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Determination of Candida utilis in microbial feed additives
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Basic data
| Standard ID | GB/T 45033-2024 (GB/T45033-2024) |
| Description (Translated English) | Determination of Candida utilis in microbial feed additives |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B46 |
| Classification of International Standard | 65.120 |
| Word Count Estimation | 18,167 |
| Date of Issue | 2024-11-28 |
| Date of Implementation | 2025-06-01 |
| Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration |
GB/T 45033-2024: Determination of Candida utilis in microbial feed additives
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of Candida utilis in microbial feed additives
Determination of Candida utilis in feed microbial preparations
ICS 65.120
CCS B 46
National Standard of the People's Republic of China
Released on 2024-11-28
2025-06-01 Implementation
State Administration for Market Regulation
The National Standardization Administration issued
Table of Contents
Preface ... Ⅲ
1 Scope ... 1
2 Normative references ... 1
3 Terms and Definitions ... 1
4 Reagents or Materials ... 1
5 Instruments and equipment ... 1
6 Sampling ... 1
7 Test steps ... 2
8 Experimental data processing ... 3
Appendix A (Informative) Culture medium ... 5
Appendix B (Normative) Physiological and biochemical tests 6
Appendix C (Normative) Molecular Biological Identification 9
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting is required.
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document was proposed and coordinated by the National Feed Industry Standardization Technical Committee (SAC/TC 76).
This document was drafted by. Shandong Baolaililai Bioengineering Co., Ltd., Angel Yeast Co., Ltd., Beijing Dabeinong Science and Technology
Group Co., Ltd., Zhejiang Kefeng Biotechnology Co., Ltd., Tangshan Top Biotechnology Co., Ltd., Yihai Kerry (Fangchenggang) Biotechnology Co., Ltd.
OBJECT TECHNOLOGY CO., LTD.
The main drafters of this document are. Gu Wei, Shan Baolong, Xu Haiyan, Xie Quanxi, Xin Guoqin, Wang Xiangyan, Qi Xiuye, Tan Xianwu, Liu Xuelian,
Zhang Tingzhou, Zhou Xueling, and Ma Lizhou.
Determination of Candida utilis in feed microbial preparations
1 Scope
This document describes a method for the determination of the count of Candida utilis in microbial preparations for feed.
This document applies to the determination of feed microbial preparations containing Candida utilis.
2 Normative references
The contents of the following documents constitute the essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies.
in this document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
GB/T 42959 Sampling for feed microbiological testing
3 Terms and definitions
There are no terms or definitions that require definition in this document.
4 Reagents or materials
Unless otherwise specified, only analytical grade reagents were used.
4.1 Water. GB/T 6682, grade 3.
4.2 0.85% sterile saline. Accurately weigh 8.5 g of sodium chloride and add it to 1000 mL of water. Stir until completely dissolved. After packaging, place the mixture at 121 °C.
Sterilize for 30 min and set aside.
4.3 Red Bengal agar medium. see Appendix A, A.1.
4.4 Yeast peptone dextrose (YPD) agar medium. see A.2.
5.Instruments and equipment
In addition to routine sterilization and culture equipment in microbiology laboratories, other instruments and equipment are as follows.
5.1 Incubator. controllable accuracy ±1 ℃.
5.2 Balance. sensitivity is 0.1 g.
5.3 Optical microscope. 400x to 1000x.
5.4 Slap-type homogenizer and sterile homogenizing bag.
5.5 Vortex mixer.
5.6 Sterile culture dish. 90 mm in diameter.
5.7 Polymerase chain reaction (PCR) instrument.
6 Sampling
Implement in accordance with GB/T 42959.
...
