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Basic data
| Standard ID | GB/T 31270.7-2025 (GB/T31270.7-2025) |
| Description (Translated English) | Test guidelines on environmental safety assessment for chemical pesticides - Part 7: Bioconcentration test |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B15 |
| Classification of International Standard | 65.100 |
| Word Count Estimation | 30,385 |
| Date of Issue | 2025-10-31 |
| Date of Implementation | 2026-05-01 |
| Older Standard (superseded by this standard) | GB/T 31270.7-2014 |
| Issuing agency(ies) | State Administration for Market Regulation and Standardization Administration of China |
GB/T 31270.7-2025: Test guidelines on environmental safety assessment for chemical pesticides - Part 7: Bioconcentration test
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 65.100
CCSB15
National Standards of the People's Republic of China
Replaces GB/T 31270.7-2014
Environmental Safety Evaluation Test Guidelines for Chemical Pesticides
Part 7.Bioaccumulation Tests
pesticides-Part 7.Bioconcentrationtest
Implemented on May 1, 2026
State Administration for Market Regulation
The State Administration for Standardization issued a statement.
Table of contents
Preface III
Introduction V
1.Scope 1
2 Normative References 1
3.Terms and Definitions 1
4.Principle 2
5.Test conditions 3
6.Reagents and Materials 3
7.Instruments and Equipment 4
8 Test Subjects 4
9.Experimental Steps 4
10 Data Processing 9
11 Quality Control
12 Test Report 11
Appendix A (Normative) Test Fish Species and Their Body Length and Water Temperature Conditions 14
Appendix B (Normative) Physicochemical Parameters of Test Water 15
Appendix C (Informative) Selection of Fish Feed (Feeding Exposure Method) 16
Appendix D (Informative) Example 17 of Sampling Schedule
Appendix E (Normative) Water Quality Measurement and Sample Collection During the Test 19
References 21
Foreword
This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1.Structure and Drafting Rules of Standardization Documents".
Drafting.
This document is Part 7 of GB/T 31270, "Test Guidelines for Environmental Safety Evaluation of Chemical Pesticides". GB/T 31270 has been published.
The following section.
---Part 1.Soil Metabolism Test;
---Part 2.Hydrolysis Test;
---Part 3.Photolysis Test;
---Part 4.Soil Adsorption/Desorption Tests;
---Part 5.Soil Leaching Test;
---Part 6.Volatility Testing;
---Part 7.Bioaccumulation Assays;
---Part 8.Metabolic Experiments of Water-Sediment Systems;
---Part 9.Short-term feeding toxicity tests in birds;
---Part 10.Acute toxicity tests on bees;
---Part 11.Acute Toxicity Tests on Silkworms;
---Part 12.Acute Toxicity Testing in Fish;
---Part 13.Acute Activity Inhibition Test in Daphnia;
---Part 14.Algal Growth Inhibition Test;
---Part 15.Acute toxicity test of earthworms;
---Part 16.Soil Microbial Toxicity Testing;
---Part 17.Acute toxicity test of the natural enemy Trichogramma wasp;
---Part 18.Acute toxicity testing in natural enemy amphibians;
---Part 19.Experiments on the Effects of Non-Target Plants;
---Part 20.Short-term feeding toxicity studies in livestock;
---Part 21.Toxicity Testing of Large Crustaceans;
---Part 22.Photolysis Test of Soil Surface;
---Part 23.Acute oral toxicity test in birds.
This document replaces GB/T 31270.7-2014 "Test Guidelines for Environmental Safety Evaluation of Chemical Pesticides Part 7.Bioaccumulation Tests".
Compared with GB/T 31270.7-2014, the main technical changes in the "Experimental Standard" are as follows, except for structural adjustments and editorial modifications.
a) The dietary exposure method has been added (see Chapter 1, 4.2, 6.3, 9.2, Chapter 10, Chapter 11, and Appendix E);
b) The experimental principles were changed (see Chapter 4, Chapter 3 of the.2014 edition);
c) The test conditions were changed (see Chapter 5, 4.1.4 of the.2014 edition);
d) Requirements for reagents and materials have been added (see Chapter 6);
e) The requirements for test organisms have been changed (see 6.1 and Appendix A, 4.1.2 and Appendix A of the.2014 edition);
f) The requirements for test water have been changed (see 6.2 and Appendix B, 4.1.4 of the.2014 edition);
g) Major instruments and equipment have been modified (see Chapter 7, 4.1.3 of the.2014 edition);
h) The requirements for the test substance have been changed (see Chapter 8, 4.1.1 of the.2014 edition);
i) A pre-test method has been added (see 9.1.2);
j) The test procedures related to the water exposure method have been modified (see 9.1.3, 4.2.3 in the.2014 version);
k) The data statistics methodology has been changed (see Chapter 10, 4.3 of the.2014 edition);
l) The quality control conditions have been changed (see Chapter 11, 4.4 of the.2014 edition);
m) The test report requirements have been changed (see Chapter 12, Chapter 5 of the.2014 edition);
n) The body length and water temperature requirements for the tested organisms were changed (see Table A.1,.2014 edition of Table A.1);
o) The section on "Selection of Test Methods" in Appendix B has been deleted, and "Water Quality Indicators for Test Water" has been added (see Appendix B,.2014).
Appendix B of the.2017 edition);
p) Added requirements related to water quality testing and sample collection during the experiment (see Appendix E).
Please note that some content in this document may involve patents. The issuing organization of this document assumes no responsibility for identifying patents.
This document was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China.
This document is under the jurisdiction of the National Technical Committee on Pesticide Standardization (SAC/TC133).
This document was drafted by the Institute for the Control of Agrochemicals, Ministry of Agriculture and Rural Affairs.
The main drafters of this document are. Chen Lang, Yuan Ye, Song Wencheng, Zhang Zhang, Zhao Yuanji, Liu Xingang, Shu Linjun, Yu Jia, Zhu Lizhen, Mao Liangang, and Li Min.
The release history of this document and the document it replaces is as follows.
---First published in.2014 as GB/T 31270.7-2014;
---This is the first revision.
Introduction
To reduce the impact of pesticide use on the ecological environment and to provide data that meets the requirements of pesticide environmental risk assessment, an environmental safety assessment is necessary.
The experiment clarified the characteristics of pesticides in the environment, including metabolism, degradation, adsorption, leaching, and bioaccumulation, as well as their toxicity to non-target organisms. GB/T
31270, "Test Guidelines for Environmental Safety Evaluation of Chemical Pesticides," is a standard for test methods guiding environmental safety evaluation of pesticides in my country, aiming to standardize...
The pesticide environmental safety evaluation test, aimed at improving the scientific rigor and comparability of pesticide environmental safety evaluation tests, is planned to consist of 23 parts.
---Part 1.Soil Metabolism Experiment. The aim is to clarify the metabolic pathways and rates of pesticides in soil.
---Part 2.Hydrolysis Tests. The purpose is to clarify the hydrolysis pathways and rates of pesticides.
---Part 3.Photolysis Experiment. The purpose is to clarify the photolysis pathway and rate of pesticides.
---Part 4.Soil Adsorption/Desorption Tests. The purpose is to clarify the soil adsorption characteristics of pesticides.
---Part 5.Soil Leaching Test. The purpose is to determine the leaching properties of pesticides.
---Part 6.Volatility Test. The purpose is to determine the volatility of pesticides.
---Part 7.Bioaccumulation Test. The purpose is to determine the bioaccumulation of pesticides in fish.
---Part 8.Metabolic Experiments in Aquatic-Sediment Systems. The aim is to clarify the metabolic pathways and metabolism of pesticides in aquatic-sediment systems.
Thank rate.
---Part 9.Short-term feeding toxicity studies in birds. The aim was to determine the short-term feeding toxicity of pesticides to birds.
---Part 10.Acute toxicity tests on bees. The purpose is to determine the acute toxicity of pesticides to bees.
---Part 11.Acute toxicity test on silkworms. The purpose is to determine the acute toxicity of pesticides to silkworms.
---Part 12.Acute Toxicity Tests in Fish. The purpose is to determine the acute toxicity of pesticides to fish.
---Part 13.Acute activity inhibition test of Daphnia. The purpose is to determine the acute toxicity of pesticides to Daphnia.
---Part 14.Algal Growth Inhibition Tests. The purpose was to determine the toxicity of pesticides to algae.
---Part 15.Acute toxicity test on earthworms. The purpose was to determine the acute toxicity of pesticides to earthworms.
---Part 16.Soil Microbial Toxicity Testing. The purpose is to determine the toxicity of pesticides to soil microorganisms.
---Part 17.Acute toxicity test of the natural enemy Trichogramma wasp. The purpose was to determine the toxicity of pesticides to Trichogramma wasps.
---Part 18.Acute toxicity tests on natural enemies (amphibians). The purpose is to determine the acute toxicity of pesticides to amphibians.
---Part 19.Effects on Non-Target Plants. The aim is to determine the toxicity of pesticides to non-target plants.
---Part 20.Short-term feeding toxicity studies in livestock. The aim is to determine the feeding toxicity of pesticides to livestock.
---Part 21.Toxicity Testing on Large Crustaceans. The purpose is to determine the toxicity of pesticides to large crustaceans.
---Part 22.Photodegradation Experiments on Soil Surfaces. The purpose is to clarify the photodegradation pathways and rates of pesticides on soil surfaces.
---Part 23.Acute oral toxicity tests in birds. The purpose is to determine the acute oral toxicity of pesticides to birds.
Environmental Safety Evaluation Test Guidelines for Chemical Pesticides
Part 7.Bioaccumulation Tests
Warning---Personnel using this document should have practical experience working in a formal laboratory. This document does not address all possible safety issues.
Users are responsible for taking appropriate safety and health measures and ensuring compliance with relevant national regulations.
1 Scope
This document describes bioaccumulation test methods for environmental safety assessment of chemical pesticides, including water exposure and feeding exposure methods.
This document applies to bioaccumulation tests conducted for the registration of chemical pesticides, including bioaccumulation tests and bioscale-up tests.
2 Normative references
The contents of the following documents, through normative references within the text, constitute essential provisions of this document. Dated citations are not included.
For references to documents, only the version corresponding to that date applies to this document; for undated references, the latest version (including all amendments) applies.
This document.
GB/T 31270.12 Environmental Safety Evaluation Test Guidelines for Chemical Pesticides - Part 12.Acute Toxicity Tests in Fish
NY/T 4195.3 Environmental Impact Testing for Pesticide Registration - Biological Material Cultivation - Part 3.Zebrafish
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
bioconcentration
The process by which organisms continuously absorb test substances from water, leading to their gradual accumulation and concentration within their bodies.
Note. Also known as biological concentration.
3.2
biomagnification
When organisms ingest food containing the test substance, their concentration in the body becomes higher than that in the food, leading to the transfer and accumulation of the test substance through the food chain.
process.
3.3
bioaccumulation
The process by which organisms continuously absorb low doses of a test substance from the environment and/or food, leading to its gradual accumulation and concentration in the body.
3.4
semi-static test
The method of disinfection involves changing the disinfectant solution at regular intervals (e.g., 24 hours) during the experiment.
3.5
Flow-through test
The method of administering the drug solution was continuously updated during the experiment.
3.6
Spiked diet
The bait after adding the test substance in an appropriate manner and mixing it evenly.
...
