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Detection of genetically modified components in maize -- Gene chip method
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Basic data
| Standard ID | GB/T 33807-2017 (GB/T33807-2017) |
| Description (Translated English) | Detection of genetically modified components in maize -- Gene chip method |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B04 |
| Classification of International Standard | 67.050 |
| Word Count Estimation | 14,128 |
| Date of Issue | 2017-05-31 |
| Date of Implementation | 2017-12-01 |
| Quoted Standard | GB/T 6682; GB/T 19495.1; GB/T 19495.2; GB/T 19495.3; GB/T 19495.6; GB/T 19495.7 |
| Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China |
| Summary | This standard specifies the detection methods for transgenic components in maize and maize processed products. This standard is suitable for qualitative detection of transgenic elements of transgenic EPSPS gene, PAT gene, BAR gene herbicide tolerant maize and transgenic Bt gene (Cry1A105, Cry1Ab), and also for the qualitative detection of transgenic components in maize processed products. The minimum limit for detection of transgenic components is 0.1%. |
GB/T 33807-2017: Detection of genetically modified components in maize -- Gene chip method
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Detection of genetically modified components in maize - Gene chip method
ICS 67.050
B04
National Standards of People's Republic of China
Determination of Transgenic Genes in Maize
Gene chip method
Detection ofgeneticalymodifiedcomponentsinmaize-
Genechipmethod
2017-05-31 released
2017-12-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
China National Standardization Management Committee released
Foreword
This standard is drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and centralized by the National Standardization Technical Committee for Biochip (SAC/TC421).
The main drafting unit of this standard. Sichuan Huahan three biological technology Co., Ltd., the National Agricultural Standardization Monitoring and Research Center (Black Dragon
Jiang), Guangdong Product Quality Supervision and Inspection Research Institute, Heilongjiang Province, grain and oil health inspection and monitoring station.
The main drafters of this standard. Huang Guangping, Peng Liping, She Shenyun, Zhang Jinlong, Wang Zhiqiang, Wang Yongqiang, Kong Luyi, Wei Minwei, Zhang Chunhong,
Yin Zhijian, Wang Haikuan.
Determination of Transgenic Genes in Maize
Gene chip method
1 Scope
This standard specifies the detection methods for transgenic components in maize and maize processed products.
This standard is applicable to transgenic EPSPS gene, PAT gene, BAR gene herbicide resistant maize and transgenic Bt gene (Cry1A105,
Cry1Ab) insect-resistant maize transgenic components of qualitative testing, but also for corn processing products in the qualitative detection of genetically modified ingredients, the standard
The minimum detection limit for the defined transgenic component is 0.1%.
2 normative reference documents
The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article
Pieces. For undated references, the latest edition (including all modifications) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
GB/T 19495.1 General requirements and definitions for the detection of genetically modified products
GB/T 19495.2 Technical requirements for laboratory testing of transgenic products
Method for extraction and purification of nucleic acid for detection of transgenic products GB/T 19495
GB/T 19495.6 Detection of genetically modified products by gene chip detection
Sampling and sample preparation methods for transgenic product testing GB/T
3 terms, definitions and abbreviations
3.1 Terms and definitions
GB/T 19495.1, GB/T 19495.6 Definitions of terms and definitions apply to this document.
3.2 abbreviations
The following abbreviations apply to this document.
BAR. derived from Bacillus Bacillusamyloliquefaciens glufosinate acetyltransferase gene (phosphinothricinacetyl-
transferasegene
bp. base pair (basepair)
CaMV35S-P. 35S promoter from the cauliflower mosaic virus (Promoterfromcauliflowermosaicvirus)
CaMV35S-T. 35S terminator from cauliflower mosaic virus (Terminationsequencefromcauliflowermo-
saicvirus
Cry1Ab. Bacillus thuringiensis Insecticidal protein Cry1Ab gene (BacillusthuringiensisInsecticidaltoxin
Cry1Abgene)
Cry1A105. Bacillus thuringiensis Insecticidal Protein Cry1A105 Gene (BacillusthuringiensisInsecticidaltoxin
Cry1A105gene)
...