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Determination of barbaloin in oral care and cleansing products
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Basic data
| Standard ID | GB/T 35831-2018 (GB/T35831-2018) |
| Description (Translated English) | Determination of barbaloin in oral care and cleansing products |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | Y43 |
| Classification of International Standard | 71.100.40 |
| Word Count Estimation | 10,110 |
| Date of Issue | 2018-02-06 |
| Date of Implementation | 2018-09-01 |
| Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration |
GB/T 35831-2018: Determination of barbaloin in oral care and cleansing products
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Determination of barbaloin in oral care and cleansing products
ICS 71.100.40
Y43
National Standards of People's Republic of China
Determination of aloin in oral care products
Published on.2018-02-06
2018-09-01 Implementation
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
China National Standardization Administration released
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed by China Light Industry Federation.
This standard is under the jurisdiction of the National Oral Care Products Standardization Technical Committee (SAC/TC492).
This standard was drafted by. Chongqing Dengkang Oral Care Products Co., Ltd., National Light Industry Toothpaste Wax Product Quality Supervision and Testing
Xin, Guangzhou Quality Supervision and Inspection Institute.
The main drafters of this standard. Huang Yi, Dong Haide, Zheng Wei, Guo Changhong, and Liu Wenyu.
Determination of aloin in oral care products
1 Scope
This standard specifies the principles, reagents and materials, apparatus and equipment, analysis procedures, and procedures for the determination of aloin in oral care products.
Calculations, detection limits, recoveries, and precision.
This standard uses high performance liquid chromatography and is suitable for the determination of aloin in oral care products.
This standard has a detection limit of 26 ng/mL for aloin.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article
Pieces. For undated references, the latest version (including all amendments) applies to this document.
GB/T 6682-2008 Analytical laboratory water specifications and test methods
3 Methodology
Aloin, also known as aloin or aloe-emodin, English name Aloin or Barbaloin, molecular formula C21H22O9. Aloe products
Aloin is present as a mixture of optical isomers aloin A and B. This standard evaluates the content of aloin A and B as a mixture
The aloe glucoside index of the product.
In this method, the sample is dissolved in methanol, vortexed, ultrasonically extracted from the aloin, centrifuged and filtered to take the supernatant with UV
The detector or diode array detector is analyzed by high performance liquid chromatography and quantified by the peak area external standard method.
4 Reagents and materials
Unless otherwise indicated, only analytically pure reagents and primary water specified in GB/T 6682-2008 shall be used in the analysis.
4.1 Methanol. Chromatographically pure.
4.2 Acetonitrile. Chromatographic purity.
4.3 Glacial acetic acid. Chromatographically pure.
4.4 1% glacial acetic acid (volume ratio). 1 mL glacial acetic acid 99 mL water.
4.5 The purity of aloin A reference substance is ≥98%, CAS number. 1415-73-2; the purity of aloin B reference substance is ≥98%, CAS number. 28371-
16-6.
5 Instruments and Equipment
5.1 High performance liquid chromatograph. equipped with UV detector or diode array detector.
5.2 Electronic analytical balance. accuracy 0.0001g.
5.3 sonicator.
5.4 Multiple Swirl Oscillators.
5.5 High-speed centrifuge. speed ≥ 10000r/min.
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