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Determination of methyl syringate in rape honey - High performance liquid chromatography method
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Basic data
| Standard ID | GB/T 44350-2024 (GB/T44350-2024) |
| Description (Translated English) | Determination of methyl syringate in rape honey - High performance liquid chromatography method |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B47 |
| Classification of International Standard | 67.180.10 |
| Word Count Estimation | 10,186 |
| Date of Issue | 2024-09-29 |
| Date of Implementation | 2025-04-01 |
| Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration |
GB/T 44350-2024: Determination of methyl syringate in rape honey - High performance liquid chromatography method
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Determination of methyl syringate in rape honey - High performance liquid chromatography method
Determination of Methyl Syringate in Rape Honey
High performance liquid chromatography
Determination of methyl syringate in rape honey-High performance liquid
chromatography method
ICS 67.180.10
CCS B 47
National Standard of the People's Republic of China
Released on 2024-09-29
2025-04-01 Implementation
State Administration for Market Regulation
The National Standardization Administration issued
Table of Contents
Preface ... Ⅲ
1 Scope ... 1
2 Normative references ... 1
3 Terms and Definitions ... 1
4 Principle ... 1
5 Reagents and Materials ... 1
6 Instruments and Equipment ... 2
7 Analysis steps ... 2
7.1 Sample preparation 2
7.2 Blank test ... 2
7.3 Parallel tests 2
7.4 Determination 2
8 Result calculation ... 3
9 Limit of Quantitation ... 3
10 Precision ... 3
Appendix A (Informative) HPLC Chromatogram of Methyl Syringate Standard Working Solution ... 4
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting is required.
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document was proposed by the China Federation of Supply and Marketing Cooperatives.
This document is under the jurisdiction of the National Technical Committee on Standardization of Bee Products (SAC/TC 601).
This document was drafted by. China University of Metrology, Anhui Mizhiyuan Food Group Co., Ltd., Guangzhou Jinji Technology Testing Co., Ltd.,
Kebiao Standard (Ningde) Technology Co., Ltd., Shenzhen Weibiao Testing Technology Co., Ltd., Nanjing Customs Animal and Plant and Food Testing Center, Jinhua
Municipal Academy of Agricultural Sciences, Bee Research Institute of Chinese Academy of Agricultural Sciences, Zhejiang Rural Revitalization Promotion Center, Greentown Agricultural Science Testing Technology Co., Ltd.
Zhejiang Huacai Testing Technology Co., Ltd., Zhejiang Provincial Animal Husbandry Technology Promotion and Breeding Livestock and Poultry Monitoring Center, Jiangsu Guoce Testing Technology Co., Ltd.
Co., Ltd., Shenzhen Yupeng Technology Co., Ltd., Qiqihar Inspection and Testing Center, Qingdao Zhongyi Monitoring Co., Ltd., and Huzhou Bee Industry Research Institute.
The main drafters of this document are. Li Hongliang, Zhu Xinli, Lin Hong, Su Xiaoling, Wu Fan, Qiao Jiangtao, Huang Jinjing, Wang Zhijun, Lin Chai,
He Li, Li Jiafen, Wang Layun, Li Kui, Lin Ying, Huang Yongming, Wang Weigang, Lou Xishan, Zhong Qiaoxia, Qiu Kai.
Determination of Methyl Syringate in Rape Honey
High performance liquid chromatography
1 Scope
This document describes a HPLC method for the determination of methyl syringate in rapeseed honey.
This document is applicable to the determination of methyl syringate content in rapeseed honey.
2 Normative references
The contents of the following documents constitute the essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies.
in this document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
3 Terms and definitions
There are no terms or definitions that require definition in this document.
4 Principle
Methyl syringate in rape honey samples was purified and enriched by weak anion exchange solid phase extraction column and then separated by C18 high performance liquid chromatography column.
The product was detected by UV or diode array detector (maximum absorption wavelength was 276 nm) and quantified by external standard peak area method.
5 Reagents and Materials
Unless otherwise specified, all reagents were of analytical grade and water was grade 1 water as specified in GB/T 6682.
5.1 Acetonitrile (chromatographic grade).
5.2 Ammonia water. concentration is 25% to 28% (mass fraction).
5.3 Methyl syringate (C10H12O5) standard. CAS No. 884﹘35﹘5, purity not less than 98%, or use standard substance/standard sample.
5.4 Methyl syringate standard stock solution (1 000 mg/L). Accurately weigh 0.100 0 g ± 0.000 1 g of methyl syringate standard (5.3) and
Dissolve the solution in acetonitrile (5.1) in a 25 mL beaker, transfer to a 100 mL volumetric flask, dilute to the mark, and shake well to obtain 1 000 mg/L
Methyl syringate standard stock solution. Store at 4 ℃ away from light, valid for 6 months.
5.5 Methyl syringate standard intermediate solution (100 mg/L). Accurately pipette 1.0 mL of 1 000 mg/L methyl syringate standard stock solution (5.4)
In a 10 mL volumetric flask, dilute with water and make up to volume, shake well to obtain a 100 mg/L methyl syringate standard intermediate solution. Place at 4 °C
Keep away from light, valid for 3 months.
5.6 Methyl syringate standard working solution. Accurately pipette 0.1 mL, 0.2 mL, 0.4 mL, 1.0 mL, 4.0 mL, and 10 mL of syringate, respectively.
The acid methyl ester standard intermediate solution (5.5) was added to a 10 mL volumetric flask, diluted with water and fixed to volume, and shaken to obtain a series of mass concentrations.
1 mg/L, 2 mg/L, 4 mg/L, 10 mg/L, 20 mg/L, 40 mg/L, ready for use.
The methyl syringate standard intermediate solution (5.5) together constitutes the methyl syringate standard working solution, which is used to prepare the standard curve (7.4.2).
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