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GB/T 35024-2018: Detection method of mammals and poultry ingredients -- Suspended bead array
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GB/T 35024-2018: Detection method of mammals and poultry ingredients -- Suspended bead array

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(Common livestock and poultry animal composition detection method) ICS 67.120.10 B40 National Standards of People's Republic of China Common livestock and poultry animal composition detection method Liquid phase chip method Published on.2018-05-14 2018-12-01 implementation State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Biochip Standardization Technical Committee (SAC/TC421). This standard was drafted. China Academy of Inspection and Quarantine, Liaoning Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, the Chinese people State Council Shanghai Entry-Exit Inspection and Quarantine Bureau, Shandong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Chen Ying, Wu Yajun, Yang Yange, Han Jianxun, Liu Mingchang, Wang Bin, Huang Wensheng, Cao Jijuan, Wang Wei, Zhang Shuya, Gao Hongwei. Common livestock and poultry animal composition detection method Liquid phase chip method

1 Scope

This standard specifies the liquid phase chip detection method for common animal species components in livestock and poultry products. This standard applies to mammals and ruminant components in meat and processed products, milk, skin, viscera, animal feed and other livestock and poultry products, and 13 Animal species composition of common meat or adulterants (camel, horse, donkey, yak, buffalo, dog, pig, red deer, sheep, chicken, duck, rat, mouse) Sex detection. The minimum detection limit of the method is (mass percentage). buffalo 5%, breastfeeding, ruminant, dog, sheep, chicken, rat, mouse, 1%, camel, The minimum detection limit for yak, red deer, pig, horse, donkey and duck is 0.1%.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods GB/T 14699.1 Feed sampling GB/T 27403-2008 Laboratory quality control regulations Food molecular biology testing SN/T 3561 Frontier Health Supervision Food Sampling and Sample Delivery Procedure 3 terms and definitions, abbreviations 3.1 Terms and definitions The following terms and definitions apply to this document. 3.1.1 Liquid phase chip technology suspendedbeadarray The probe-coupled microspheres are bound to the target gene sequence in the sample by liquid phase hybridization, and various types in the sample are processed by flow cytometry. Target components are tested simultaneously. 3.1.2 Liquid phase chip probe suspendedarrayprobe A nucleic acid or protein molecule immobilized on the surface of the microsphere and capable of affinity-detecting the target component with a target component. 3.1.3 Quality control probe qualitycontrolprobe A probe used to monitor whether the chip reaction is normal. Note. This standard uses a synthetic oligonucleotide probe (polyT20polyA20, 5' amino label). 3.1.4 Medianfluoresenceintensity The fluorescence signal value of the sample detected by the liquid crystal chip detector, that is, the software automatically reads the number of microspheres combined with the sample, and performs background values. The value obtained after calibration. ...