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Leather and fur - Determination of degradability by micro-organisms
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Basic data
| Standard ID | GB/T 43006-2023 (GB/T43006-2023) |
| Description (Translated English) | Leather and fur - Determination of degradability by micro-organisms |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | Y46 |
| Classification of International Standard | 59.140.30 |
| Word Count Estimation | 25,212 |
| Date of Issue | 2023-09-07 |
| Date of Implementation | 2024-04-01 |
| Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration |
GB/T 43006-2023: Leather and fur - Determination of degradability by micro-organisms
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 59.140.30
CCSY46
National Standards of People's Republic of China
Determination of microbial degradability of leather and fur
Published on 2023-09-07
2024-04-01 Implementation
State Administration for Market Regulation
Released by the National Standardization Administration Committee
Table of contents
Preface III
1 Scope 1
2 Normative reference documents 1
3 Terms and Definitions 1
4 Symbols and abbreviations1
5 Principle 1
5.1 General 1
5.2 Method A. Titration method to evaluate microbial degradability 2
5.3 Method B. IR method to evaluate microbial degradability 2
6 Reagents and Materials 2
7 Instruments and equipment2
8 Test step 5
8.1 Collection and preparation of inoculants5
8.2 Preparation of test samples and reference materials5
8.3 Test conditions and culture time 5
8.4 Test end judgment 5
9 Quantitative Analysis 6
9.1 Titration method to evaluate microbial degradability (Method A) 6
9.2 IR method to evaluate microbial degradability (Method B) 7
10 The result means 10
11 Reliability of results10
12 Test Report 10
Appendix A (informative) Structural changes of this document compared with ISO.20136.202011
Appendix B (informative) Technical differences between this document and ISO.20136.2020 and their reasons 13
Appendix C (informative) Determination of biodegradation rate and degradation rate 14
Appendix D (informative) Quantitative determination of microbial degradability of leather16
Appendix E (informative) Comparison of the microbial degradability of type I collagen by inoculants from different sources20
Foreword
This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1.Structure and Drafting Rules of Standardization Documents"
Drafting.
This document is modified to adopt ISO.20136.2020 "Determination of microbial degradability of leather".
Compared with ISO.20136.2020, this document has many adjustments in structure. A comparison list of structural number changes between the two documents
See Appendix A.
Compared with ISO.20136.2020, there are many technical differences between this document and the clauses involved in the outer margin of the page.
Single lines (|) are marked. A list of these technical differences and their causes is provided in Appendix B.
The following editorial changes have been made to this document.
---The name of the standard is modified to "Determination of Microbial Degradability of Leather and Fur";
---Change the unit of CO2 content from "ppm" to "mg/kg";
---Change "tannery wastewater" and "urban sewage" to "inoculant";
---Change the untitled bar in "6.2 Stock Solution" to column form;
---Deleted the second-level title of "6.3 Minimum Medium";
--- Clarified the meaning of y and x in the standard curve equation of the calibration system of Equipment B;
---Deleted the formula numbers for chemical reaction equations and the corresponding relationships between different quantities;
---Changed the letter codes in some formulas;
---Added informative appendix A and appendix B;
---Changed the upper temperature limit during the test process of Method B in Appendix B of ISO.20136.2020;
---Deleted "References".
Please note that some content in this document may be subject to patents. The publisher of this document assumes no responsibility for identifying patents.
This document is proposed by China Light Industry Federation.
This document is under the jurisdiction of the National Leather Industry Standardization Technical Committee (SAC/TC252).
This document was drafted by. Sichuan University, Shaanxi University of Science and Technology, National Textile and Clothing Product Quality Inspection and Testing Center (Tongxiang, Zhejiang), Guangzhou
Dongguan Product Quality Supervision and Inspection Research Institute, Shenzhen Beichai Testing Technology Co., Ltd., Longfeng Lemei Fashion Co., Ltd., Dongguan Zhongbiao Branch
Technology Co., Ltd., China Light Inspection and Certification (Jinjiang) Co., Ltd., China Light Inspection and Certification Co., Ltd., and China Leather and Shoemaking Research Institute Co., Ltd.
The main drafters of this document. Wang Yanan, Qiang Taotao, Shen Jinyu, Han Jianjian, Zhou Xinguang, Feng Xuewei, Zhang Wenhua, Zhou Yehua, Wan Yaozhu,
Ding Yang, Sang Jun, Bu Qiaoqiao, Zhang Yanan.
Determination of microbial degradability of leather and fur
1 Scope
This document describes a test method for the microbial degradability of leather and fur panels.
This document is applicable to the determination of microbial degradability of various types of leather and fur panels.
2 Normative reference documents
This document has no normative references.
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
inoculum
Tannery wastewater containing activated sludge.
Note. If there is no tannery nearby, use urban sewage containing activated sludge from the urban sewage treatment plant as the inoculant.
4 Symbols and abbreviations
The following symbols and abbreviations apply to this document.
atm. standard atmospheric pressure, 101325Pa.
Ba(OH)2.barium hydroxide.
C. Carbon.
CO2.carbon dioxide.
GL14.Thread used with H-SAV29/32 Erlenmeyer flask (2000mL).
GL18.Thread used with H-SAVH40/45 Erlenmeyer flask (5000mL).
H-SAV29/32.The inner and outer diameters of the conical flask orifice are 29mm and 32mm respectively.
H-SAVH40/45.The inner diameter and outer diameter of the Erlenmeyer flask orifice are 40mm and 45mm respectively.
IR. Infrared spectrum (Infrared).
Qnar. The molar flow rate of air passing through the system per hour, in moles per hour (mol/h).
QnCO2.The molar flow rate of CO2 passing through the system per hour, in moles per hour (mol/h).
5 principles
5.1 General
Under the action of microorganisms in the inoculant, the collagen in the leather or fur sample is degraded to produce CO2.Through elemental analysis and titration
The initial carbon content in the sample and the cumulative amount of CO2 produced during the degradation process are measured using the method (or IR method), and the biodegradation rate is calculated.
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