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GB/T 32723-2016: Determination of soil microbial biomass -- Substrate-induced respiration method
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GB/T 32723-2016: Determination of soil microbial biomass -- Substrate-induced respiration method

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Determination of soil microbial biomass - Substrate-induced respiration method ICS 13.080.30 B10 National Standards of People's Republic of China Determination of Soil Microbial Biomass Substrate - induced respiration (ISO 14240-1..1997, Soilquality-Determination ofsoilmicrobialbiomass- Part 1. Substrate-inducedrespirationmethod, IDT) 2016-08-29 released 2017-03-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China China National Standardization Management Committee released

Foreword

This standard is drafted in accordance with the rules given in GB/T 1.1-2009. This standard uses the translation method equivalent to ISO 14240-1..1997 "Soil quality Soil microbial biomass determination Part 1. Substrate-induced respiration ". For ease of use, this standard has been modified as follows. --- Change the standard name to "Soil microbial biomass determination of substrate-induced respiration". This standard is proposed by the Ministry of Agriculture of the People's Republic of China. This standard is under the responsibility of the National Soil Quality Standardization Technical Committee (SAC/TC404). The drafting of this standard. Institute of Subtropical Agriculture, Chinese Academy of Sciences, Nanjing Institute of Soil Science, Chinese Academy of Sciences, China Agricultural University, Jiangsu Provincial Institute of Standardization. The main drafters of this standard. Wu Jinshui, Lin Qimei, Lin Xiangui, Wei Wenxue, Chu Haiyan, Li Baochen, Chen Meijun, Luo Kun Determination of Soil Microbial Biomass Substrate - induced respiration

1 Scope

This standard specifies the principle, conditions, reagents and materials, instruments, steps and results of soil microbial biomass by substrate-induced respiration method. Calculation and reporting requirements. This standard applies to the determination of the activity of aerobic heterotrophic microbial biomass in well-ventilated agricultural soils and mineral soils. This standard does not apply to the determination of the effect of chemicals on soil microbial biomass.

2 normative reference documents

The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article Pieces. For undated references, the latest edition (including all modifications) applies to this document. ISO 10381-6..1993 Soil quality sampling - Part 6. Laboratory determination - Soil collection and treatment for aerobic microbial processes And storage guidelines (Soilquality-Sampling-Part 6. Guidanceonthecolection, handlingandstorageofsoil fortheassessmentofaerobicromicrobialprocessesinthelaboratory ISO 10390..1994 Determination of soil quality pH (Soilquality-Determination ofpH) ISO 11277..2002 Soil quality - Determination of particle size distribution in mineral soil Sieve and sedimentation method (Soilquality-Determi- nationofparticlesizedistributioninmineralsoilmaterial-methodbysievingandsedimentation ISO 11465..1993 Soil quality Quality determination of soil dry weight and water content Gravimetric method (Soilquality-De- terminationofdrymatterandwatercontentonamassbasis-Gravimetricmethod)

3 terms and definitions

The following terms and definitions apply to this document. 3.1 Soil microbial biomass Quality of Living Microbial Cells in Soil. Note. This indicator can be estimated by measuring the amount of carbon or nitrogen in these cells, or by measuring the mineralization capacity of the added carbon source. Such as the use of carbon or nitrogen content Analysis, it may include dead cells or cell debris, such as the determination of soil respiration, can only be measured to live cells. 3.2 Soil respiration rate soilrespirationrate The unit mass of soil releases CO2 volume per unit time.

4 principle

Add glucose to form a series of soil glucose concentration gradients until the highest respiration rate (typically no more than 1 h) is reached as the most Large initial respiration rate used to estimate soil active microbial biomass. ...