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Determination of melittin content in bee venom powder - High performance liquid chromatography
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Basic data
| Standard ID | GB/T 40486-2021 (GB/T40486-2021) |
| Description (Translated English) | Determination of melittin content in bee venom powder - High performance liquid chromatography |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B47 |
| Word Count Estimation | 10,197 |
| Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration |
GB/T 40486-2021: Determination of melittin content in bee venom powder - High performance liquid chromatography
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of melittin content in bee venom powder - High performance liquid chromatography
ICS 65.140
CCSB47
National Standards of People's Republic of China
Determination of bee venom hemolytic peptide content in bee venom dry powder
High performance liquid chromatography
Released on 2021-08-20
2022-03-01 implementation
State Administration of Market Supervision and Administration
Issued by the National Standardization Management Committee
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents"
Drafting.
Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying patents.
This document was proposed by the All-China Federation of Supply and Marketing Cooperatives.
This document is under the jurisdiction of the National Bee Product Standardization Working Group.
Drafting organizations of this document. Institute of Bee Research, Chinese Academy of Agricultural Sciences, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences.
The main drafters of this document. Li Yi, Zhou Jinhui, Huang Jingping, Wang Peng, Zhang Yongxin.
Determination of bee venom hemolytic peptide content in bee venom dry powder
High performance liquid chromatography
1 Scope
This document specifies the high performance liquid chromatography analysis method for the determination of bee venom hemolytic peptide content in bee venom dry powder.
This document is applicable to the determination of bee venom hemolytic peptide content in bee venom dry powder.
2 Normative references
The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations
Only the version corresponding to that date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to
This document.
GB/T 6682 Analytical laboratory water specifications and test methods
3 Terms and definitions
There are no terms and definitions that need to be defined in this document.
4 Principle
Bee venom hemolytic peptide is the main biologically active substance of bee venom. The bee venom hemolytic peptide in the sample is easily soluble in water, and the maximum ultraviolet absorption wavelength is
220nm. Therefore, the bee venom dry powder sample is dissolved in pure water, and the extract is filtered and measured by a high-performance liquid chromatograph equipped with an ultraviolet detector.
Quantification by external standard method.
5 Reagents and materials
The water is the first grade water that meets the requirements of GB/T 6682.
5.1 Reagents
5.1.1 Acetonitrile. chromatographically pure.
5.1.2 Trifluoroacetic acid. chromatographically pure.
5.2 Standard products
Bee venom hemolytic peptide (melittin, CAS. 20449-79-0) purity ≥ 97%.
5.3 Preparation of solution
5.3.1 Bee venom hemolytic peptide standard stock solution (10 mg/mL). accurately weigh 100 mg of the standard substance of bee venom hemolytic peptide into a 10 mL volumetric flask
In the medium, dissolve and dilute to the mark with water.
5.3.2 Bee Venom Hemolytic Peptide Standard Working Solution. Measure appropriate amount of standard stock solution and dilute with water to a mass concentration of 20 μg/mL,
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