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Genetic diversity analysis on aquatic germplasm by genomic DNA microsatellite markers
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Basic data
| Standard ID | GB/T 34748-2017 (GB/T34748-2017) |
| Description (Translated English) | Genetic diversity analysis on aquatic germplasm by genomic DNA microsatellite markers |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B50 |
| Classification of International Standard | 65.150 |
| Word Count Estimation | 14,198 |
| Date of Issue | 2017-11-01 |
| Date of Implementation | 2018-05-01 |
| Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China |
GB/T 34748-2017: Genetic diversity analysis on aquatic germplasm by genomic DNA microsatellite markers
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Genetic diversity analysis on aquatic germplasm by genomic DNA microsatellite markers
ICS 65.150
B50
National Standards of People's Republic of China
Microsatellite Analysis of Genomic DNA from Aquatic Germplasm Resources
Genetic diversity of aneuploidy mmplasmbygenomicDNAmicrosatelite
markers
Posted.2017-11-01
2018-05-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
China National Standardization Administration released
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some of this document may be patentable. The issuing agencies of this document do not bear the responsibility of identifying these patents.
This standard proposed by the People's Republic of China Ministry of Agriculture.
This standard by the National Aquatic Standardization Technical Committee Freshwater Culture Sub-Technical Committee (SAC/TC156/SC1) centralized.
This standard drafting unit. Chinese Academy of Fishery Sciences Heilongjiang Institute of Fisheries.
The main drafters of this standard. Kuang Friendship, Sun Xiaowen, Zheng Xianhu, Liang Liqun.
Microsatellite Analysis of Genomic DNA from Aquatic Germplasm Resources
1 Scope
This standard specifies the reagents and materials, instruments and equipment, analysis steps and number of microsatellite analysis of aquatic germplasm resources genomic DNA
According to analysis.
This standard applies to the microsatellite analysis of genomic DNA of aquatic germplasm resources.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 18654.15 farmed fish germplasm testing Part 15. RAPD analysis
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Microsatellites microsatellites
Short tandem repeats consisting of two or more nucleotides in the genome of the eukaryotic genome.
3.2
Microsatellite analysis of microsatellites
The microsatellite markers were used to amplify the genomic DNA. After the products were detected by gel electrophoresis, the electrophoresis patterns were analyzed and their
In the implication of genetic polymorphism information.
4 Reagents and materials
Except for the reagents and materials specified in GB/T 18654.15, the following reagents and materials are required by this standard. In points unless otherwise stated
Only the reagents identified as analytically pure and double-distilled water or de-ionized water or rather pure water are used in the analysis.
4.1 Deionized ultrapure water. Autoclave.
4.2 TEMED. Store at 4 ° C in refrigerator.
4.3 Microsatellite-labeled primer. Preparation method in line with Appendix A.
4.4 Acrylamide. DNA sequencing grade.
4.5 methylene bisacrylamide DNA sequencing grade.
4.6 urea.
4.7 Deionized formamide.
4.8 polyacrylamide gel storage solution, preparation method in accordance with Appendix A.
4.9 10% (mass ratio) of ammonium persulfate (APS) solution. Preparation method in accordance with Appendix A.
4.10 polyacrylamide gel fixing solution, preparation method in accordance with Appendix A.
4.11 polyacrylamide gel staining solution, preparation method in accordance with Appendix A.
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