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Diagnostic techniques for schmallenberg disease
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Basic data
| Standard ID | GB/T 43159-2023 (GB/T43159-2023) |
| Description (Translated English) | Diagnostic techniques for schmallenberg disease |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B41 |
| Classification of International Standard | 11.220 |
| Word Count Estimation | 18,121 |
| Date of Issue | 2023-09-07 |
| Date of Implementation | 2024-04-01 |
| Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration |
GB/T 43159-2023: Diagnostic techniques for schmallenberg disease
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 11.220
CCSB41
National Standards of People's Republic of China
Schmallenberg disease diagnostic technology
Published on 2023-09-07
2024-04-01 Implementation
State Administration for Market Regulation
Released by the National Standardization Administration Committee
Table of contents
PrefaceⅠ
Introduction II
1 Scope 1
2 Normative reference documents 1
3 Terms and Definitions 1
4 Abbreviations 1
5 Biosecurity measures 2
6 Clinical Diagnosis 2
7 Sample collection and processing 2
8 Laboratory Diagnosis 3
9 Comprehensive judgment 8
Appendix A (Normative) Preparation of solutions related to virus isolation and serum neutralization test 9
Appendix B (informative) Fluorescence RT-PCR primer and probe sequence information10
Appendix C (normative) Preparation of DEPC treated water 11
Appendix D (Normative) Preparation of solutions related to blocking ELISA antibody detection 12
Appendix E (informative) Serum neutralization test (plaque reduction neutralization test) measurement example 13
Reference 14
Foreword
This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1.Structure and Drafting Rules of Standardization Documents"
Drafting.
Please note that some content in this document may be subject to patents. The publisher of this document assumes no responsibility for identifying patents.
This document is proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China.
This document is under the jurisdiction of the National Animal Health Standardization Technical Committee (SAC/TC181).
This document was drafted by. China Center for Animal Health and Epidemiology, Chinese Academy of Inspection and Quarantine.
The main drafters of this document. Xu Tiangang, Ge Shengqiang, Wu Shaoqiang, Zuo Yuanyuan, Zhang Yongqiang, Lu Yan, Chen Dongjie, Wang Shujuan, Su Hong, Qu Hailong,
Li Jinming, Bao Jingyue, Lin Xiangmei, Wang Zhiliang.
Introduction
A non-contact viral disease of ruminants (cattle, sheep, goats, etc.), mainly characterized by transient clinical symptoms (fever,
Diarrhea, reduced milk production, etc.), and congenital malformations in newborn animals. Schmallenberg virus belongs to the family Bunyaviridae
The virus is mainly transmitted through vector insects such as Culicoides, and can also be transmitted vertically through intrauterine infection. There is no evidence that the virus can infect humans.
The disease was first detected and named in the western German town of Schmallenberg in November.2011, and subsequently caused epidemics in most parts of Europe.
OK. The implementation of this document will improve the diagnosis and monitoring level of Schmallenberg disease in my country, take timely prevention and control measures, protect the health and safety of livestock and
Industrial development plays an important role.
This document was developed with reference to the World Organization for Animal Health's "Standard Manual of Diagnostic Tests and Vaccines for Terrestrial Animals" (2022 English version), and
It combines the new results of relevant technological research in my country.
Schmallenberg disease diagnostic technology
1 Scope
Isolation, fluorescence RT-PCR method, blocking ELISA antibody detection method, serum neutralization test and other laboratory diagnostic methods.
This document is applicable to the diagnosis, detection, quarantine, surveillance and epidemiology of Schmallenberg disease in cattle, sheep, goats and other ruminants.
investigation.
2 Normative reference documents
The contents of the following documents constitute essential provisions of this document through normative references in the text. Among them, the dated quotations
For undated referenced documents, only the version corresponding to that date applies to this document; for undated referenced documents, the latest version (including all amendments) applies to
this document.
GB 19489 General requirements for laboratory biosafety
3 Terms and definitions
There are no terms or definitions to be defined in this document.
4 Abbreviations
The following abbreviations apply to this document.
CPE. cytopathic effect
Ct. cycle valve domain (cyclethreshold)
FAM. 6-carboxy-fluorescein
OD. optical density
RNA. ribonucleic acid
SBV. Schmalenberg virus
...
