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Determination of gentamycin in feeds - Liquid chromatography-tandem mass spectrometry
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Basic data
| Standard ID | GB/T 44617-2024 (GB/T44617-2024) |
| Description (Translated English) | Determination of gentamycin in feeds - Liquid chromatography-tandem mass spectrometry |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B46 |
| Classification of International Standard | 65.120 |
| Word Count Estimation | 14,170 |
| Date of Issue | 2024-09-29 |
| Date of Implementation | 2025-04-01 |
| Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration |
GB/T 44617-2024: Determination of gentamycin in feeds - Liquid chromatography-tandem mass spectrometry
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Determination of gentamycin in feeds - Liquid chromatography-tandem mass spectrometry
Determination of Gentamycin in Feed
Liquid chromatography-tandem mass spectrometry
Determination of gentamycin in feeds-
Liquid chromatography-tandem mass spectrometry
ICS 65.120
CCS B 46
National Standard of the People's Republic of China
Released on 2024-09-29
2025-04-01 Implementation
State Administration for Market Regulation
The National Standardization Administration issued
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting is required.
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document was proposed and coordinated by the National Feed Industry Standardization Technical Committee (SAC/TC 76).
This document was drafted by. Institute of Agricultural Quality Standards and Testing Technology, Chinese Academy of Agricultural Sciences, Sichuan Weier Testing Technology Co., Ltd.
Co., Ltd., Beijing Technology and Business University, and Tongwei Agricultural Development Co., Ltd.
The main drafters of this document are. Suo Decheng, Zheng Lingyan, Zhang Fengping, Fan Xia, Chen Gang, An Zhixin, Zhang Lu, Feng Yuchao, Xiao Zhiming,
Wang Shi, Wu Jinyu, Dong Peng, Zhang Hangjun, Yao Ting, and Tang Jian.
Determination of Gentamycin in Feed
Liquid chromatography-tandem mass spectrometry
1 Scope
This document describes the principle, reagents or materials, equipment, sample preparation and analysis of gentamicin in feed by liquid chromatography-tandem mass spectrometry.
Products, test steps, and test data processing.
This document applies to gentamicin (in the form of gentamicin C1,
Determination of gentamicin C1a, gentamicin C2 and gentamicin C2a).
The detection limit of gentamicin in this document is 0.030 mg/kg, and the quantification limit is 0.100 mg/kg.
2 Normative references
The contents of the following documents constitute the essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies.
in this document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
GB/T 20195 Preparation of animal feed samples
3 Terms and definitions
There are no terms or definitions that require definition in this document.
4 Principle
Gentamycin in the sample was extracted with 5% trichloroacetic acid solution, purified by mixed cation exchange solid phase extraction column, and analyzed by liquid chromatography-tandem mass spectrometry.
The samples were determined by mass spectrometry, calibrated by matrix-matched standard curve, and quantified by external standard method.
5 Reagents or materials
Unless otherwise specified, only analytical grade reagents were used.
5.1 Water. GB/T 6682, Grade 1.
5.2 Ammonium formate. chromatographic grade.
5.3 Formic acid. chromatographic grade.
5.4 Methanol. chromatographic grade.
5.5 Acetonitrile. chromatographic grade.
5.6 0.1% formic acid solution. Take 1.0 mL of formic acid (5.3), dilute to 1 000 mL with water, and mix well.
5.7 0.1% formic acid acetonitrile solution. Take 1.0 mL of formic acid (5.3), dilute to 1 000 mL with acetonitrile, and mix well.
5.8 10% ammonia methanol solution. Take 10 mL of ammonia water, dilute it to 100 mL with methanol (5.4), and mix well.
5.9 1% heptafluorobutyric anhydride formic acid solution. Take 0.5 mL of heptafluorobutyric anhydride and dilute it to 50 mL with 0.1% formic acid solution (5.6) and mix well.
Prepare immediately before use.
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