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GB/T 36921-2018: Determination of vitamin B2, vitamin B3 and vitamin B6 in toothpaste -- High performance liquid chromatography
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GB/T 36921-2018: Determination of vitamin B2, vitamin B3 and vitamin B6 in toothpaste -- High performance liquid chromatography

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Determination of vitamin B2, vitamin B3 and vitamin B6 in toothpaste--High performance liquid chromatography ICS 71.100.40 Y43 National Standards of People's Republic of China Vitamin B2, vitamin B3 and toothpaste Determination of vitamin B6 by high performance liquid chromatography Published on.2018-12-28 2019-07-01 implementation State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard was proposed by the China Light Industry Association. This standard is under the jurisdiction of the National Dental Care Products Standardization Technical Committee Toothpaste Subcommittee (SAC/TC492/SC1). This standard was drafted. Suzhou Quality Inspection Science Research Institute, Haolai Chemical (Zhongshan) Co., Ltd., Perfect (China) Co., Ltd., Guangzhou Quality Supervision and Inspection Institute, Suzhou Shipu Detection Technology Co., Ltd., Guangdong Huaxin Testing Technology Co., Ltd., Jiangsu Food and Drug Administration Inspection Institute, Jiangsu Provincial Product Quality Supervision and Inspection Institute, Hebei Food Inspection Institute. The main drafters of this standard. Che Wenjun, Li Xiaomin, Huang Feng, Zhang Zheng, Huang Rongrong, Fang Ping, Yuan Liwen, Liu Yuan, Liao Huayong, Guo Xindong, Lu Jian, Wu Zhongping, Hou Xiangyu, Zhang Yan. Vitamin B2, vitamin B3 and toothpaste Determination of vitamin B6 by high performance liquid chromatography

1 Scope

This standard specifies the method for determination of vitamin B2, vitamin B3 and vitamin B6 in toothpaste. This standard applies to the determination of vitamin B2, vitamin B3 and vitamin B6 in toothpaste.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods

3 Principle

The sample was extracted with water, precipitated with acetone, determined by high performance liquid chromatography, and characterized by retention time and quantified by external standard method.

4 reagents or materials

4.1 Water. Primary water specified in GB/T 6682. 4.2 Concentrated hydrochloric acid. analytically pure. 4.3 Formic acid. liquid chromatography grade. 4.4 Methanol. Liquid chromatography grade. 4.5 Acetone. liquid chromatography grade. 4.6 Sodium heptane sulfonate. excellent grade. 4.7 Acetone solution. Accurately measure 10 mL of acetone (4.5) and 90 mL of water and mix. 4.8 Hydrochloric acid solution. Accurately measure 100 mL of concentrated hydrochloric acid (4.2) and slowly pour into 100 mL of water and mix. 4.9 Sodium heptane sulfonate solution. Accurately weigh 1.0 g of sodium heptane sulfonate (4.6), dissolve it in 950 mL of water, adjust to formic acid (4.3) to pH=2.5, make up to 1L with water. Filter through a 0.45 μm filter. 4.10 Reference substance. vitamin B2 (riboflavin), vitamin B3 (nicotinic acid, nicotinamide) and vitamin B6 (pyridoxal hydrochloride, pyridoxine, pyridinium hydrochloride Indoleamine, purity ≥99%, its Chinese name, English name, CAS registration number, molecular formula, see Table A.1 in Appendix A. 4.11 riboflavin stock solution (0.25mg/mL). weigh 25mg (accurate to 0.1mg) riboflavin reference substance (4.10) with analytical balance, add 2 mL of hydrochloric acid solution (4.8), after sonication, transfer to a 100 mL brown volumetric flask with water and bring to volume. Stock solution at 0 ° C ~ Store at 4 ° C in the dark and store for 3 months. 4.12 Nicotinic acid, nicotinamide, pyridoxal, pyridoxine, pyridoxamine stock solution (1mg/mL each). accurately weigh 0.1g (accurate to 0.1mg) phase The corresponding reference substance (4.10) should be placed in a 100 mL brown volumetric flask, dissolved in water, and dilute to the mark to prepare a concentration of 1 mg/mL. Stock liquid. The stock solution is stored at 0 ° C ~ 4 ° C in the dark, and can be stored for 3 months. Note. When pyridoxal hydrochloride or pyridoxamine hydrochloride is used as a reference, the concentration of the solution is converted to pyridoxal or pyridoxamine. ...