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GB/T 34656-2017: Specifications for marine sediment interstitial biota survey
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GB/T 34656-2017: Specifications for marine sediment interstitial biota survey

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Specifications for marine sediment interstitial biota survey ICS 07.060 A45 National Standards of People's Republic of China Marine sediment gap biological survey specification 2017-10-14 Published 2017-10-14 Implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released Directory Introduction Ⅶ Introduction Ⅷ 1 Scope 1 2 Normative references 1 3 Terms and definitions 1 4 General Provisions 2 4.1 Technical Design 2 4.2 The basic requirements of the investigation 2 4.3 Sampling 3 4.4 Sample Analysis 3 4.5 The basic requirements for data collation 4 4.6 Survey Results 4 4.7 data archiving 5 4.8 Quality Planning and Quality Control 5 5 sediment survey 5 5.1 Method Overview 5 5.2 Technical Requirements 5 5.3 Sample Collection and Preservation 6 5.4 Determination of physical and chemical factors 6 5.5 Determination of sediment age 6 5.6 Sediments Determination of heavy metals, organic pollutants and oils 6 5.7 Determination of sediment particle size 6 5.8 Analysis of sediment mineral components 6 5.9 Classification of sediment types in offshore sediments 6 5.10 Classification of deep-sea sediment types 7 5.11 Sediment biological composition survey 7 5.12 data arrangement 7 6 Suspension survey 7 6.1 Method Overview 7 6.2 Technical Requirements 7 6.3 Suspension mass concentration measurement 8 6.4 Suspension site particle size and volume concentration measurement 8 6.5 Particulate organic carbon and organic nitrogen determination 9 6.6 Data Management 10 Foraminifera survey 10 7.1 Method Overview 10 7.2 Technical Requirements 7.3 Foraminifera sample collection and storage 10 7.4 Tools and Reagents 11 7.5 Foraminifera sample handling and analysis 11 7.6 Data Management 16 8 Ogata survey 16 8.1 Method Overview 16 8.2 Technical Requirements 8.3 Ostracoda sample collection and preservation 17 8.4 Tools and Reagents 17 8.5 Processing and analysis of ostracods sample 17 8.6 Data Management 18 9 Radioactive insect survey 19 9.1 Method Overview 19 9.2 Technical Requirements 19 9.3 Collection and preservation of radiolarian samples 19 9.4 Tools and Reagents 19 9.5 Radiolarian sample handling and analysis 20 9.6 Data Management 20 Sedimentary diatoms survey 10.1 Method Overview 20 10.2 Technical Requirements 10.3 Collection and preservation of sediment diatoms samples 21 10.4 Tools and Reagents 21 10.5 Sediment diatom sample handling and analysis 21 10.6 Data Management 22 Survey of 11 coccoliths 11.1 Method Overview 22 11.2 Technical Requirements 11.3 collection and preservation of algae samples 23 11.4 Tools and reagents 23 11.5 Treatment and analysis of coccolithia samples 23 11.6 Data Management 24 12 sedimentary pollen survey 25 12.1 Method Overview 25 12.2 Technical Requirements 12.3 Collection and preservation of deposited pollen samples 25 Tools and Reagents 25 12.5 Deposition of Pollen Samples and Analysis 25 12.6 Data Arrangement 27 13 Benthic virus survey 27 13.1 Method Overview 27 13.2 Technical Requirements 13.3 Collection and preservation of benthic virus samples 28 13.4 Tools and Reagents 28 13.5 Benthic virus sample handling and analysis 29 13.6 Data Arrangement 32 Benthic microbiology survey 14.1 Method Overview 32 14.2 Technical Requirements 14.3 Collection and preservation of benthic microorganism samples 33 14.4 Tools and Reagents 34 14.5 Processing and analysis of benthic microorganism samples 14.6 Data Management 43 Benthic microalgae survey 15.1 Method Overview 43 15.2 Technical Requirements 15.3 Collection and preservation of benthic microalgae samples 43 15.4 Tools and Reagents 45 15.5 Processing and analysis of benthic microalgae samples 45 15.6 Data Arrangement 49 Benthic Protozoan Survey 16.1 Method Overview 49 16.2 Technical Requirements 16.3 Collection and preservation of benthic protozoan samples 50 16.4 Tools and Reagents 51 16.5 Processing and analysis of benthic protozoan samples 53 16.6 Data Management 61 Survey of small benthic animals 61 17.1 Method Overview 61 17.2 Technical Requirements 17.3 Intertidal and shallow sea sampling 61 17.4 Deep sea sampling 61 17.5 Processing and Analysis of Small Benthic Processes Samples 63 17.6 Data Management 65 Appendix A (Informative) Sediment biomes of several major groups Figure 68 Appendix B (informative) Sediment gap biological investigation of several stratified sampling equipment Figure 70 Appendix C (Informative) Sample Labels and Several Sample Records 71 Appendix D (informative) several microscopy records table 74 Appendix E (Normative) Calculation of common community parameters 79 Appendix F (Normative) Sediment Triangle Diagram Classification 83 Appendix G (Normative) Water measurement standards observation hierarchy table 86 Appendix H (informative) Rinse sample processing record of the deposition 87 Appendix I (normative) coccolithium survey of several characteristic grading 88 Appendix J (Normative) Sediment virus fluorescence microscopy flow chart 89 Appendix K (Normative) Microbiological survey of several sampling equipment Figure 90 Appendix L (Normative) Microbiological survey of filter devices and filter basket Figure 92 Appendix M (Normative) Microbenthos survey several technical flow chart 93 Appendix N (Normative) Formulation and formulation of various solutions 97 Reference 103 Figure A.1. Several major groups of microbenthos Figure A.2 Several major groups of small benthic metazoa Figure 69 Figure B.1 push quantitative layered sampler Figure 70 Figure B.2 rocker quantitative layered sampler Figure 70 Figure E.1 Biomass volume calculation for miniature benthic organisms Figure 81 Figure F.1 Triangle diagrammatic classification of Shepard clastic sediments 83 Figure F.2 Trigonometric classification of clastic sediments such as Folk 84 Figure F.3 Triangular graphic classification of deep-sea sediments 85 Figure J.1 Sediment virus count flow chart 89 Figure K.1 VanVeen Grab Dredger Figure 90 Figure K.2 TFO dredger Figure 90 Figure K.3 Sediment trap Figure 91 Figure L.1 Vacuum filtration system Figure 92 Figure L.2 Diaphragm basket Figure 92 Figure M.1 benthic micro-algae and bacterial sample interception and screening technology flow chart 93 Figure M.2 benthic micro-algae and bacteria fluorescence staining technology flow chart 94 Figure M.3 Ludox Centrifugal Separation Technology Flow Chart 94 Figure M.4 Quantitative protein silver staining (QPS) technology flow chart 95 Figure M.5 denaturing gradient gel electrophoresis (DGGE) technology flow chart 95 Figure M.6 Clone Library Technical Flowchart 96 Table 1 Foraminifera PCR amplification primers 14 Table 2 benthic microorganism PCR amplification primers 36 Table 3 benthic microorganisms high-throughput sequencing amplification primers 40 Table 4 Real-time fluorescence quantitative PCR amplification primers 41 Table C.1 Sample Labels 71 Table C.2 Sampling Records Table 72 Table C.3 Small Benthic Processes Sampling Records 73 Table D.1 benthic virus, bacterial fluorescence microscope count record table 75 Table D.2 benthic virus, bacterial flow cytometry record sheet 76 Table D.3 Microbenthos microscopic examination records 77 Table D.4 Small benthic metazoa counts Table 78 Table E.1 Biomass conversion factor table 80 Table G.1 Water body measurement standard observation hierarchy Table 86 Table H.1 Stamping record sheet 87 Table I.1 Crate Preservation Feature Hierarchy 88 Table I.2 Relative abundance characteristics of all coccoliths in sediments 88 Table N.1 Preparation of f/2 broth Table 101 Table N.2 Formulation of SWII Medium Table 101 Table N.3 Benthic Protozoa Investigation Table 102 of different concentrations of denaturing solutions in DGGE Table N.4 Lysis Buffer Formulation Table 102

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard proposed by the State Oceanic Administration. This standard by the National Marine Standardization Technical Committee (SAC/TC283) centralized. This standard drafting unit. Institute of Oceanology, Chinese Academy of Sciences, Tongji University, China Geological Survey Qingdao Institute of Marine Geology, the State Ocean First Bureau of Oceanography, State Oceanic Administration, Second Institute of Oceanography, State Oceanic Administration Third Institute of Oceanography, Chinese Academy of Sciences South China Sea Institute, China University of Geosciences (Beijing), Nanjing Institute of Geology and Palaeontology, Chinese Ocean University. The main drafters of this standard. Class Yan Li, Xu Kuidong, Sun Song, Li Tigang, 翦 know 湣, Liu Jian, Li Ruixiang, Wang Chunsheng, Luo Zhuhua, Chen Muhong, Su Xin, Li Baohua, Qiao Lulu.

Introduction

Marine sediment gap organisms cover six "circles" of life forms in the three-domain taxonomy of organisms. Archaea, Bacteriological, Protozoan Material, pigment, fungal and animal kingdoms, as well as benthic viruses. Sediment gap organisms are estuaries, intertidal zone, shelf shallow sea to The largest and most complex living groups in deep-sea benthic ecosystems are the material basis and energy flow for sustaining the operation of benthic life systems The essential. Marine sediment gap organisms through conventional means of ocean survey is not easy to obtain, the development of this standard is to fill the national standard GB/T 12763 "ocean survey norms" lack of investigation of sedimentary system requirements. In order to adapt to the development of modern marine science and technology and improve the contents of marine surveys in China, the State Oceanic Administration has organized the national standard "Marine Sedimentation Species Interval Biological Survey Norms "for the development of marine biodiversity, the exploration and utilization of marine strategic biological resources, integrated deep-sea resources and the environment Exploration, monitoring and evaluation of ecological environment, protection and management provide theoretical basis and technical support. Marine sediment gap biological survey specification

1 Scope

This standard determines the marine sediment gap biological investigation of the basic requirements established by the sediment, suspended solids, foraminifera, ostracods, put Bursts, sedimentary diatoms, coccolithiasis, sedimentary pollen, benthic viruses, benthic microorganisms (including bacteria, archaea and fungi), benthic microalgae, primitive benthic movements Objects, small benthic animals and other investigation of the general provisions and methods. This standard applies to coastal areas, shallow seas and deep sea offshore and distant seas of different marine benthos survey.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 12763.1 Marine survey code - Part 1. General principles GB/T 12763.4 Code of practice for marine survey - Part 4. Survey of chemical elements of seawater GB/T 12763.6 Marine survey code - Part 6. Marine life survey GB/T 12763.8 Marine survey code - Part 8. Marine geophysical survey GB 17378.4 Marine monitoring code Part 4. Seawater analysis

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Marine sediment marinesediment Weathering products of parent rocks (magmatic rocks, metamorphic rocks, pre-existing sedimentary rocks) under the influence of the surface crustal geology, biogenic materials, volcanoes The original materials of matter, cosmic matter and so on have been loose, unconsolidated on the coast or the seafloor after being transported, settled or precipitated Accumulation. 3.2 Sedimentary rock sedimentaryrock It is one of the three major types of rocks that make up the lithosphere (the other two are magmatic rocks and metamorphic rocks). It consists of the parent rock (Or any pre-rock) weathering products, biogenic materials, volcanic materials, cosmic materials and other raw materials after the removal of the role of sedimentation And sedimentary diagenesis and the formation of rocks. 3.3 Sediment interstitial biota interstitialbiota All benthic life forms that inhabit or deposit in the interstices between sediment particles. Notes include marine microorganism, benthicvirus, microbenthos, miniature and small Type benthic metazoan (micro-and meiobenthicmetazoa) and so on. From the individual size, marine sediment gap bio-covers a particle size of less than 0.2 μm (Femto-grade) of 0.2 μm to 2 μm, pico-order of 0.2 μm to 2 μm, nano-order of 2 μm to 20 μm and micro Micro-level single-cell benthos and meio-level benthic metazoans. ...