husbandry and Veterinary Research Institute, Xinjiang
Uygur Autonomous Region Animal Health Supervision Institute, Qingdao Animal Disease Prevention and Control Center.
The main drafters of this document are. Sun Xiangxiang, Sun Shufang, Sun Mingjun, Fan Xiaoxu, Liu Mengda, Zhang Haobo, Yan Xin, Tian Lili, Sun Shixiong,
Shao Weixing, Wang Jianlong, Temurbagen, Guo Yu, Wang Wei, Wang Guanyu, Xiong Yi, He Qisong, Wang Jinliang, Yan Hao, and Li Yan.
Introduction
Ovinechlamydiosis is an infectious disease caused by Chlamydia abortus, characterized by fever, miscarriage, stillbirth and
The main characteristic is the birth of weak lambs. my country lists it as a Category III animal disease, and the World Organization for Animal Health (WOAH) lists it as a listed disease.
People are infected through contact with abortion products, birth products or in the laboratory, and develop subclinical symptoms or acute influenza-like illness.
Chlamydia abortus)" and related literature, PCR, fluorescent PCR and ELISA methods for endemic abortion in sheep were established.
Diagnostic Techniques for Endemic Abortion in Sheep
1 Scope
This document describes the clinical diagnosis of endemic abortion in sheep, sample collection and processing, polymerase chain reaction (PCR), fluorescent PCR and enzyme
ELISA is a diagnostic method.
This document applies to the diagnosis and monitoring of endemic abortion in sheep and goats.
2 Normative references
The contents of the following documents constitute essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to
This document.
GB 19489 General requirements for laboratory biosafety
3 Terms and definitions
There are no terms or definitions that require definition in this document.
4 Abbreviations
The following abbreviations apply to this document.
OD. Optical density
5 Biosafety measures
When conducting laboratory diagnosis of endemic abortion in sheep, such as sample processing, isolation and culture, nucleic acid extraction, etc., GB 19489 should be followed.
6 Clinical diagnosis
6.1 Epidemiology
6.1.1 Mainly infects sheep and goats. Sheep of different ages can be infected. Many domestic animals and humans can be infected.
6.1.2 The source of infection is sick sheep and healthy sheep carrying the bacteria. It is mainly transmitted through the digestive tract, respiratory tract and reproductive tract, and can also be transmitted through tears, milk, urine
It is transmitted through vaginal discharge, feces, nasal secretions, aborted placenta, fetus, amniotic fluid, etc.
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