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Determination of flavonol glycosides in epimedium extract as feed additive - High performance liquid chromatography
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Basic data
| Standard ID | GB/T 43810-2024 (GB/T43810-2024) |
| Description (Translated English) | Determination of flavonol glycosides in epimedium extract as feed additive - High performance liquid chromatography |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B46 |
| Classification of International Standard | 65.120 |
| Word Count Estimation | 10,184 |
| Date of Issue | 2024-03-15 |
| Date of Implementation | 2024-10-01 |
| Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration |
GB/T 43810-2024: Determination of flavonol glycosides in epimedium extract as feed additive - High performance liquid chromatography
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 65.120
CCSB46
National Standards of People's Republic of China
Feed Additive Epimedium Extract
Determination of flavonol glycosides by HPLC
Released on 2024-03-15
2024-10-01 Implementation
State Administration for Market Regulation
The National Standardization Administration issued
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting.
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document was proposed and coordinated by the National Feed Industry Standardization Technical Committee (SAC/TC76).
This document was drafted by. Beijing Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences, Institute of Medicinal Plants, Chinese Academy of Medical Sciences, Tianjin Institute of
Feder Technology Co., Ltd., Hunan Agricultural University, Beijing Ailu Biotechnology Co., Ltd., and Feed Research Institute of Chinese Academy of Agricultural Sciences.
The main drafters of this document are. Zhang Junmin, Zhang Huiyan, Zhao Qingyu, Tang Chaohua, Qin Yuchang, Guo Baolin, Deng Xuejuan, Zeng Jianguo, Yao Langqun,
Zhang, Jingjing, Liu, Xiubin, Guo, Xiaoqing, and Gu, Xu.
Feed Additive Epimedium Extract
Determination of flavonol glycosides by HPLC
1 Scope
This document describes a HPLC method for the determination of flavonol glycosides in the feed additive Epimedium brevicornum extract.
This document applies to the five flavonol glycosides (icariin, epimedin A, epimedin B, epimedin C) in the feed additive epimedin extract.
and Baohuoside I).
In this document, the limits of quantification for icariin, epimedin A, epimedin B, epimedin C and baucariside I are all 0.2 g/kg.
2 Normative references
The contents of the following documents constitute the essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to
This document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
GB/T 20195 Preparation of animal feed samples
3 Terms and definitions
There are no terms or definitions that require definition in this document.
4 Principle
The flavonol glycosides in the samples were extracted with methanol solution and determined by high performance liquid chromatography using the external standard method.
5 Reagents or materials
Unless otherwise specified, only analytical grade reagents were used.
5.1 Water. GB/T 6682, Grade 1.
5.2 Methanol. chromatographic grade.
5.3 Acetonitrile. chromatographic grade.
5.4 70% methanol solution. Measure 700 mL of methanol (5.2) into a 1000 mL volumetric flask, add water to make up to volume, and mix well.
5.5 Standard stock solution (2 mg/mL). Accurately weigh icariin (CAS. 489-32-7, purity ≥ 96%), epimedin A (CAS.
110623-72-8, purity ≥95%), epimedin B (CAS. 110623-73-9, purity ≥95%), epimedin C (CAS. 110642-44-9, purity
20 mg each (accurate to 0.01 mg) of baohuoside I (CAS. 113558-15-9, purity ≥ 95%) were placed in 10 mL brown
Dissolve in methanol (5.2) in a volumetric flask and make up to volume, mix well. Store below -18℃, valid for 3 months.
5.6 Mixed standard intermediate solution (200 mg/L). Accurately pipette 1 mL of each standard stock solution (5.5) into a 10 mL brown volumetric flask.
Add 70% methanol solution (5.4) to the volume and mix well. Store at 2℃~10℃, valid for 3 months.
5.7 Mixed standard series solution. Accurately pipette an appropriate amount of mixed standard intermediate solution (5.6) and prepare it with 70% methanol solution (5.4) to a mass concentration of
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