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Determination of β-apo-8′-carotenoic acid ethyl ester in feeds - High performance liquid chromatography
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Basic data
| Standard ID | GB/T 44965-2024 (GB/T44965-2024) |
| Description (Translated English) | Determination of ��-apo-8��-carotenoic acid ethyl ester in feeds - High performance liquid chromatography |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B46 |
| Classification of International Standard | 65.120 |
| Word Count Estimation | 10,160 |
| Date of Issue | 2024-11-28 |
| Date of Implementation | 2025-06-01 |
| Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration |
GB/T 44965-2024: Determination of β-apo-8′-carotenoic acid ethyl ester in feeds - High performance liquid chromatography
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Determination of β-apo-8′-carotenoic acid ethyl ester in feeds - High performance liquid chromatography
β﹘apo﹘8′﹘carotene acid ethyl ester in feed
HPLC
Determination of β﹘apo﹘8′﹘carotenoic acid ethyl ester in feeds-
High performance liquid chromatography
ICS 65.120
CCS B 46
National Standard of the People's Republic of China
Released on 2024-11-28
2025-06-01 Implementation
State Administration for Market Regulation
The National Standardization Administration issued
Table of Contents
Preface ... Ⅲ
1 Scope ... 1
2 Normative references ... 1
3 Terms and Definitions ... 1
4 Principle ... 1
5 Reagents or materials ... 1
6 Instruments and Equipment ... 2
7 samples ... 2
8 Test steps ... 2
8.1 Preparation of sample solution 2
8.2 Determination 3
9 Experimental data processing ... 3
10 Precision ... 4
Appendix A (Informative) β﹘Apo﹘8′﹘Carotene Acid Ethyl Ester HPLC Chromatography ... 5
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting is required.
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document was proposed and coordinated by the National Feed Industry Standardization Technical Committee (SAC/TC 76).
This document was drafted by. Guizhou Provincial Veterinary Drug and Feed Testing Institute, Zhaoqing Juyuan Biochemical Co., Ltd., Guangzhou Zhitech Biotechnology Co., Ltd.
company.
The main drafters of this document are. Zhao Gui, Zhang Lijie, Sun Bodong, Wang Qinghong, Su Meicheng, Sun Zhenzheng, Luan Qingxiang, Huang Xin, Zhu Jimei,
Deng Qingsheng, Liao Huan, Wang Xuan, Jin Xiaofeng, Xie Lili, Chen Bing, Xiao Feng, Guo Ping, Zhou Yilin, Yang Qiang.
β﹘apo﹘8′﹘carotene acid ethyl ester in feed
HPLC
1 Scope
This document describes a method for the determination of β-apo-8′-carotene acid ethyl ester in feeds by high performance liquid chromatography.
This document applies to β﹘apo﹘8′﹘carotene acid ethyl ester in compound feed, concentrated feed, concentrate supplement and additive premix feed.
Determination of.
The detection limit of β﹘apo﹘8′﹘carotene acid ethyl ester in compound feed, concentrated feed and concentrate supplement in this document is 0.15 mg/kg,
The limit of quantification is 0.50 mg/kg; the detection limit of β﹘apo﹘8′﹘carotene acid ethyl ester in additive premix feed is 1.5 mg/kg and the limit of quantification is
It is 5.0 mg/kg.
2 Normative references
The contents of the following documents constitute the essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies.
in this document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
GB/T 20195 Preparation of animal feed samples
3 Terms and definitions
There are no terms or definitions that require definition in this document.
4 Principle
The β-carotene acid ethyl ester in the sample was dispersed in water, extracted with ethanol and dichloromethane, and purified with a solid phase extraction column (prepared with additives).
Mixed feeds excluded), determined by high performance liquid chromatography and quantified by external standard method.
5 Reagents or materials
Unless otherwise specified, only analytical grade reagents were used.
5.1 Water. GB/T 6682, Grade 1.
5.2 Dichloromethane.
5.3 Anhydrous ethanol.
5.4 Acetonitrile. chromatographic grade.
5.5 Methanol. chromatographic grade.
5.6 2,6-butylene glycol (BHT).
5.7 50% acetonitrile solution. Take 100 mL of acetonitrile (5.4), dilute to.200 mL with water, and mix well.
5.8 Standard stock solution (200 μg/mL). Weigh β-apo-8′-carotene acid ethyl ester standard (CAS No.
1109﹘11﹘1, purity ≥98.5%), place an appropriate amount (accurate to 0.01 mg) in a brown volumetric flask, and dissolve the fixed
...
