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Diagnostic techniques for bovine and caprine parainfluenza virus type 3
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Basic data
| Standard ID | GB/T 44613-2024 (GB/T44613-2024) |
| Description (Translated English) | Diagnostic techniques for bovine and caprine parainfluenza virus type 3 |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B41 |
| Classification of International Standard | 11.220 |
| Word Count Estimation | 26,283 |
| Date of Issue | 2024-09-29 |
| Date of Implementation | 2025-04-01 |
| Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration |
GB/T 44613-2024: Diagnostic techniques for bovine and caprine parainfluenza virus type 3
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Diagnostic techniques for bovine and caprine parainfluenza virus type 3
Diagnostic technology of parainfluenza virus type 3 in cattle and sheep
ICS 11.220
CCS B 41
National Standard of the People's Republic of China
Released on 2024-09-29
2025-04-01 Implementation
State Administration for Market Regulation
The National Standardization Administration issued
Table of Contents
Preface ... Ⅲ
Introduction Ⅳ
1 Scope ... 1
2 Normative references ... 1
3 Terms and Definitions ... 1
4 Abbreviations ... 1
5 Clinical diagnosis ... 2
6 Sample collection, processing and storage ... 2
7 Virus isolation and identification ... 4
8 RT﹘PCR test ... 5
9 Real-time RT-PCR test ... 6
10 cELISA test ... 7
11 Virus Neutralization Test ... 9
12 Comprehensive judgment ... 11
Appendix A (Normative) Reagent Preparation ... 12
Appendix B (Normative) Primers and fluorescent probe sequences for viral nucleic acid detection ... 13
Appendix C (Normative) Preparation, primers and sequences of positive controls for RT﹘PCR and Real﹘time RT﹘PCR ... 14
Appendix D (Normative) RT﹘PCR and Real﹘time RT﹘PCR Reaction System and Reaction Procedure ... 16
Appendix E (Informative) RT﹘PCR and Real﹘time RT﹘PCR test results ... 18
Appendix F (Informative) Preparation of virus standard positive serum, standard negative serum and antigen-coated ELISA plate ... 20
Appendix G (Informative) Preparation of enzyme-labeled monoclonal antibodies 21
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting is required.
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China.
This document is under the jurisdiction of the National Technical Committee on Animal Health Standardization (SAC/TC 181).
This document was drafted by. Jilin Agricultural Science and Technology College, Jiangsu Academy of Agricultural Sciences, Jilin Provincial Animal Disease Prevention and Control Center, Jilin University
Institute of Animal Health and Epidemiology, China.
The main drafters of this document are. Guo Li, Sun Liang, Li Wenliang, Zhang Jinyu, Song Jiande, Liu Maojun, Yang Leilei, Mao Li, Li Jimong,
Cheng Yuening, Hao Fei, Sun Min, Wang Nan, Wang Zhenjun, Sun Na, Feng Xiaoyu.
Introduction
Bovine parainfluenza virus type 3 (BPIV3) and caprine parainfluenza virus type 3 (Caprine
parainfluenza virus type 3 (CPIV3) belongs to the Paramyxoviridae subfamily of the Paramyxoviridae family.
ovirinae) respiratory virus genus (Respirovirus), the nucleotide homology between the two is close to 81%, and there is a cross reaction in serology.
In.2001, the International Committee on Taxonomy of Viruses (ICTV) classified BPIV3 and
CPIV3 were renamed as Bovine Respirovirus 3 and Caprine Respirovirus 3.
Respirovirus 3), considering the continuity of relevant standards in my country, and for the convenience of users to query and apply, this document still uses the original virus naming
name.
BPIV3 mainly infects cattle, but may also infect deer, pigs, sheep, etc.; CPIV3 mainly infects goats and sheep.
The main clinical symptoms are fever, cough, and runny nose, and laboratory diagnosis is required for confirmation.
Clinical diagnosis of infection, pathogen detection (virus isolation and identification, RT﹘PCR, Real﹘time RT﹘PCR), serological detection (cEL﹘
ISA and virus neutralization test). When conducting pathogen detection on cattle samples, it is recommended to use RT﹘PCR or Real﹘time
RT﹘PCR method is recommended for detecting BPIV3; when conducting etiological testing on sheep samples, RT﹘PCR or Real﹘time RT﹘PCR is recommended.
The method can detect both CPIV3 and BPIV3.The two viruses have cross-reactivity in serology, so the serological method in this document can detect both
Detect antibodies to CPIV3 and BPIV3 infection.
Diagnostic technology of parainfluenza virus type 3 in cattle and sheep
1 Scope
This document describes the clinical diagnosis, virus isolation and characterization,
Diagnosis by RT﹘PCR, real-time fluorescence RT﹘PCR, competitive ELISA and virus neutralization assay
method.
This document applies to the diagnosis and detection of bovine parainfluenza virus type 3 infection and goat parainfluenza virus type 3 infection.
2 Normative references
The contents of the following documents constitute the essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies.
in this document.
NY/T 541 Technical Specification for Collection, Storage and Transportation of Veterinary Diagnostic Samples
3 Terms and definitions
There are no terms or definitions that require definition in this document.
4 Abbreviations
The following abbreviations apply to this document.
BPIV3.Bovine parainfluenza virus type 3
cELISA. Competitive enzyme﹘linked immunosorbent assay
CPE. Cytopathic effect
CPIV3.Caprine parainfluenza virus type 3
Ct value. Cycle threshold
DEPC. Diethyl oxydiformate
ELISA. Enzyme-linked immunosorbent assay
FBS. Fetal bovine serum
FITC. Fluorescein isothiocyanate
HAT. Selection medium (Hypoxanthine, Aminopterin, Thymidine)
HRP. Horseradish peroxidase
MDBK cells. Madin darby bovine kidney cells
MEM. Minimum essential medium
MOI. Multiplicity of infection
OD value. optical density
PBS. Phosphate buffered saline
PBST. Phosphate buffered saline with tween 20
PIV3.Parainfluenza virus type 3
...
