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Determination of geniposidic acid in extract of eucommia ulmoides oliver leaf - HPLC
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Basic data
| Standard ID | GB/T 40644-2021 (GB/T40644-2021) |
| Description (Translated English) | Determination of geniposidic acid in extract of eucommia ulmoides oliver leaf - HPLC |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B04 |
| Word Count Estimation | 6,691 |
| Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration |
GB/T 40644-2021: Determination of geniposidic acid in extract of eucommia ulmoides oliver leaf - HPLC
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of geniposidic acid in extract of eucommia ulmoides oliver leaf - HPLC
ICS 65.020.01
CCSB04
National Standards of People's Republic of China
Detection of geniposide acid in the extract of Eucommia ulmoides leaves
High performance liquid chromatography
HPLC
Released on 2021-10-11
2022-05-01 implementation
State Administration for Market Regulation
Issued by the National Standardization Management Committee
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents"
Drafting.
Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying patents.
This document was proposed by the State Forestry and Grassland Administration.
This document is under the jurisdiction of the National Forest Chemical Products Standardization Technical Committee (SAC/TC558).
Drafting organizations of this document. Beijing Forestry University, China National Institute of Standardization, Zhejiang Shengshi Biotechnology Co., Ltd., Hebei Guanzhuo Testing Division
Technology Co., Ltd., Shenzhen Brand Building Promotion Center, Zhongshan Hongli Health Food Industry Research Institute, Chinese Academy of Forestry
Institute of Chemical Industry, Nanjing Forestry University, Infinitus (China) Co., Ltd.
The main drafters of this document. Lei Jiandu, Xi Xingjun, Liu Jing, Wang Luying, Chen Yongjian, Zhang Jingxuan, Shen Xiaoyu, Yang Zhihua, Zheng Ping, Wang Chengzhang,
Huang Chaobo, Lan Tao, Li Wenjun, Yang Zhongzhi, Sun Hongmei.
Detection of geniposide acid in the extract of Eucommia ulmoides leaves
High performance liquid chromatography
1 Scope
This document describes the principle, reagents and materials, instruments and equipment for the determination of geniposide acid content in Eucommia ulmoides leaf extract by high performance liquid chromatography.
Preparation, determination steps, calculation and presentation of results, repeatability, precision, and recovery of standard addition.
This document is applicable to the determination of geniposide acid content in Eucommia ulmoides leaf extract.
2 Normative references
The content of the following documents constitutes an indispensable clause of this document through normative references in the text. Among them, dated quotations
Only the version corresponding to the date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to
This document.
GB/T 6682 Analytical laboratory water specifications and test methods
3 Terms and definitions
There are no terms and definitions that need to be defined in this document.
4 Principle
In the same analysis cycle, adjust the mobile phase ratio according to a certain time program to make the key components in the Eucommia leaf extract according to their respective appropriate
The capacity factor achieves a good separation purpose.
The external standard method corresponding to geniposide acid was used for direct quantification, and the chromatographic column used was a reversed-phase C18 column.
Note. Geniposide acid, also known as geniposide acid, geniposide acid or geniposide acid, is an iridoid glucoside.
The ultraviolet spectrometry of geniposide acid shows that its maximum ultraviolet absorption wavelength is 240nm, so 240nm is determined as geniposide acid
HPLC detection wavelength.
5 Reagents and materials
Unless otherwise stated, only use reagents confirmed to be analytically pure and water that meets GB/T 6682 level 1 in the analysis.
5.1 Reagents
5.1.1 Methanol (CH3OH), chromatographically pure.
5.1.2 Phosphoric acid (H3PO4), the content is 85%.
5.2 Standard products
Geniposide standard product. purity ≥98%.
5.3 Geniposide acid standard stock solution
Weigh.200mg (accurate to 0.001g) of geniposide acid standard, put it in a 100mL volumetric flask, dissolve it with methanol and make it to volume
...
