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GB/T 40251-2021 PDF English

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GB/T 40251-2021: Code of diagnosis for MSX disease of oysters - In situ hybridization method
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GB/T 40251-2021English259 Add to Cart 3 days [Need to translate] Code of diagnosis for MSX disease of oysters - In situ hybridization method

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Basic data

Standard ID GB/T 40251-2021 (GB/T40251-2021)
Description (Translated English) Code of diagnosis for MSX disease of oysters - In situ hybridization method
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B41
Word Count Estimation 14,140
Issuing agency(ies) State Administration for Market Regulation, China National Standardization Administration

GB/T 40251-2021: Code of diagnosis for MSX disease of oysters - In situ hybridization method



---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Code of diagnosis for MSX disease of oysters-In situ hybridization method ICS 65.020.30 CCSB41 National Standards of People's Republic of China Diagnostic procedures for monosporidiosis of oysters In situ hybridization Released on 2021-05-21 2021-12-01 implementation State Administration of Market Supervision and Administration Issued by the National Standardization Management Committee

Foreword

This document is in accordance with GB/T 1.1-2020 "Guidelines for Standardization Part 1.Structure and Drafting Rules of Standardization Documents". To be drafted. Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying patents. This document was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China. This document is under the jurisdiction of the National Fisheries Standardization Technical Committee (SAC/TC156). Drafting organizations of this document. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, National Fisheries Technology Extension Station. The main drafters of this document. Bai Changming, Yang Bing, Wang Chongming, Huang Chi, Li Qing, Wan Xiaoyuan, Xin Lusheng, Li Chen, Yu Weizhong. Diagnostic procedures for monosporidiosis of oysters In situ hybridization

1 Scope

This document specifies the requirements for the location method of the pathogen in the diagnostic protocol for oyster monosporidiosis. (Haplosporidiumnelsoni), which gives the reagents or materials, equipment, samples, test procedures and results required for the in situ hybridization detection method determination. This document is applicable to the assessment and diagnosis of the degree of infection of Monospora niseri in oysters; other shellfish can also be implemented by reference.

2 Normative references

The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations Only the version corresponding to that date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to This document. SC/T 7205.1-2007 Diagnosis of Nanoworm Disease in Oyster Part 1.Cytological Diagnosis of Tissue Print

3 Terms and definitions

There are no terms and definitions that need to be defined in this document.

4 Abbreviations

The following abbreviations apply to this document. AP. alkaline phosphatase (alkalinephosphatase) BCIP. 5-bromo-4-chloro-3-indolylphosphatep-toluidinesalt DAFA. Davidson's alcohol-formalin-acetic acid fixative (davidson'salcohol-formalin-acetic acid fixative) DIG. Digoxin (digoxigenin) EDTA-Na2·2H2O. Ethylenediaminetetraacetic acid, disodiumdi- hydrate) MgCl2·6H2O. magnesium chloride hexahydrate NaCl. sodium chloride (sodiumchloride) Na2HPO4 12H2O. sodium phosphate dibasicdodecahydrate Na3C6H5O7 2H2O. Trisodium Citrate Dihydrate (sodium citratedihydrate) NBT. 4-Nitrobluetetrazoliumchloride KH2PO4.potassium dihydrogen phosphate (potassiumphosphatemonobasic) KCl. potassium chloride (potassiumchloride) PBS. phosphate buffer (phosphatebuffersalinebuffer) SSC. sodium citrate buffer (salinesodium citrate buffer) Tris. Tris[hydroxymethyl]aminomethane ...

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