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GB/T 44738-2024: Determination of feed pectinase activity
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GB/T 44738-2024: Determination of feed pectinase activity

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Determination of feed pectinase activity Determination of feed pectinase activity ICS 65.120 CCS B 46 National Standard of the People's Republic of China Released on October 26, 2024 Implementation on May 1, 2025 State Administration for Market Regulation The National Standardization Administration issued

Table of Contents

Preface... Ⅲ 1 Scope... 1 2 Normative references... 1 3 Terms and Definitions 1 4 Principles... 1 5 Reagents or materials 1 6 Instruments and Equipment... 2 7 samples... 2 8 Test steps... 2 8.1 Preparation of sample solution 2 8.2 Drawing a standard curve 3 8.3 Determination 3 9 Experimental data processing... 3 10 Precision... 4 Preface This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents" Drafting is required. Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents. This document is proposed and coordinated by the National Feed Industry Standardization Technical Committee (SAC/TC 76). This document was drafted by. Wuhan Xinhuayang Biotechnology Co., Ltd., Beijing Xindayang Technology Development Co., Ltd., Guangdong Yidoli Biotechnology Co., Ltd. Technology Co., Ltd., Shandong Longkote Enzyme Preparation Co., Ltd., and Novozymes (China) Investment Co., Ltd. The main drafters of this document are. Zhou Ying, Xu Li, Zhan Zhichun, Zhao Lifang, Feng Guohua, Zhang Faling, Sun Xuewei, Sudan, Yu Yan, Li Yangyuan, Wang Kefen and Wang Jinfeng. Determination of feed pectinase activity

1 Scope

This document describes a method for the spectrophotometric determination of the activity of feed pectinase. This document is applicable to the determination of pectinase activity in feed additive pectinase and mixed feed additive enzyme preparations containing pectinase. This document is not applicable to the determination of alkaline pectinase activity. The limit of quantification of this method is 10 U/g (or U/mL).

2 Normative references

The contents of the following documents constitute the essential clauses of this document through normative references in this document. For referenced documents without a date, only the latest version (including all amendments) applies to this document. In this document. GB/T 6682 Specifications and test methods for water used in analytical laboratories GB/T 20195 Preparation of animal feed samples 3 Terms and Definitions The following terms and definitions apply to this document. 3.1 Pectinase activity unit At 37 °C and pH 3.5, the amount of reducing sugar released from a pectin solution with a mass concentration of 2.25 mg/mL per hour is The amount of enzyme required. Note. Indicated by U.

4 Principles

Pectinase hydrolyzes pectin solution to produce reducing sugar, which is then developed with 3,5-dinitrosalicylic acid and measured with a spectrophotometer to calculate the pectin content. Enzyme activity. 5 Reagents or materials Unless otherwise stated, only analytical grade reagents were used. 5.1 Water. GB/T 6682, Grade III. 5.2 Sodium hydroxide solution (200 g/L). Weigh 20.0 g of sodium hydroxide, dissolve it in water, cool to room temperature, and then dilute to 100 mL. 5.3 Citric acid solution (0.1 mol/L). Weigh 21.01 g of citric acid monohydrate, dissolve it in water and make up to 1000 mL. 5.4 Trisodium citrate solution (0.1 mol/L). Weigh 29.41 g of trisodium citrate dihydrate, dissolve it in water and make up to 1000 mL. 5.5 Citric acid﹘sodium citrate buffer (0.1 mol/L, pH=3.5). Take 700 mL of citric acid solution (5.3), trisodium citrate solution Add 300 mL of solution (5.4), mix well, and adjust the pH to 3.5 with citric acid solution (5.3) or trisodium citrate solution (5.4). 5.6 Pectin solution (3.0 mg/mL). Weigh 0.3 g of pectin (≥74% polygalacturonic acid, CAS No.. 9000﹘69﹘5) and ...