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Diagnostic techniques for novel waterfowl astrovirus disease
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Basic data
| Standard ID | GB/T 45093-2024 (GB/T45093-2024) |
| Description (Translated English) | Diagnostic techniques for novel waterfowl astrovirus disease |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B41 |
| Classification of International Standard | 11.220 |
| Word Count Estimation | 26,230 |
| Date of Issue | 2024-12-31 |
| Date of Implementation | 2025-07-01 |
| Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration |
GB/T 45093-2024: Diagnostic techniques for novel waterfowl astrovirus disease
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 11.220
CCSB41
National Standard of the People's Republic of China
Diagnostic technology for new astrovirus disease in waterfowl
Released on 2024-12-31
2025-07-01 Implementation
State Administration for Market Regulation
The National Standardization Administration issued
Table of Contents
Preface III
Introduction IV
1 Scope 1
2 Normative references 1
3 Terms and Definitions 1
4 Abbreviations 1
5 Clinical diagnosis 1
6 Sample collection and processing 2
7 Virus Isolation 3
8 RT-PCR 4
9 Real-time fluorescence quantitative RT-PCR 6
10 Indirect ELISA 7
11 Comprehensive judgment 9
Appendix A (Informative) Clinical Cases Pathological Changes in Ocular Observation Figure 10
Appendix B (Informative) Solution Preparation (reagents must be analytically pure) 12
Appendix C (Informative) Changes in the body of infected embryos Figure 13
Appendix D (Informative) Primers and Probes 14
Appendix E (Informative) Positive Control Virus (SDPY) 15
Appendix F (Informative) Total RNA Extraction Procedure (TRIZOL Method) 16
Appendix G (Informative) Reference strain sequence 17
Appendix H (Informative) RT-PCR electrophoresis diagram 18
Appendix I (Informative) ELISA Antigen and Serum Preparation 19
Reference 20
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting.
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China.
This document is under the jurisdiction of the National Technical Committee on Animal Health Standardization (SAC/TC181).
This document was drafted by. Shandong Agricultural University, Anhui Qiangying Food Group Co., Ltd., Qingdao Yibang Bioengineering Co., Ltd., Dezhou University
Institute of Animal Health and Epidemiology, China Agricultural University, Qufu Normal University, Guizhou Animal Husbandry and Veterinary Research Institute, Shandong Zhongxin Food
Group Co., Ltd., Beijing Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences, Shandong Hekangyuan Biological Breeding Co., Ltd., Jiangsu Yike
Food Group Co., Ltd. and Shandong Rongda Agricultural Development Co., Ltd.
The main drafters of this document are. Tang Yi, Diao Youxiang, Hao Dongmin, Liu Dong, Jiang Xiaoning, Li Yang, Cui Yitong, Li Fang, Zhang Dabing, Chen Hao, Feng Yulong,
Cui Xuezhi, Guo Zhanbao, Wang Sheng, Wang Jianhua, Liu Haitao, Zhang Huiling, Wei Feng, Yu Xiaohui, Han Qinghai, Wang Wei, and Hu Jingdong.
Introduction
RNA viruses can induce symptoms such as visceral and joint gout in waterfowl, causing great harm to the safety of waterfowl germplasm resources.
The new waterfowl astrovirus disease was first reported in Shandong, Anhui, Jiangsu, Liaoning, Henan, Guangdong and other places in my country in early.2017.
An infectious disease characterized by gout that breaks out in a flock of geese or ducks. The disease may infect goslings and ducklings of different ages, causing
Serious urate deposition in the internal organs and joint cavities of poultry is highly contagious and has a high mortality rate, causing economic losses to my country's waterfowl farming industry.
huge.
The disease is initially diagnosed based on its characteristic clinical symptoms and visual lesions, and then confirmed through laboratory pathogen testing.
Since the disease was first reported, there is no effective treatment and no conventional vaccine to prevent it.
To provide standardized diagnostic technology for rapid and accurate diagnosis of new astrovirus diseases in waterfowl flocks, so as to detect infected flocks as early as possible and take quarantine measures.
Isolation measures can be taken to reduce the economic losses caused by new astrovirus infection in waterfowl.
Diagnostic technology for new astrovirus disease in waterfowl
1 Scope
This document describes the clinical diagnosis of a novel waterfowl astrovirus, sample collection and processing, virus isolation, RT-PCR, and real-time fluorescence quantification.
Laboratory detection methods such as RT-PCR and indirect ELISA.
This document applies to the diagnosis, surveillance and epidemiological investigation of novel astrovirus diseases in waterfowl.
2 Normative references
The contents of the following documents constitute the essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to
This document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
NY/T 541 Technical Specification for Collection, Storage and Transportation of Veterinary Diagnostic Samples
3 Terms and definitions
There are no terms or definitions that require definition in this document.
4 Abbreviations
The following abbreviations apply to this document.
Ct value. Cycle threshold
RNA. Ribonucleic acid
TAE. Tris-acetic acid-EDTA buffer
5 Clinical diagnosis
5.1 Epidemiological characteristics
5.1.1 The disease can occur throughout the year, but is more common in winter and spring.
5.1.2 All breeds of geese and ducks can be infected and fall ill.
5.1.3 The onset of disease in geese is seen within 3 weeks of age, with the highest incidence occurring between 5 and 20 days of age. The onset of disease in ducks is seen between 2 and 11 days of age.
5.1.4 The infection rate of geese is about 80%, and the mortality rate ranges from 2% to 60%; the infection rate of ducks is about 50%, and the mortality rate ranges from 5% to 20%.
Up to 30%.
...
